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A strain of Lactobacillus plantarum and its screening method for lowering cholesterol and nitrite

A kind of Lactobacillus plantarum, cholesterol-lowering technology, applied in the field of microorganisms, can solve the problem of no Lactobacillus plantarum, etc., and achieve the effects of improving the body's immunity, reducing the residual amount, and shortening the fermentation cycle.

Active Publication Date: 2018-09-21
北京中京丰创科技有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

These Lactobacillus plantarum have a certain cholesterol-lowering function, but so far, there is no application for Lactobacillus plantarum that has both cholesterol-lowering ability and nitrite-degrading ability

Method used

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  • A strain of Lactobacillus plantarum and its screening method for lowering cholesterol and nitrite
  • A strain of Lactobacillus plantarum and its screening method for lowering cholesterol and nitrite
  • A strain of Lactobacillus plantarum and its screening method for lowering cholesterol and nitrite

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Experimental program
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Embodiment

[0025] Example Screening of Lactobacillus plantarum GUFHSL 70 strains:

[0026] 1. Sample collection and strain isolation and purification

[0027] Samples were collected from Maocai and Maotang in Qiannan area, Guizhou Province. Take 10g or 10mL of the sample and put it into 90mL of sterile peptone water, shake it on the shaker for 30min, and take 200μL of the supernatant in the ultra-clean workbench and spread it on CaCO3- On the MRS agar plate, culture in a carbon dioxide incubator at 37°C for 48 hours, select a single colony that produces an obvious calcium dissolution circle for further purification, and then store it on the slant for future use.

[0028] 2. Colony morphology identification, physiological and biochemical identification and molecular biological identification

[0029] 2.1 The strain was cultured on the MRS plate for 24 hours, and the colony shape and color were observed and recorded.

[0030] 2.2 Gram staining: pick the colonies on the plate for smear, f...

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Abstract

A strain of Lactobacillus plantarum that lowers cholesterol and nitrite. This Lactobacillus plantarum is isolated from the traditional fermented pickled vegetables of ethnic minorities in Guizhou. The strain is named Lactobacillus paraplantarum GUFHSL‑70. The strain is pure The culture has been deposited in the China Center for Type Culture Collection on January 4, 2016. The address of the depository unit is: China, Wuhan, Wuhan University, and the deposit number is CCTCC M 2016002. Lactobacillus plantarum GUFHSL‑70 is a functional lactic acid bacterium with favorable properties in lowering cholesterol and nitrite. Develop it into health food or medicine, take it for a long time to improve the intestinal flora, lower serum cholesterol, and improve the body's immunity; or put it into fermented kimchi products, reduce the residual nitrite in kimchi, shorten the fermentation cycle, improve Product flavor.

Description

technical field [0001] The present invention relates to microorganisms, more specifically to lactic acid bacteria, in particular to lactic acid bacteria with both cholesterol-lowering ability and nitrite-degrading ability. Background technique [0002] According to medical research, elevated cholesterol levels in serum are considered to be an important risk factor for cardiovascular diseases such as coronary heart disease and atherosclerosis. The World Health Organization (WHO) predicts that cardiovascular disease will remain the leading cause of death until 2030, affecting 23 million people. Clinical studies have shown that exceeding the normal serum cholesterol level (>5.2mmol) by 1mmol increases the risk of coronary heart disease by 35%. At the same time, reducing the serum cholesterol level by 1% will reduce the risk of disease by 2% to 3%. [0003] Studies have found that acute nitrite poisoning leads to methemoglobinemia, and chronic poisoning has the risk of carci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/225
CPCC12N1/20C12N1/205C12R2001/225
Inventor 何腊平宋小娟李翠芹
Owner 北京中京丰创科技有限公司
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