Efficient microbial soil remediation agent

A technology of soil remediation agent and high-efficiency microorganisms, which is applied in the field of high-efficiency microbial soil remediation agents, can solve problems such as secondary pollution, unstable components, and poor effects, and achieve high concentration and stable quality

Inactive Publication Date: 2012-10-31
XUZHOU QIYUAN ECOLOGY AGRI DEV
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The products used to repair soil on the market are mainly chemical ingredients, which are likely to cause secondary pollution after use
Japan's EM bacteria are popularized in China, and the effect is not ideal, especially in the anti-stubble aspect. The main disadvantage of EM bacteria is that the composition is unstable. Antagonism is formed between them, only one strain of dominant bacteria grows up, and the effect is not good

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] 4 strains: Rhodopseudomonas palustris, Bacillus subtilis, Saccharomyces cerevisiae, Streptomyces jingyang.

[0022] The following are the secondary culture processes of the four strains:

[0023] Rhodopseudomonas palustris : The culture medium for primary culture is 1% yeast extract, 2% peptone, 2% glucose, the inoculum size is 2%, the culture pH is 6~10, the culture temperature is 36°C, the light intensity is 3500 lux, and the culture time is 5-8 days; the medium for the secondary culture is the same as that of the first-level culture, the inoculation amount is 15%, the culture pH is 6-10, the culture temperature is 36°C, the light intensity is 3000 lux, and the culture time is 6-8 days.

[0024] Bacillus subtilis : The medium for primary culture is 0.3% starch, 0.5% sucrose, 1.5% soybean powder, 0.25% dipotassium hydrogen phosphate, 0.2% manganese sulfate, 0.1% yeast extract, the inoculum size is 2%, and the culture pH is 6~8 , the culture temperature was 35°C, an...

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Abstract

The invention discloses an efficient microbial soil remediation agent, belonging to a propagation culture technique of microbes. The efficient microbial soil remediation agent is prepared by mixing the following components in percentage by volume: 10-25% of photosynthetic bacteria zymogen solution, 25-35% of Bacillus subtilis zymogen solution, 20-35% of microzyme zymogen solution and 20-40% of actinomycetes zymogen solution. The four strains are respectively subjected two secondary amplification culture under the conditions of proper temperature, ventilation, culture medium, pH value and culture time; and the culture process is carried out in a fermentation tank without foreign microbes. By using the modern biotechnology, single microbe strains are subjected to high-concentration pure culture and then compounded to produce the efficient microbial soil remediation agent.

Description

technical field [0001] The invention relates to a high-efficiency microbial soil restoration agent, which belongs to the technical field of microbial propagation and cultivation. technical background [0002] With the development of agricultural planting, more and more chemical fertilizers, pesticides and other chemical substances are accumulated in the farmland, resulting in soil compaction and many diseases that directly affect the yield and quality of agricultural products. This problem has become an urgent problem for the planting industry to overcome. . The products used to restore soil on the market are mainly chemical ingredients, which are likely to cause secondary pollution after use. Japan's EM bacteria are popularized in China, and the effect is not ideal, especially in the anti-stubble aspect. The main disadvantage of EM bacteria is that the composition is unstable. Antagonism is formed between them, and only one dominant bacterium grows up, and the effect is n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12N1/16B09C1/10C12R1/38C12R1/125C12R1/465C12R1/865
Inventor 李峰王延红
Owner XUZHOU QIYUAN ECOLOGY AGRI DEV
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