Large scale zhangzhi fungus deep fermenting process
A technology of liquid submerged fermentation and production process, applied in fungi and other directions, can solve the problem of no Antrodia camphorata and other problems, and achieve the effect of strong antioxidant capacity
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[0024] The mycelia of Antrodia camphorata were inoculated in the slant medium, the culture temperature was 28° C., and the culture time was 264 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaker at 28°C for 40 hours, and the shaker rotated at 150rpm / min. Then the obtained shake flask bacterial strain was transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, and cultured on a shaker at 28° C. for 22 hours at a shaker speed of 150 rpm / min. Then transfer 2200ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 50L seed culture medium, keep the tank temperature at 28°C, tank pressure at 0.7kPa, stirring rate at 100 rpm / min, ventilation rate at 1:0.5, The fermentation time is 45 hours. Transfer the primary seed tank bacteria into a 1500L fermenter equipped with 800L fermentation medium, keep the tank temperature at 28°C, tank...
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