Large scale zhangzhi fungus deep fermenting process

A technology of liquid submerged fermentation and production process, applied in fungi and other directions, can solve the problem of no Antrodia camphorata and other problems, and achieve the effect of strong antioxidant capacity

Inactive Publication Date: 2003-11-19
敖宗华 +2
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  • Application Information

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Embodiment 1

[0024] The mycelia of Antrodia camphorata were inoculated in the slant medium, the culture temperature was 28° C., and the culture time was 264 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaker at 28°C for 40 hours, and the shaker rotated at 150rpm / min. Then the obtained shake flask bacterial strain was transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, and cultured on a shaker at 28° C. for 22 hours at a shaker speed of 150 rpm / min. Then transfer 2200ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 50L seed culture medium, keep the tank temperature at 28°C, tank pressure at 0.7kPa, stirring rate at 100 rpm / min, ventilation rate at 1:0.5, The fermentation time is 45 hours. Transfer the primary seed tank bacteria into a 1500L fermenter equipped with 800L fermentation medium, keep the tank temperature at 28°C, tank...

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Abstract

A large-scale deep liquid fermenting process for preparing the mycelia of antrodia cinnamonea includes such steps as collecting the fungus of antrodia cinnamonea, liquid slant culture in shake flask, enlarging the culture, class-one seed culture, fermenting culture, and fermenting in (1000-5000)-L fermentor for 42 hr.

Description

technical field [0001] The invention relates to a large-scale liquid submerged fermentation production process of Antrodia camphorata. technical background [0002] Antrodia camphorata is a well-known traditional Chinese medicine in Taiwan. Wild fruiting bodies grow on the inner wall of the rotten heartwood of the trunk of the protected tree species of camphor tree in Taiwan, or on the dark and damp surface of the dead and fallen camphor wood, and are not easy to be found. Antrodia has a long history of use in Taiwan, and the aborigines regard Antrodia as the most precious medicinal material. The price of natural Antrodia camphorata is also rising day by day under the expectation that rare things are more expensive. It is currently known as the most expensive wild fungus in the world market, and the market price is often as high as NT$10 per catty. The price ranges from 10,000 yuan to hundreds of thousands of yuan, and the wild top grade can reach 15,000 US dollars per kil...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 敖宗华符慧子邹锡良
Owner 敖宗华
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