Large scale liquid deep fermentation for producing brilliant bacterial mycellium powder and its polysaccbaride technology

A technology of liquid submerged fermentation and production process, applied in horticulture, application, and botanical equipment and methods, etc., can solve the problems of easy contamination, low production efficiency, long production cycle, etc.

Inactive Publication Date: 2005-03-23
敖宗华 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the bright bacteria preparations on the market are produced by the solid culture method of bright bacteria, which has the disadvantages of long production cycle, low production efficiency and easy contamination.
At present, there is no report on the large-scale production of Leucobacterium mycelium powder and its polysaccharides by using a three-stage liquid fermentation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Inoculate the mycelium of Leucobacterium in the slant culture medium, the culture temperature is 28°C, and the culture time is 180 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaker at 28°C for 100 hours, and the shaker speed was 150rpm / min. Then the obtained shake flask bacterial strain was transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, and cultured on a shaker at 28° C. for 48 hours at a shaker speed of 150 rpm / min. Then transfer 2200ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 50L seed medium, keep the tank temperature at 28°C, the tank pressure at 0.7kPa, the stirring rate at 100rpm / min, and the ventilation rate at 1:0.5v / v.m, fermentation time 100 hours. Transfer the primary seed tank strains into a 1500L secondary seed culture tank with 800L seed culture medium, keep the tank temperature at 2...

Embodiment 2

[0034] Inoculate the mycelia of Leucobacterium in the slant culture medium, the culture temperature is 28.5°C, and the culture time is 168 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaker at 28.5°C for 96 hours, and the shaker rotated at 150rpm / min. Then the obtained shake flask strains were transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, cultured on a shaker at 28.5°C for 56 hours, and the shaker speed was 150rpm / min. Then transfer 2500ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 60L seed culture medium, keep the tank temperature at 28.5°C, the tank pressure at 0.7kPa, the stirring rate at 110rpm / min, and the ventilation rate at 1:0.5v / v.m, fermentation time 98 hours. Transfer the primary seed tank bacteria into a 1500L secondary seed culture tank with 800L fermentation medium, keep the tank temperature...

Embodiment 3

[0042] Inoculate the mycelium of Leucobacterium in the slant culture medium, the culture temperature is 29°C, and the culture time is 160 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaking table at 29°C for 96 hours, and the rotating speed of the shaking table was 150rpm / min. Then the obtained shake flask bacterial strain was transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, and cultured on a shaker at 29° C. for 44 hours at a shaker speed of 150 rpm / min. Then transfer 2500ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 50L seed culture medium, keep the tank temperature at 29°C, the tank pressure at 0.7kPa, the stirring rate at 100rpm / min, and the ventilation rate at 1:0.5v / v.m, fermentation time 96 hours. Transfer the primary seed tank bacteria into a 1000L secondary seed culture tank with 500L fermentation m...

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Abstract

A technology for preparing the mycelia, powder and polyose of armillarisiae by large-scale deep liquid fermenting includes such steps as shak-flask liquid culture, enlarge culture, stage-one seed culture, stage-two seed culture, stage-three fermenting culture to obtain mycelia in fermented liquid, high-speed stirring, concentrating, sterilizing and spray drying to obtain powder, or heating fermented liquid to 90-100 deg.C while stirring, and press filter to obtain polyose.

Description

technical field [0001] The invention relates to a large-scale liquid submerged fermentation process for producing bright fungus mycelium powder and its polysaccharide. technical background [0002] Bright fungus (Armillariella tabescens (Scop.: Fr.) Sing), English name Ringles HoneyMushroom, also known as luminous Armillariella tabescens, perishable cup umbrella, belongs to the order of Mushrooms, Tricholomaceae, Armillariella genus. The cap is oblate and hemispherical, gradually flattening, the middle part is concave, 2-7 cm in diameter, the surface is honey yellow to dark eggshell color, and the surrounding is slightly lighter than the middle part, with denser light rotten leaf color and dark brown hairs Small scales with complete edges and inconspicuous stripes; gills are light flesh-colored, slightly thinner, of different lengths, and nearly elongated with the stipe; the flesh is white to white-yellow, thick in the middle, thin at the edge, light and delicious. Stipe cy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 敖宗华符慧子邹锡良
Owner 敖宗华
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