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Temilmyces fuliginosus large-scale liquid deep fermentation production process

A technology of liquid submerged fermentation and production process, applied in the directions of horticulture, botanical equipment and methods, fungi, etc., can solve the problems such as large-scale liquid submerged fermentation production of chicken fir and the artificial cultivation of fruit body of chicken fir has not been successful yet.

Inactive Publication Date: 2005-09-28
敖宗华 +2
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AI Technical Summary

Problems solved by technology

At present, the artificial cultivation of the fruiting body of Jizheng fir has not been successful
There is no report on the large-scale liquid submerged fermentation production of chicken fir

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0022] Inoculate the mycelia of fir fir in the slant culture medium, the culture temperature is 28° C., and the culture time is 120 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaking table at 28°C for 48 hours, and the rotating speed of the shaking table was 150rpm / min. Then the obtained shake flask bacterial strain was transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, and cultured on a shaker at 28° C. for 24 hours at a shaker speed of 150 rpm / min. Then transfer 2200ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 50L seed medium, keep the tank temperature at 28°C, the tank pressure at 0.7kPa, the stirring rate at 100rpm / min, and the ventilation rate at 1:0.5v / v.m, fermentation time 45 hours. Transfer the primary seed tank strains into a 1500L fermenter with 800L fermentation medium, keep the tank temperature ...

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PUM

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Abstract

The present invention is aimed at providing a large-scale liquid deep bed fermentation process to produce termitophile mycelium. Said invention uses termitophile fungus from Jinxiang Inst. of Fungi, Shandong Prov. of China as initial strain, adopts slope to make liquid shave-flask cultivation, liquid shake-flask extension cultivation, first-stage seed cultivation and fermentation cultivation, finally, make the fermentation tank volume be up to 1000-5000 L. The utilization of said invented fermentation process makes fermentation in 1500L fermentation tank for 92 hr., the mycelium in the fermented liquor (by dried weight) can be up to 4.6%.

Description

technical field [0001] The invention relates to a large-scale liquid submerged fermentation production process of fir fir. technical background [0002] The formal name used at school is Termitomyces albuminosus (Berk.) Heim. It is a precious wild edible fungus that grows in termite nests in summer and autumn, and belongs to the genus Amanitaceae (Amanitaceae). Known as Jizong in ancient times, it is also known as umbrella mushroom (Sichuan), chicken shredded mushroom (Taiwan, Fujian), termite mushroom and so on. There is a close relationship between the growth and development of P. collusus and the activity of soil termites. Once the termites abandon the nest and go to him, the nest will no longer grow chicken fir fungus. Gallinarum is distributed in the tropical regions of Africa, Asia, the South Pacific Islands and the subtropical regions of Asia. It is also distributed in Jiangsu, Fujian, Taiwan, Guangdong, Guangxi, Sichuan, Guizhou, and Yunnan in China. [0003] Gall...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C12N1/14
Inventor 敖宗华符慧子邹锡良
Owner 敖宗华
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