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Large scale liquid deep fermentation for producing brilliant bacterial mycellium powder and its polysaccbaride technology

A liquid submerged fermentation and production process technology, applied in horticulture, application, botany equipment and methods, etc., can solve the problems of low production efficiency, easy to be infected with bacteria, long production cycle, etc.

Inactive Publication Date: 2006-10-25
敖宗华 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the bright bacteria preparations on the market are produced by the solid culture method of bright bacteria, which has the disadvantages of long production cycle, low production efficiency and easy contamination.
At present, there is no report on the large-scale production of Leucobacterium mycelium powder and its polysaccharides by using a three-stage liquid fermentation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Inoculate the mycelium of Leucobacterium in the slant culture medium, the culture temperature is 28°C, and the culture time is 180 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaker at 28°C for 100 hours, and the shaker speed was 150rpm / min. Then the obtained shake flask bacterial strain was transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, and cultured on a shaker at 28° C. for 48 hours at a shaker speed of 150 rpm / min. Then transfer 2200ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 50L seed medium, keep the tank temperature at 28°C, the tank pressure at 0.7kPa, the stirring rate at 100rpm / min, and the ventilation rate at 1:0.5v / v.m, fermentation time 100 hours. Transfer the primary seed tank strains into a 1500L secondary seed culture tank with 800L seed culture medium, keep the tank temperature at 2...

Embodiment 2

[0034] Inoculate the mycelia of Leucobacterium in the slant culture medium, the culture temperature is 28.5°C, and the culture time is 168 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaker at 28.5°C for 96 hours, and the shaker rotated at 150rpm / min. Then the obtained shake flask strains were transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, cultured on a shaker at 28.5°C for 56 hours, and the shaker speed was 150rpm / min. Then transfer 2500ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 60L seed culture medium, keep the tank temperature at 28.5°C, the tank pressure at 0.7kPa, the stirring rate at 110rpm / min, and the ventilation rate at 1:0.5v / v.m, fermentation time 98 hours. Transfer the primary seed tank bacteria into a 1500L secondary seed culture tank with 800L fermentation medium, keep the tank temperature...

Embodiment 3

[0042] Inoculate the mycelium of Leucobacterium in the slant culture medium, the culture temperature is 29°C, and the culture time is 160 hours. The fresh slant strains were inserted into a 500ml Erlenmeyer flask containing 100ml of culture medium, cultured on a shaking table at 29°C for 96 hours, and the rotating speed of the shaking table was 150rpm / min. Then the obtained shake flask bacterial strain was transferred into a 500ml Erlenmeyer shake flask equipped with 100ml medium for expansion culture, and cultured on a shaker at 29° C. for 44 hours at a shaker speed of 150 rpm / min. Then transfer 2500ml of the strains enlarged in the triangular flask into a 100L primary seed tank with 50L seed culture medium, keep the tank temperature at 29°C, the tank pressure at 0.7kPa, the stirring rate at 100rpm / min, and the ventilation rate at 1:0.5v / v.m, fermentation time 96 hours. Transfer the primary seed tank bacteria into a 1000L secondary seed culture tank with 500L fermentation m...

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PUM

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Abstract

Large-scale liquid submerged fermentation to produce bright fungus mycelium powder and its polysaccharide technology. The present invention aims at large-scale liquid submerged fermentation to produce bright fungus mycelium powder and its polysaccharides, takes bright fungus of Shandong Jinxiang Institute of Mycology as the starting strain, and adopts slanted strains to carry out liquid shake flask cultivation and liquid shake flask expansion Cultivation, primary seed cultivation, secondary seed cultivation and tertiary fermentation cultivation, the final volume of the fermentation tank reaches 10000L-15000L. Utilizing the fermentation process, fermented in a 10000L-15000L fermenter, the mycelium in the fermented liquid can reach 2-3% by dry weight, and the polysaccharide content in the dry bacteria powder can reach 12-18%. If it is necessary to obtain its bacterial powder, the fermented liquid is crushed at a high speed in a fermenter, concentrated and sterilized, and then aseptically spray-dried to obtain the bacterial powder. If it is necessary to obtain its polysaccharides, heat the temperature of the final fermentation broth to 90-100°C, stir at a speed of 200-250rpm / min, keep it for 2-4 hours, use a pump to transport the fermentation broth to a storage tank, and filter it through a plate and frame Obtain leucopolysaccharide.

Description

technical field [0001] The invention relates to a large-scale liquid submerged fermentation process for producing bright fungus mycelium powder and its polysaccharide. technical background [0002] Bright fungus (Armillariella tabescens (Scop.: Fr.) Sing), English name Ringles HoneyMushroom, also known as luminous Armillariella tabescens, perishable cup umbrella, belongs to the order of Mushrooms, Tricholomaceae, Armillariella genus. The cap is oblate and hemispherical, gradually flattening, the middle part is concave, 2-7 cm in diameter, the surface is honey yellow to dark eggshell color, and the surrounding is slightly lighter than the middle part, with denser light rotten leaf color and dark brown hairs Small scales with complete edges and inconspicuous stripes; gills are light flesh-colored, slightly thinner, of different lengths, and nearly elongated with the stipe; the flesh is white to white-yellow, thick in the middle, thin at the edge, light and delicious. Stipe cy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
Inventor 敖宗华符慧子邹锡良
Owner 敖宗华
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