Lipasegenous bactenia, its screening method and industrial application

Abstract

The present invention relates to a lipasegenic bacterium, its screening method and its industrial application. Said strain is a rhizopchin obtained by separating Chinese daqu liquor, its name is Rhizopus chinensis CCTCC M201021. Its screening method includes the following steps: taking daqu liquor sample or unstrained spirits, diluting and coating on plate, slant culture, shake-flask culture, centrifugal separation, injecting supernatant fluid into preliminary screen plate hole, selecting bacterium with larger transparent ring and / or fluorescent ring to make shake-flask fermentation and rescreening, freeze-drying thallus so as to obtian whole cell rhizopchin lipase enzyme preparation. It can be used for conversion synthesis of aromatic ester in orgnaic phase.

Description

technical field [0001] The invention relates to a lipase-producing bacterium and its screening method and industrial application, and belongs to the technical field of synthesizing aromatic esters in the organic phase of lipase in the food industry. Background technique [0002] Aromatic esters are a kind of short-chain fatty acid esters with natural fruity aroma. They are important components of flavors and fragrances and are widely used in daily necessities such as brewing, food, beverage, medicine and cosmetics. In my country, the annual demand for ethyl caproate for the beverage industry alone reaches about 3,000 tons. At present, the production of aromatic esters in the world, except for a very small number of esters which are extracted from natural plants, is all produced through traditional chemical synthesis methods. It is difficult to guarantee the purity and safety of the products, and some of them have brought many negative impacts on the environment. Although th...

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Problems solved by technology

At present, the production of aromatic esters in the world, except for a very small number of esters that are extracted from natural plants, is all produced by traditional chemical synthesis methods. It is difficult to guarantee the purity and safety of the products, and some of them have brought many negative impacts on the environment.

Although the chemical synthesis of fatty acid esters is relatively economical at present, with the pursuit of green food and the favor of natural products, direct extraction from plants cannot meet the growing demand, and people began to turn their attention to biotransformation methods to produce

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