Modified xanthan gum nano-micelle preparation method

A technology of nano-micelle and nano-micelle solution, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc. It can improve the drug release time, enhance the antioxidant performance, and be less prone to oxidative degradation.

Active Publication Date: 2017-11-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the side chain and main chain of xanthan gum form a helix and multiple helical advanced structures through hydrogen bonds, the dissolution rate of xanthan gum in

Method used

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  • Modified xanthan gum nano-micelle preparation method
  • Modified xanthan gum nano-micelle preparation method
  • Modified xanthan gum nano-micelle preparation method

Examples

Experimental program
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Example Embodiment

[0031] Example 1

[0032] 1-1) Synthesis of amphiphilic modified xanthan gum polymer modified by phthalic anhydride:

[0033] Dissolve 2g of xanthan gum in 200mL of deionized water, stir it evenly, add 0.06g of 4-dimethylaminopyridine, and slowly add 20mL of acetone solution with 0.5g of phthalic anhydride to the xanthan gum solution. Stir evenly, heat to 60℃ for 12h, then cool to room temperature, precipitate with 3 times the volume of ethanol, filter, wash the precipitate with ethanol three times, and vacuum dry at 60℃ to constant weight to obtain phthalic anhydride modified xanthogen Glue crude product; dissolve the crude product with deionized water, configure it as a 0.5w% solution, and transfer it to a dialysis bag with a molecular weight cut-off of 14000. The deionized water is the external dialysis fluid. The external dialysis fluid is changed every 5 hours. In less than 72 hours, remove unreacted small molecules and catalysts, freeze-dry the dialysis sample to obtain amph...

Example Embodiment

[0038] Example 2

[0039] As shown in Example 1-1), an aqueous solution of xanthan gum was prepared, the same amount of catalyst was added, and 20 mL of acetone solution dissolved in 1.0 g of phthalic anhydride was slowly added dropwise to the xanthan gum solution, stirred evenly, and heated After reacting at 60°C for 12 hours and cooling to room temperature, the post-treatment is as in Example 1-1), the modified xanthan gum nanomicelles are prepared as in Example 1-2), and the drug-loaded modified xanthan gum nanomicelles are prepared as in the implementation Example 1-3), the obtained modified xanthan gum nanomicelles are denoted as XP2.

Example Embodiment

[0040] Example 3

[0041] As shown in Example 1-1), an aqueous solution of xanthan gum was prepared, the same amount of catalyst was added, and 20 mL of acetone solution dissolved in 2.0 g of phthalic anhydride was slowly added dropwise to the xanthan gum solution, stirred evenly, and heated After reacting at 60°C for 12 hours and cooling to room temperature, the post-treatment is as in Example 1-1), the modified xanthan gum nanomicelles are prepared as in Example 1-2), and the drug-loaded modified xanthan gum nanomicelles are prepared as in the implementation Example 1-3), the obtained modified xanthan gum nanomicelles are denoted as XP3.

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Abstract

The invention relates to the technical field of biological and medical materials, in particular to a preparation method of injection type modified xanthan gum nano-micelle. The preparation method comprises the steps: performing esterification reaction on phthalic anhydride and xanthan gum, preparing amphipathic modified xanthan gum polymer by a one-step method and then self assembling and preparing modified xanthan nano-micelle by a selective solvent method. The xanthan gum is anhydride modified, so that biocompatibility and oxidation resistance of the xanthan gum are improved. The xanthan gum nano-micelle has a hydrophobic cavity with a benzene ring and a hydrophilic outer layer of the xanthan gum, so that circulation stability of the nano-micelle in a biological body is improved; in addition, the modified xanthan gum contains carboxylic groups, so that the nano-micelle has certain pH response; a charge effect of the nano-micelle can also be utilized to load medicine doxorubicin, so that the medicine loaded nano-micelle has a certain slow-release function. The preparation method disclosed by the invention has simple synthetic steps and is green, and the nano-micelle can be completely degraded in the body and hopefully serves as a carrier of anti-cancer drug to control release.

Description

technical field [0001] The invention relates to a preparation method of modified xanthan gum nano micelles, which belongs to the application of natural polymer materials in the field of biomedical technology. Background technique [0002] As a natural polysaccharide, xanthan gum is an exopolysaccharide secreted by Xanthomonas campestris, and is an important biopolymer. Its main chain structure is the same as that of cellulose, and its side chain terminal manna The sugar has a pyruvate group attached to it. Because the side chain and the main chain of xanthan gum form a helix and multiple helix advanced structure through hydrogen bonds, the dissolution rate of xanthan gum in water decreases, and the water solubility decreases. Some active groups are wrapped in the structure, which limits the xanthan gum. Antioxidant activity and stability. Because xanthan gum is non-toxic, harmless, and highly adaptable, it has a wide range of applications in the field of life sciences, suc...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K47/36A61K31/704A61P35/00
CPCA61K9/1075A61K31/704A61K47/36
Inventor 倪才华徐杰张丽萍石刚桑欣欣
Owner JIANGNAN UNIV
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