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Acclimatization and screening method for heterotrophic nitrification aerobic denitrifying bacteria

An aerobic denitrification and heterotrophic nitrification technology is applied in the field of domestication and screening of heterotrophic nitrification aerobic denitrification bacteria, which can solve the problems of large randomness, increased screening workload, and no more reliable screening methods, etc. The effect of great application prospects, saving workload, and strong ability to adapt to the environment

Active Publication Date: 2015-06-03
BLUESTAR LEHIGH ENG INST CO LTD
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Problems solved by technology

[0005] The Chinese published patent document CN 102041291 B discloses a screening method for denitrifying bacteria. The method is to separate and purify the denitrifying bacteria after enrichment, and to domesticate the growth ability of the isolated strains by different methods. Each strain of the separated and purified strains is subjected to The domestication of growth ability has increased the workload of screening; Chinese published patent document CN1594591A discloses a method for screening aerobic denitrifying bacteria. Aerobic denitrifying bacteria were obtained by re-screening, but the total nitrogen removal ability of the bacteria screened by this method is insufficient
However, the screening of heterotrophic nitrifying and aerobic denitrifying functional strains is relatively random, and there is no reliable screening method.

Method used

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Embodiment 1

[0026]Embodiment 1, a method for acclimation and screening of heterotrophic nitrification and aerobic denitrification bacteria, comprising the following steps:

[0027] (1) Inoculate the sample into the enriched nutrient solution containing nitrogen-containing wastewater to carry out shake-flask heterotrophic enrichment culture for the denitrification bacteria group to obtain the enrichment culture solution for the denitrification bacteria group;

[0028] (2) Use nitrogen-containing wastewater in an aerobic aeration reactor to rapidly proliferate the denitrification bacteria in the enriched culture solution;

[0029] (3) Use the actual nitrogen-containing wastewater to domesticate and cultivate the heterotrophic denitrification bacteria in the SBR reactor;

[0030] (4) Use the A / O process and the actual nitrogen-containing wastewater to domesticate and cultivate the denitrification bacteria;

[0031] (5) Separation and purification of denitrification bacteria from domesticate...

Embodiment 2

[0034] Example 2, in step (1) of the method described in Example 1: the inoculated sample is one or more of soil samples, activated sludge, and sewage; the composition of each L of enriched nutrient solution is: (NH 4 ) 2 SO 4 0.5~1g / L, glucose 0.5~1g / L, KH 2 PO 4 or K 2 HPO 4 0.5~5.0 g / L, MgCl 2 0.5~5.0 g / L, Na 2 CO 3 0.5~5.0 g / L, nitrogen-containing waste water 50mL, the rest is distilled water; the conditions of shake flask heterotrophic enrichment culture are: temperature: 10℃~35℃, pH: 6.0~10.0, speed: 150rpm~220rpm; During the collection culture process, when the nitrogen degradation reaches more than 90%, the enriched culture solution is transferred to the fresh enriched nutrient solution at a volume of 5-10%, and the number of viable bacteria is ≥2×10 9 The culture was terminated above.

Embodiment 3

[0035] Example 3, in step (1) of the method described in Example 1: the inoculated sample is one of soil sample, activated sludge, and sewage; the composition of each L enriched nutrient solution is: (NH 4 ) 2 SO 4 0.5g / L, glucose 0.5g / L, KH 2 PO 4 or K 2 HPO 4 0.5 g / L, MgCl 2 0.5 g / L, Na 2 CO 3 0.5g / L, nitrogen-containing waste water 40mL, the rest is distilled water; the conditions of shake flask heterotrophic enrichment culture are: temperature: 10°C, pH: 6.0, rotation speed: 150rpm.

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Abstract

The invention discloses an acclimatization and screening method for heterotrophic nitrification aerobic denitrifying bacteria. According to the invention, the heterotrophic nitrification aerobic denitrifying bacteria are obtained through shaking flask enrichment, sludge accumulation, SBR reactor batch acclimation and low-DO (Dissolved Oxygen) process gradual acclimation and isolation and screening, and a method for efficiently acquiring target strains is established. According to the screening method of the invention, the heterotrophic nitrification aerobic denitrifying bacteria can be successfully screened, further, microbial preparation prepared with the screened bacteria as active components can be used for effectively depriving ammonia nitrogen and total nitrogen in the water, meanwhile, and the preparation has tolerance and degradation ability for phenols, amines, heterocyclic materials, cyanogens and polyaromatic hydrocarbons and other toxic materials in waste water, has strong accommodative ability and has great application prospect in actual engineering application.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a domestication and screening method for heterotrophic nitrification and aerobic denitrification bacteria. Background technique [0002] The traditional wastewater biological denitrification technology mainly relies on autotrophic nitrifying bacteria and heterotrophic denitrifying bacteria to convert ammonia nitrogen into nitrogen gas through nitrification and denitrification coupling. However, autotrophic bacteria have a slow proliferation rate, cannot compete with heterotrophic bacteria in the mixed culture activated sludge system, are difficult to obtain high biomass, and have low nitrification efficiency, resulting in weak impact resistance of the autotrophic microbial denitrification system. Nitrification is incomplete; denitrifying bacteria are heterotrophic bacteria, and carbon sources often need to be added during operation, which increase...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/20C12N1/02
CPCC12N1/02C12N1/20C12N1/36
Inventor 田凤蓉王开春张彬彬杨志林云干李坤郭涛陈添敏崔庆兰
Owner BLUESTAR LEHIGH ENG INST CO LTD
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