Artificial cultivation method for Taiwan cordyceps fruiting bodies

A technology of Cordyceps fruiting body and artificial cultivation, applied in the field of artificial cultivation of medicinal fungi, can solve the problem of lack of wild Taiwan Cordyceps resources, etc., and achieve the effects of convenient commercial sales, fast growth and simple production process

Inactive Publication Date: 2011-08-03
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no reports and patents on the method of cultivating the fruiting body of Cordyceps taiwanensis at home and abroad. In addition, there are few wild resources o

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] C. huangshanensis Hirsutella huangshanensis The strains can be obtained by collecting mature Cordyceps taiwanensis in the field, collecting the ejected ascospores, transferring them to a sterile culture medium for separation and purification, or separating and purifying the sporozoites using tissue separation after surface disinfection. kind. Huangshan Mountain used in the present invention Hirsutella huangshanensis The strains are preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC No 4147.

[0033] The specific production operation steps of the method for artificially cultivating the fruiting body of Cordyceps taiwanensis are as follows:

[0034] A. Activation and cultivation of strains in inclined test tubes

[0035] Trichoderma huangshanense Hirsutella huangshanensis The strains were inoculated in the slant test tube culture medium, cultivated at 25°C for 10 days, and ...

Embodiment 2

[0048] C. huangshanensis Hirsutella huangshanensis The strains can be obtained by collecting mature Cordyceps taiwanensis in the field, collecting the ejected ascospores, transferring them to a sterile culture medium for separation and purification, or separating and purifying the sporozoites using tissue separation after surface disinfection. kind. Huangshan Mountain used in the present invention Hirsutella huangshanensis The strains are preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC No 4147.

[0049] The specific production operation steps of the method for artificially cultivating the fruiting body of Cordyceps taiwanensis are as follows:

[0050] A. Activation and cultivation of strains in inclined test tubes

[0051] Trichoderma huangshanense Hirsutella huangshanensis The strains were inoculated in the slant test tube culture medium, cultivated at 25°C for 10 days, and ...

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PUM

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Abstract

The invention relates to an artificial cultivation method for Taiwan cordyceps fruiting bodies. Hirsutellahuangshanensis is adopted as a strain. The detailed cultivation operation comprises the following steps: A, activating and cultivating the strain in a test tube with an oblique surface; B, cultivating seeds in a shake flask filled with liquid; C, cultivating mycelia; D, and cultivating fruiting bodies to obtain artificially cultivated Taiwan cordyceps fruiting bodies which are rod-shaped and are orange yellow or orange red in color, wherein the length and the dimension of the artificially cultivated Taiwan cordyceps fruiting bodies are similar to the length and the dimension of wild Taiwan cordyceps fruiting bodies. The method is initiative; the Taiwan cordyceps fruiting bodies can be smoothly cultivated by using the method; the problem of the lack of wild Taiwan cordyceps resources is solved; and the method provides a broad prospect for comprehensive development, industrial production, medical use, edible use and further analysis, extraction and separation of active components of Taiwan cordyceps; moreover, the method has the advantages of adopting simple production process, realizing low cost, having wide sources of raw materials, leading to no pollution and being suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation methods of medicinal fungi, in particular to the use of Mortierella huangshanense species ( Hirsutella huangshanensis ) for slant culture, shake flask culture and solid culture to form the fruiting body of Cordyceps taiwanensis. Background technique [0002] Fungi of the genus Cordyceps are a large class of important entomopathogenic fungi, which mainly parasitize on the fruiting bodies of some underground species of insects, spiders and some Elaphomyces Nees & Fr. Most of them can infect insects, and grow sporosomes from their heads or body surfaces to form a complex of insects and bacteria, that is, Cordyceps. The fungus of the genus Cordyceps infects the host insect to make it ossified, and grows the sporosome from the infected zombie under suitable conditions, thus forming a fruiting body structure with a specific shape of the insect (zombie) grass (fungi sporoplex) complex. ...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 李春如樊美珍董建飞
Owner ANHUI AGRICULTURAL UNIVERSITY
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