Non-invasive biomass online detection device used for shake-flask culture

Abstract

The invention discloses a non-invasive biomass online detection device used for shake-flask culture. The device comprises a master control unit, a shake flask capable of containing biological turbid liquid and a fixing sleeve covering the outer side of the shake flask. A detection probe is connected to the fixing sleeve on the outer side of the bottom of the shake flask. The detection probe comprises a substrate capable of being attached to the bottom of the shake flask, a light source, a photoelectric sensor and an acceleration sensor, wherein the light source, the photoelectric sensor and the acceleration sensor are arranged on the substrate. The light source can generate a light path obliquely irradiated into the shake flask. The photoelectric sensor is located below the light path and used for measuring scattering light generated after the light path is irradiated to the biological turbid liquid. The light source, the photoelectric sensor and the acceleration sensor are all connected with the master control unit. According to the non-invasive biomass online detection device, a turbidity measurement method is adopted for performing online detection on the concentration of bacterial liquid cultured in the shake flask through a back scattering light measurement technology, and the concentration of the biological turbid liquid in the shake flask can be detected under the condition that culture equipment is not broken. The non-invasive biomass online detection device is simple in structure, low in cost, and capable of effectively improving detection efficiency and precision and avoiding sample contamination.

Description

technical field [0001] The invention is used in the technical field of cell or bacterium culture, and in particular relates to a non-invasive biomass online detection device for shake flask culture. Background technique [0002] Shake flasks are widely used in microbial cell culture due to their easy handling, low cost, and simple setup. In the biological laboratory, the monitoring of the growth of shake flask culture bacteria often adopts multiple sampling measurements to obtain its optical density (OD), dry cell weight (CDW) or cell concentration. Since the whole process of cell culture cannot be automatically monitored, the culture of each cell usually needs to measure the offline value several times until the concentration reaches the requirement, and then conduct a series of follow-up experiments. Taking the commonly used optical density (absorbance) method as an example, with the continuous increase of the concentration of the suspension, the linear range of the spect...

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Problems solved by technology

At the same time, the shaker flask or Erlenmeyer flask used in the shaker does not have a special sample outlet, and each sampling increases the risk of infection with miscellaneous bacteria

If an experimenter moni

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