Secretions of marine halomonas and extraction method and application of secretions

Abstract

The invention relates to secretions of marine halomonas, an extraction method of the secretions and the application of the secretions. The extraction method comprises the following steps: preparing a culture medium according to a formula of 5 g of yeast powder, 10 g of tryptone and 1L of seawater, and sterilizing the culture medium for 15-20 minutes at the temperature of 121 DEG C; inoculating the marine halomonas into the culture medium under aseptic conditions, culturing the culture medium inoculated with the marine halomonas for 10-15 days at the constant temperature of 25-30 DEG C on a backward rotating shaking table at the speed of 120-150 rpm, performing filtration with a film of 0.22-0.45 micrometers, and performing sterilization so as to obtain bacterial exudate supernatant; vacuum freezing and drying a filtrate, performing extraction through a pure acetic acid, and performing filtration and desalination through filter paper; volatilizing the acetic acid of the obtained filtrate so as to obtain coarse substances of the secretions of the marine halomonas. The secretions disclosed by the invention can be used for governing red tides, and have the effect of restraining dinoflagellates like Alexandrium tamarenses, prorocentrum donghaiense and Karenia mikimotoi of the red tides. According to the invention, the culture conditions are simple, products do not need to be refined, only the filtration is needed for removing thalli, and the secretions have a good effect of restraining the dinoflagellates of the red tides.

Description

technical field

[0001] The present invention relates to the secretion of marine halomonas and its extraction method and application. Background technique

[0002] In recent years, there have been frequent outbreaks of dinoflagellate blooms in the ocean, and most red tide dinoflagellates produce toxins, such as hemolysin, paralytic shellfish poisoning (PSP), brevetoxin- group, BTX), not only the red tide itself has serious consequences on the marine ecological environment, but also these toxins produced by algae will cause great harm to marine animals such as fish and shellfish, and even threaten people's health, so they are highly valued by coastal countries . Judging from the existing conditions, once a large area of ​​red tide occurs, there is no particularly effective method to stop it. For some local and small-scale methods of preventing and controlling red tide, although many experiments have been done, the effect is not ideal enough. Mainly use chemical drugs (copper...

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