Salt-tolerant efficient denitrification-type microbial preparation for water treatment
A technology for microbial preparation and water treatment, applied in microorganisms, biological water/sewage treatment, microorganism-based methods, etc., can solve the problems of toxic microorganisms, low treatment efficiency, loss of degradation ability, etc., achieve good adsorption performance, improve recovery Effect, effect of good denitrification ability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0027] Bacteria powder is mixed according to the mass ratio of halophilic archaea 5%, Bacillus subtilis 5%, Pseudomonas denitrification 5%, Halomonas 5% and halophilic bacteria 5%, adding 5% (w / t) bamboo charcoal particles.
[0028] The preparation method is as follows: adding the halophilic archaea into the basic medium LB culture solution. Place them in a constant temperature shaking shaker at 30°C and 180r / min for 12 hours of activation, then transfer them to a fermenter containing denitrification medium at an inoculum size of 5% for expanded cultivation, and stop the fermentation after 48 hours of fermentation. Collect the fermentation broth, centrifuge at 6000r / min at low temperature for 10min, take the precipitate and resuspend it with sterile phosphate buffer to obtain the target bacterial suspension, and finally freeze-dry the bacterial suspension to obtain bacterial powder. The same method is used to obtain Pseudomonas denitrifica, Bacillus subtilis, nitrifying bacte...
Embodiment 2
[0030] The bacteria powder is mixed according to the mass ratio of halophilic archaea 7.5%, Bacillus subtilis 7.5%, Pseudomonas denitrification 7.5%, Halomonas 7.5% and halophilic bacteria 7.5%, adding 7.5% (w / t ) of bamboo charcoal particles.
[0031] The preparation method is as follows: adding the halophilic archaea into the basic medium LB culture solution. Place them in a constant temperature shaking shaker at 30°C and 180r / min for 12 hours of activation, then transfer them to a fermenter containing denitrification medium at an inoculum size of 5% for expanded cultivation, and stop the fermentation after 48 hours of fermentation. Collect the fermentation broth, centrifuge at 6000r / min at low temperature for 10min, take the precipitate and resuspend it with sterile phosphate buffer to obtain the target bacterial suspension, and finally freeze-dry the bacterial suspension to obtain bacterial powder. The same method is used to obtain Pseudomonas denitrifica, Bacillus subtil...
Embodiment 3
[0033] Bacteria powder is mixed according to the mass ratio of halophilic archaea 10%, Bacillus subtilis 10%, Pseudomonas denitrification 10%, Halomonas 10% and halophilic bacteria 10%, adding 10% (w / t) bamboo charcoal particles.
[0034] The preparation method is as follows: adding the halophilic archaea into the basic medium LB culture solution. Place them in a constant temperature shaking shaker at 30°C and 180r / min for 12 hours of activation, then transfer them to a fermenter containing denitrification medium at an inoculum size of 5% for expanded cultivation, and stop the fermentation after 48 hours of fermentation. Collect the fermentation broth, centrifuge at 6000r / min at low temperature for 10min, take the precipitate and resuspend it with sterile phosphate buffer to obtain the target bacterial suspension, and finally freeze-dry the bacterial suspension to obtain bacterial powder. The same method is used to obtain Pseudomonas denitrifica, Bacillus subtilis, nitrifying...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More - R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com