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Salt-tolerant efficient denitrification-type microbial preparation for water treatment

A technology for microbial preparation and water treatment, applied in microorganisms, biological water/sewage treatment, microorganism-based methods, etc., can solve the problems of toxic microorganisms, low treatment efficiency, loss of degradation ability, etc., achieve good adsorption performance, improve recovery Effect, effect of good denitrification ability

Inactive Publication Date: 2019-09-17
天津金辰博科环保科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high concentration of inorganic salts in high-salt wastewater will inhibit or even poison the microorganisms in activated sludge, destroy its flora structure, and cause it to lose its degradation ability. Therefore, the application of biochemical methods in the treatment of high-salt wastewater has been greatly restricted.
In recent years, people have gradually adapted to the salt environment of high-salt wastewater by acclimating ordinary activated sludge to obtain microorganisms with a certain salt tolerance, but there are still some microorganisms with low salt tolerance, low treatment efficiency, and poor resistance to salinity shocks And other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Bacteria powder is mixed according to the mass ratio of halophilic archaea 5%, Bacillus subtilis 5%, Pseudomonas denitrification 5%, Halomonas 5% and halophilic bacteria 5%, adding 5% (w / t) bamboo charcoal particles.

[0028] The preparation method is as follows: adding the halophilic archaea into the basic medium LB culture solution. Place them in a constant temperature shaking shaker at 30°C and 180r / min for 12 hours of activation, then transfer them to a fermenter containing denitrification medium at an inoculum size of 5% for expanded cultivation, and stop the fermentation after 48 hours of fermentation. Collect the fermentation broth, centrifuge at 6000r / min at low temperature for 10min, take the precipitate and resuspend it with sterile phosphate buffer to obtain the target bacterial suspension, and finally freeze-dry the bacterial suspension to obtain bacterial powder. The same method is used to obtain Pseudomonas denitrifica, Bacillus subtilis, nitrifying bacte...

Embodiment 2

[0030] The bacteria powder is mixed according to the mass ratio of halophilic archaea 7.5%, Bacillus subtilis 7.5%, Pseudomonas denitrification 7.5%, Halomonas 7.5% and halophilic bacteria 7.5%, adding 7.5% (w / t ) of bamboo charcoal particles.

[0031] The preparation method is as follows: adding the halophilic archaea into the basic medium LB culture solution. Place them in a constant temperature shaking shaker at 30°C and 180r / min for 12 hours of activation, then transfer them to a fermenter containing denitrification medium at an inoculum size of 5% for expanded cultivation, and stop the fermentation after 48 hours of fermentation. Collect the fermentation broth, centrifuge at 6000r / min at low temperature for 10min, take the precipitate and resuspend it with sterile phosphate buffer to obtain the target bacterial suspension, and finally freeze-dry the bacterial suspension to obtain bacterial powder. The same method is used to obtain Pseudomonas denitrifica, Bacillus subtil...

Embodiment 3

[0033] Bacteria powder is mixed according to the mass ratio of halophilic archaea 10%, Bacillus subtilis 10%, Pseudomonas denitrification 10%, Halomonas 10% and halophilic bacteria 10%, adding 10% (w / t) bamboo charcoal particles.

[0034] The preparation method is as follows: adding the halophilic archaea into the basic medium LB culture solution. Place them in a constant temperature shaking shaker at 30°C and 180r / min for 12 hours of activation, then transfer them to a fermenter containing denitrification medium at an inoculum size of 5% for expanded cultivation, and stop the fermentation after 48 hours of fermentation. Collect the fermentation broth, centrifuge at 6000r / min at low temperature for 10min, take the precipitate and resuspend it with sterile phosphate buffer to obtain the target bacterial suspension, and finally freeze-dry the bacterial suspension to obtain bacterial powder. The same method is used to obtain Pseudomonas denitrifica, Bacillus subtilis, nitrifying...

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Abstract

The invention discloses a salt-tolerant efficient denitrification-type microbial preparation for water treatment. The microbial preparation consists of the following components in percentage: 2.5-5% of halophilic archaea, 5-10% of bacillus subtilis, 5-10% of pseudomonas denitrificans, 5-10% of halomonas and 5-10% of halophilic bacteria, and 5-10% (w / t) of bamboo charcoal particles are added. The microbial preparation for water treatment consisting of halophilic archaea, pseudomonas denitrificans, bacillus subtilis, nitrifying bacteria, denitrifying bacteria and coryneform bacteria still has good denitrification capacity under the condition of high salt, and solves the limitation of a high salt environment on traditional biological denitrification methods. The bamboo charcoal particles have good adsorption performance because of large specific surface area, can be in direct contact with microorganisms better, and can adsorb organic matters on the surface, so that the formation of a surface biological membrane is promoted; and on the other hand, a large amount of oxygen can be stored in the bamboo charcoal particles, so that oxygen-containing components in a water area are improved, and a water area recovery effect is improved.

Description

technical field [0001] The invention belongs to the field of microbial preparation production, and in particular relates to a salt-tolerant and high-efficiency denitrification type water treatment microbial preparation. Background technique [0002] High-salt wastewater refers to wastewater with a salt content greater than 1% (w / v), and some even exceed 20%. It has a wide range of sources and complex components, and contains a large amount of soluble inorganic salt ions such as Cl-, SO42-, Na+ and Ca2+. At present, the treatment methods of high-salt wastewater mainly include physical and chemical methods, biochemical methods and combined processes. Among them, physical and chemical methods include evaporation, membrane separation, ion exchange electrolysis, etc. Although the treatment effect is good, most of them have problems such as high cost and high energy consumption, which seriously restricts the application of physical and chemical methods in the actual high-salt was...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/125C12R1/15
CPCC12N1/20C02F3/341
Inventor 曹秀坤
Owner 天津金辰博科环保科技发展有限公司
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