Salt-tolerant efficient denitrification-type microbial preparation for water treatment
A technology for microbial preparation and water treatment, applied in microorganisms, biological water/sewage treatment, microorganism-based methods, etc., can solve the problems of toxic microorganisms, low treatment efficiency, loss of degradation ability, etc., achieve good adsorption performance, improve recovery Effect, effect of good denitrification ability
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Embodiment 1
[0027] Bacteria powder is mixed according to the mass ratio of halophilic archaea 5%, Bacillus subtilis 5%, Pseudomonas denitrification 5%, Halomonas 5% and halophilic bacteria 5%, adding 5% (w / t) bamboo charcoal particles.
[0028] The preparation method is as follows: adding the halophilic archaea into the basic medium LB culture solution. Place them in a constant temperature shaking shaker at 30°C and 180r / min for 12 hours of activation, then transfer them to a fermenter containing denitrification medium at an inoculum size of 5% for expanded cultivation, and stop the fermentation after 48 hours of fermentation. Collect the fermentation broth, centrifuge at 6000r / min at low temperature for 10min, take the precipitate and resuspend it with sterile phosphate buffer to obtain the target bacterial suspension, and finally freeze-dry the bacterial suspension to obtain bacterial powder. The same method is used to obtain Pseudomonas denitrifica, Bacillus subtilis, nitrifying bacte...
Embodiment 2
[0030] The bacteria powder is mixed according to the mass ratio of halophilic archaea 7.5%, Bacillus subtilis 7.5%, Pseudomonas denitrification 7.5%, Halomonas 7.5% and halophilic bacteria 7.5%, adding 7.5% (w / t ) of bamboo charcoal particles.
[0031] The preparation method is as follows: adding the halophilic archaea into the basic medium LB culture solution. Place them in a constant temperature shaking shaker at 30°C and 180r / min for 12 hours of activation, then transfer them to a fermenter containing denitrification medium at an inoculum size of 5% for expanded cultivation, and stop the fermentation after 48 hours of fermentation. Collect the fermentation broth, centrifuge at 6000r / min at low temperature for 10min, take the precipitate and resuspend it with sterile phosphate buffer to obtain the target bacterial suspension, and finally freeze-dry the bacterial suspension to obtain bacterial powder. The same method is used to obtain Pseudomonas denitrifica, Bacillus subtil...
Embodiment 3
[0033] Bacteria powder is mixed according to the mass ratio of halophilic archaea 10%, Bacillus subtilis 10%, Pseudomonas denitrification 10%, Halomonas 10% and halophilic bacteria 10%, adding 10% (w / t) bamboo charcoal particles.
[0034] The preparation method is as follows: adding the halophilic archaea into the basic medium LB culture solution. Place them in a constant temperature shaking shaker at 30°C and 180r / min for 12 hours of activation, then transfer them to a fermenter containing denitrification medium at an inoculum size of 5% for expanded cultivation, and stop the fermentation after 48 hours of fermentation. Collect the fermentation broth, centrifuge at 6000r / min at low temperature for 10min, take the precipitate and resuspend it with sterile phosphate buffer to obtain the target bacterial suspension, and finally freeze-dry the bacterial suspension to obtain bacterial powder. The same method is used to obtain Pseudomonas denitrifica, Bacillus subtilis, nitrifying...
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