High-salinity nitrogen-containing wastewater stress resistance auxiliary nitrogen removal method

A wastewater and denitrification technology, applied in the field of microbial denitrification, can solve the problems of low denitrification efficiency and achieve broad application prospects

Active Publication Date: 2017-05-31
DALIAN MARITIME UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention solves the problem of low denitrification efficiency due to the inhibition of high salt on the growth

Method used

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  • High-salinity nitrogen-containing wastewater stress resistance auxiliary nitrogen removal method
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  • High-salinity nitrogen-containing wastewater stress resistance auxiliary nitrogen removal method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Synthesis and secretion experiment of Halomonas venusta DSM 4743 ectoine

[0034] Strain: Halomonas venusta DSM 4743

[0035] Strain activation: Medium A, 30°C, 120r / min, 24h.

[0036] Ectrahydropyrimidine induction culture: Inoculate the above-mentioned activated bacterial solution into medium B (30mL / 300mL) at an inoculation amount of 1%, at 30°C, 120r / min, for 48h.

[0037] Determination of ectoine concentration:

[0038] Intracellular ectoine: Take 1 mL of fermentation broth, centrifuge (4°C, 14000×g, 15 min), add 1 mL of 80% ethanol (v / v) to the pellet to resuspend, and let stand at room temperature overnight. The suspension was centrifuged again, and the supernatant was taken for HPLC determination.

[0039] Extracellular ectoine: Take 1 mL of fermentation broth, centrifuge (4°C, 14000×g, centrifuge for 15 min), take the supernatant, dilute 10 times with deionized water, and use it for HPLC determination.

[0040] The total concentration of ectoine i...

Embodiment 2

[0044] Example 2 Acinetobacter calcium acetate CCTCC AB 205304 and Pseudomonas maritima MCCC 1A02281 absorb ectoine experiment

[0045] In order to examine whether the SND denitrification strain Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas maritima MCCC1A02281 can absorb ectoine, the following experiments were carried out.

[0046] Strains: Acinetobacter calcoaceticus CCTCC AB 205304, Pseudomonas maritima MCCC 1A02281.

[0047] Strain activation: medium A (adjust NaCl concentration to 5g / L), 30°C, 120r / min, 24h.

[0048] Cell culture: Inoculate the above-mentioned activated strains into medium A (adjust the NaCl concentration to 5 g / L) at 1% inoculation amount, culture at 30° C., 120 r / min, for 48 hours.

[0049] Absorption of ectoine: Centrifuge the cell culture medium at 14000×g at 4°C for 15 min, discard the supernatant, and wash the cells twice with NaCl-KpiBuffer (100 mM, pH 7.2, NaCl 5 g / L). Then the thallus was transferred to 30mL NaCl-Kpi Buffer (100mM...

Embodiment 3 4

[0052] Example 3 Stress-resistance assisting experiment of ectoine-secreting strains on growth of SND denitrification strains at different salt concentrations

[0053] The ectoine-secreting strain (Halomonas venusta DSM 4743) and the SND denitrification strain (Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas marinum MCCC 1A02281) were mixed and cultured (containing 0, 15, 30, 45 , 60, 75, 90g / L NaCl medium C). In this combined culture system, the strain Halomonas venusta DSM4743 synthesized and secreted ectoine under the induction of NaCl, while Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas maritimus MCCC 1A02281 absorbed and secreted ectoine into the culture, and obtained Stress resistance, salt-tolerant growth. In the control experiment of salt-tolerant growth, the SND denitrification strain or the ectoine-secreting strain were cultured alone.

[0054] Strains: Halomonas venusta DSM 4743, Acinetobacter calcoaceticus CCTCC AB 205304, Pseudomonas ma...

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Abstract

The invention discloses a high-salinity nitrogen-containing wastewater stress resistance auxiliary nitrogen removal method, and belongs to the technical field of microorganism nitrogen removal. By constructing a tetrahydro-2-pyrimidinone secreting type halomonas meridian and traditional denitrifier strain combined stress resistance auxiliary synchronous heterotrophic nitrification aerobic denitrification nitrogen removal mode, the high-salinity nitrogen-containing wastewater nitrogen removal technology is provided. The problem that according to existing high-salinity nitrogen-containing wastewater nitrogen removal, the nitrogen removal efficiency is low due to inhibition of high salinity on denitrifier strain growth and metabolism is solved, and the application prospect on the aspects of high-salinity nitrogen-containing wastewater treatment and seawater aquatic water purification is achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial denitrification, and in particular relates to a denitrification method based on synchronous heterotrophic nitrification and aerobic denitrification assisted by microbial stress resistance. Background technique [0002] Ammonia nitrogen is one of the main pollutants in water bodies in my country. The unreasonable discharge of ammonia nitrogen has led to increasingly serious ammonia nitrogen pollution in natural water bodies such as rivers, lakes, groundwater, and coastal waters. Biological and environmental safety issues caused by ammonia nitrogen pollution have been highly concerned. The theoretical and technical research on ammonia nitrogen degradation and purification treatment of sewage and natural water environment has great economic value and social benefits. [0003] The technical methods that have been used for ammonia nitrogen purification mainly include physical and chemical methods s...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/38C12R1/05C12R1/085
CPCC02F3/34C12N1/20
Inventor 张苓花王特朱益民薛平常子民
Owner DALIAN MARITIME UNIVERSITY
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