Composition with light damage resistance as well as application and cosmetics thereof

Abstract

The invention belongs to the field of daily chemicals, and discloses a composition with light damage resistance, which comprises a ribonucleic acid solution, tetrahydromethylpyrimidine carboxylic acid and a spirulina maxima extract solution in a weight ratio of (60-80): (10-30): (1-10). The composition takes a ribonucleic acid solution, tetrahydromethylpyrimidinecarboxylic acid and a spirulina maxima extract solution as core components, can play a better anti-light damage effect, and samples are sent to a third-party detection mechanism for zebrafish embryo light damage cell apoptosis test. The test result shows that the effect of the composition is better than the cell apoptosis effect of ergothioneine with the same concentration, and evaluation of the skin moisture content increasing effect shows that the composition can well repair and supplement the skin barrier; meanwhile, the invention further discloses application of the composition and cosmetics.

Description

Technical Field

[0001] The present invention relates to the field of daily chemicals, and in particular to a composition with photodamage resistance, application thereof, and cosmetics. Background Art

[0002] Preventing skin aging has always been a major concern in skincare. Skin aging can be divided into two basic processes: intrinsic and extrinsic. Studies have shown that over 80% of extrinsic aging is caused by photoaging, characterized by severe wrinkles and pigment changes. Photoaging primarily refers to ultraviolet (UV), visible light, and infrared (IR). Currently, sunscreens are primarily focused on protecting against UV rays, particularly UVB and UVA, which have wavelengths between 280 and 400 nm.

[0003] Anti-photodamage cosmetics are a hot topic in the current market, as can be seen in the following literature:

[0004] The patent application with publication number CN111557875A is a skin care composition that resists light pollution and light damage, its preparation method and application. It discloses that ergothioneine, matsutake mushroom extract, ectoine (ectoine), nasturtium extract and hyaluronic acid substances can play a role in resisting light damage. It is further explained in the description that ectoine is a cell all-round protector and an excellent anti-stress protection molecule. It is a multifunctional active ingredient that has the function of stabilizing the protein hydration layer, protecting enzymes, DNA and other biological macromolecules and cell membrane structures, and can help cells and animals and plants resist various adverse environments such as freezing, drought, high temperature, high salt, and radiation.

[0005] The patent application with publication number CN118634174A, which is titled "A sunscreen synergistic composition with anti-blue light and light damage repair effects and its application", discloses that Spirulina MAXIMA extract contains photolyase, an enzyme that can repair DNA by absorbing blue-violet light, thereby reducing damage from ultraviolet rays.

[0006] During the research on Ectoin and Spirulina maxima extract, we found that the anti-photodamage effect of cosmetics can be further improved by combining the substances. Summary of the Invention

[0007] The present invention aims to provide a composition with resistance to light damage, which comprises a ribonucleic acid solution, ectoine, and a spirulina extract solution as core components, and has a good anti-light damage effect. The sample is sent to a third-party testing agency for a zebrafish embryo light-damaged cell apoptosis test, and the test results show that the effect of the present invention is better than the cell apoptosis effect of ergothioneine at the same concentration. At the same time, it can be seen from the evaluation of the skin moisture content improvement effect that the composition of the present invention can better repair and replenish the skin barrier.

[0008] At the same time, the invention also discloses the application of the composition and cosmetics.

[0009] To achieve the above objectives, this application discloses:

[0010] A composition with photodamage resistance, comprising a ribonucleic acid solution, ectoine, and a spirulina maxima extract solution, wherein the weight ratio of the ribonucleic acid solution, ectoine, and spirulina maxima extract solution is 60-80:10-30:1-10;

[0011] The concentration of ribonucleic acid in the ribonucleic acid solution is 3.5 wt%;

[0012] The concentration of the Spirulina maxima extract in the Spirulina maxima extract solution is 20 wt %.

[0013] In the present invention, the efficacy of the ribonucleic acid solution is as follows: the ribonucleic acid originating from the yeast cytoplasm is a small molecule active ingredient refined after biological enzymatic hydrolysis, which is more easily absorbed and utilized by the skin, promotes fibroblast proliferation, and stimulates cell vitality.

[0014] The efficacy of the Spirulina maxima extract solution is as follows: ultraviolet rays damage DNA by forming pyrimidine dimers (CPD) to cut off the nucleic acid chain; Spirulina maxima extract repairs DNA by directly decomposing CPD.

[0015] The combination of the above three exhibits a very obvious anti-photodamage effect, and the possible synergistic mechanism is:

[0016] Tetrahydropyrimidine carboxylic acid (TECA) blocks UVA-induced second messenger release and AP-2 activation, thereby reducing nuclear expression of pro-inflammatory factors such as intercellular adhesion molecule-1 (ICAM-1) and mitochondrial DNA mutations. It inhibits cellular aging in keratinocytes and fibroblasts, thereby counteracting UVA-induced and accelerated skin aging. Ectohydrogen methylpyrimidine carboxylic acid (TECA), derived from RNA in yeast cytoplasm and refined through enzymatic hydrolysis, is a small molecule active ingredient that is more readily absorbed by the skin, promoting fibroblast proliferation and stimulating cell viability. UV damage to DNA occurs by forming pyrimidine dimers (CPDs), which sever nucleic acid chains. Spirulina maxima extract repairs DNA by directly decomposing CPDs.

[0017] The combination of the above three can achieve a better anti-photodamage effect and repair the skin barrier.

[0018] In the above-mentioned composition for preventing light damage, the weight ratio of the ribonucleic acid solution, ectoine, and Spirulina maxima extract solution is 65-75:15-25:3-7.

[0019] At the same time, the present invention also discloses the use of the above composition in preparing cosmetics with anti-photodamage properties.

[0020] In addition, the present invention also discloses a cosmetic having anti-photodamage effect, which contains the composition as described above.

[0021] The above-mentioned cosmetic contains 1 to 5 wt% of the composition.

[0022] This application has at least the following beneficial effects:

[0023] The composition of the present invention uses ribonucleic acid solution, ectoine, and spirulina maxima extract solution as core components, and can achieve good anti-light damage effect. Samples were sent to a third-party testing agency for zebrafish embryo light-damaged cell apoptosis test, and the test results showed that the effect of the composition of the present invention is better than the cell apoptosis effect of ergothioneine at the same concentration. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a fluorescent photograph of the tail fin of the model control group;

[0025] Figure 2 is a fluorescent photograph of the tail fin of the positive control group;

[0026] Figure 3 is a fluorescent photograph of the tail fin of the tested sample group;

[0027] Figure 4 It is a bar graph showing the results of the anti-photodamage efficacy test.

[0028] Specific embodiment

[0029] Below in conjunction with embodiments of the present invention, the present invention is clearly and completely described, in description of the present invention, it should be noted that, in the embodiment, the unrecited specific conditions person, carry out according to the condition of normal condition or manufacturer's suggestion. Reagents therefor or instrument are not recited manufacturer person, are the conventional products that can be obtained by commercial purchase. When not making special instructions, used part in the embodiments of the present invention is all weight part.

[0030] Source of raw materials

[0031] The supplier and brand name of the RNA solution are: Angel Yeast Co., Ltd.; Yeast Essence N80 (N); the active ingredient is RNA, and its content is 3.5 wt%;

[0032] The suppliers and trade names of ectoine are: Zhejiang Huarui Biotechnology Co., Ltd., Ectoine;

[0033] The supplier and trade name of Spirulina maxima extract solution is: ALGAKTIV SL (a Greenaltech S.L Company); The active ingredient of GenoFix CPD CN (Propanediol) is Spirulina MAXIMA extract, with a content of 20wt%.

[0034] Preparation of composition

[0035] The RNA solution, ectoine and Spirulina maxima extract solution are mixed at room temperature to form a homogeneous phase.

[0036] The formula table can be found in Table 1;

[0037] Table 1 Recipe Unit: Weight ratio

[0038]

[0039] 1. Performance testing and moisturizing test

[0040] 1.1 Test method:

[0041] By recruiting 35 healthy Chinese male and female subjects with arm stratum corneum moisture content ranging from 15 to 45 (Corneometer Unit, CU), the changes in skin stratum corneum moisture content before and after product use were used to evaluate the efficacy of the sample ingredients in moisturizing cosmetics, providing a basis for the product's moisturizing efficacy claims.

[0042] 1.2 Experimental basis and source

[0043] QB / T 4256-2011 "Guidelines for the Evaluation of Moisturizing Efficacy of Cosmetics"

[0044] 1.3 Instruments and Equipment

[0045] Corneometer CM 825 skin moisture meter (Courage+Khazaka, Germany)

[0046] 1.4 Test environment requirements

[0047] The test environment temperature is 20℃-22℃, humidity is 40%-60%, and real-time monitoring is performed.

[0048] 1.5 Sample:

[0049] The above examples and comparative examples were diluted at a volume ratio of 1:50, and the diluted samples were used for

[0050] The subjects were randomly divided into seven groups, A, B, C, D, E, F, and G, with 5 subjects in each group. A left and right half-face comparison method was used, with placebo applied to the left side of the face. The subjects in group A were smeared with the diluted sample of Example 1 on their right face; the subjects in group B were smeared with the diluted sample of Example 2 on their right face; the subjects in group C were smeared with the diluted sample of Example 3 on their right face; the subjects in group D were smeared with the diluted sample of Comparative Example 1 on their right face; the subjects in group E were smeared with the diluted sample of Comparative Example 2 on their right face; the subjects in group F were smeared with the diluted sample of Comparative Example 3 on their right face; and the subjects in group G were smeared with the diluted sample of Comparative Example 4 on their right face.

[0051] Before the test, the subjects cleaned their faces uniformly for 30 minutes, and the stratum corneum water content of the apple muscle area of ​​the left and right half of the face was measured as the initial value. After that, the placebo and experimental group essences were applied, and the stratum corneum water content of the skin was measured again 4 hours after use.

[0052] Calculation formula

[0053] Relative increase rate of skin moisture content (%) = (skin moisture content of experimental group - skin moisture content of control group) / skin moisture content of control group × 100;

[0054] The test results can be found in Table 2;

[0055] Table 2 Test results

[0056]

[0057] Result analysis:

[0058] It can be seen from Examples 1 to 3 that the relative growth rate of skin moisture content in Example 2 of the present invention is the highest, indicating that the composition of the present invention has a rapid repair effect.

[0059] Comparative Examples 1, 2, and 3 show that the RNA solution has a certain effect on repairing and replenishing the skin barrier, but it needs to be used in combination with either ectoine or Spirulina maxima extract solution to show a more obvious effect. Comparative Example 4 shows from one aspect that, from the perspective of the relative growth rate of skin moisture content, the synergistic effect of ectoine and Spirulina maxima extract solution is more significant;

[0060] In terms of comprehensive performance, the effect of Example 2 is the most significant. We believe that this is the result of the synergistic relationship between the RNA solution and either the ectoine or the Spirulina maxima extract solution.

[0061] Cosmetic Safety Testing

[0062] The samples of Example 1, Example 2, and Example 3 were subjected to cosmetic safety testing;

[0063] Detection method:

[0064] Test substance: Take an appropriate amount of the sample in Example 1 and mix thoroughly at a volume ratio of 1:19 (sample: pure water) as the test substance.

[0065] Experimental animals: 4 ordinary New Zealand rabbits;

[0066] 24h before the test, the hair on both sides of the back of New Zealand rabbits was sheared off, and the hair removal area on both sides was about 3cm × 3cm, with the right side as the coating area and the left side as the blank control area. 0.5mL of Example 7 sample was measured respectively, evenly applied to the skin of the animal coating area, and applied once a day for 14 consecutive days. From the second day, hair was sheared before each application, and the residual test substance was removed with pure water after 1h of observation. The results were scored by the skin irritation / corrosion test table 1 of "Safety Technical Specifications for Cosmetics" (2015 edition). The average score of each animal per day was calculated by the following formula, and the skin irritation intensity was judged by the skin irritation / corrosion test table 2 of "Safety Technical Specifications for Cosmetics" (2015 edition).

[0067] Average score per animal per day = (∑ erythema and edema score / number of animals tested) / 14

[0068] The experimental results are shown in Table 3;

[0069] Table 3 Irritation test results

[0070]

[0071] Test conclusion

[0072] The sample of Example 1 showed multiple skin irritation to New Zealand rabbits: no irritation.

[0073] The samples of Example 2 and Example 3 were tested according to the above method; the irritation test results are shown in Tables 4 and 5;

[0074] Table 4 Irritation test results

[0075]

[0076] Table 5 Irritation test results

[0077]

[0078]

[0079] Test conclusion

[0080] The multiple skin irritation of the sample of Example 2 and the sample of Example 3 to New Zealand rabbits was: non-irritating.

[0081] Part 4: Zebrafish embryo light-damaged cell apoptosis test

[0082] The test was conducted by a third-party testing company, Vitargent (International) Biotechnology Co., Ltd., and the test report number is VI-R20241857S-4A;

[0083] Test item: Anti-light damage efficacy: zebrafish embryo light damage cell apoptosis test;

[0084] Testing Method: A 0.3J / cm² UVB irradiation model was used. Thirty three-day-old zebrafish embryos were exposed to a 5g / L solution of a DNA damage repair composition. A model control group and a blank control group were also set up. After 1 hour of exposure, the embryos were irradiated with 0.3J / cm² UVB to establish a model. The solution was then replaced. After 24 hours of incubation, the embryos were stained with acridine orange solution for 15 minutes. After the dye was washed off, the embryos were photographed under a microscope to measure the fluorescent area of ​​the tail fin and perform statistical analysis.

[0085] Test results:

[0086] Model control group: The effect of protecting against light damage was not significant (0% cell apoptosis inhibition rate). For related pictures, please refer to Figure 1 ;

[0087] Positive control group: ergothioneine (5%), which has a significant effect in preventing light damage (81% cell apoptosis inhibition rate). Related pictures can be found in the reference Figure 2 ;

[0088] Test sample group: VI-20241857S-4 DNA damage repair composition (5%), with significant light damage protection effect (86% cell apoptosis inhibition rate), related pictures can be referenced Figure 3 ;

[0089] The bar chart of the anti-photodamage efficacy test results can be referenced Figure 4 ;

[0090] Conclusion: The sample can significantly reduce the apoptosis of zebrafish embryos induced by UVB irradiation and has the effect of protecting against light damage.

[0091] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and it is intended that equivalents falling within the claims be included. All variations within the meaning and scope of the elements are encompassed by the present invention. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

Claims

1. A composition having resistance to light damage, characterized in that The invention comprises a ribonucleic acid solution, ectoine, and a spirulina maxima extract solution, wherein the weight ratio of the ribonucleic acid solution, ectoine, and spirulina maxima extract solution is 60-80:10-30:1-10; The concentration of ribonucleic acid in the ribonucleic acid solution is 3.5 wt%; The concentration of the Spirulina maxima extract in the Spirulina maxima extract solution is 20 wt %.

2. The composition for preventing light damage according to claim 1, wherein The weight ratio of the ribonucleic acid solution, ectoine, and spirulina maxima extract solution is 65-75:15-25:3-7.

3. Use of the composition according to claim 1 or 2 in preparing cosmetics having anti-photodamage properties.

4. A cosmetic with anti-photodamage properties, characterized in that: It contains the composition according to claim 1 or 2.

5. The cosmetic according to claim 4, characterized in that The composition contains 1 to 5 wt%.

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