9 results about "Bioluminescence imaging" patented technology

The invention relates to the technical field of bacterium detection. The invention provides a bacteriophage labeled array micro-fluidic chip based on dot matrix luminescence quantitative analysis, a preparation method thereof and a method for detecting by using the same, target pathogenic bacteria are enriched by adopting magnetic beads, and specific recognition and capture of virulent strains are realized by combining an array micro-fluidic chip integrated with a bacteriophage coding probe. And after the captured viable bacteria are cracked to release ATP, bioluminescence imaging and quantitative detection are realized. The method comprises the following steps: enriching and eluting to-be-detected bacteria through magnetic beads modified with bacteriophage, then introducing a sample into an array micro-fluidic chip system, and realizing rapid qualitation and accurate quantification of live strains according to the distribution area of light-emitting signals and the number of light-emitting points in an array. The chip detection system has the characteristics of high efficiency and rapidness, can finish on-site instant detection of at most 12 food-borne pathogenic viable bacteria and virulent strains thereof within 30 minutes, and provides a new method for food safety monitoring.

The invention discloses a micro / nano plastic based on organic long-afterglow material labeling and a preparation method and application thereof, and belongs to the technical field of crossing of environmental science and macromolecular nano materials. According to the method, organic long-afterglow material dispersion liquid is dropwise added into micro / nano plastic dispersion liquid, and swelling is balanced; and carrying out rotary evaporation to remove the swelling agent, washing, and dialyzing until the dialysate has no obvious ultraviolet absorption, so as to obtain the micro / nano plastic marked by the organic long afterglow material. According to the method, the characteristic that the organic long-afterglow material can realize high-brightness luminescence without in-situ excitation is utilized, and the background interference of an environmental medium is effectively inhibited, so that the signal-to-noise ratio and the sensitivity of micro / nano plastic detection are remarkably improved under the environmental concentration condition. Compared with an existing marking technology, the method has the advantages that the operation is simple, the cost is low, the biocompatibility is good, and the problems of false positive interference and photobleaching can be effectively avoided; good application prospects are shown in the fields of environment migration behavior research of micro / nano plastic, bioluminescence imaging and the like.

The invention provides an adjustable bioluminescence imaging lighting device based on a fiber Bragg grating. The adjustable bioluminescence imaging lighting device comprises a light source; the first lens unit is used for converting light rays emitted by the light source into parallel light rays; the rotary light splitting unit is used for enabling all the parallel light rays to penetrate or splitting the parallel light rays into transmission light and reflected light; the second lens unit is used for transmitting the focused light and inputting the focused light to the first fiber bragg grating through the first coupler; the first fiber bragg grating is used for reflecting the narrow-band light of the first preset wave band and outputting the narrow-band light through the output end of the first coupler; the third lens unit is used for focusing the reflected light and inputting the focused reflected light to a second fiber bragg grating through a second coupler; the second fiber bragg grating is used for reflecting narrow-band light of a second preset wave band and outputting the narrow-band light through the output end of the second coupler; and the third coupler is used for mixing the input light rays of the first / second preset wave bands and then outputting the mixed light rays to the light source input end of the endoscope. The device can be used for clinical diagnosis and detection.

This invention belongs to the field of tumor biology and drug screening technology, specifically disclosing a human EGFR mutation-driven mouse primary lung cancer cell line, its construction method, and its applications. The cell line, ZST-1, is a human EGFR (L858R / T790M) mutation-driven lung cancer cell line derived from mouse primary lung cancer. It is stable, capable of subcutaneous tumor formation in C57BL / 6 mice, and simultaneously expresses Luciferase and tdTomato reporter genes. Its construction method includes obtaining transgenic mice, virus-induced tumor formation, continuous in vivo passage in nude mice, and in vitro culture and screening steps. This cell line can be applied to in vitro screening and efficacy evaluation of human EGFR mutation-targeting drugs, research on EGFR-TKI resistance mechanisms, tumor bioluminescence imaging and fluorescence tracing, and in vivo tumorigenesis and efficacy experiments in an immune-intact C57BL / 6 background.

The invention belongs to the technical field of biological living body imaging, and provides a multi-modal fusion three-dimensional imaging device, which comprises a first box body provided with a main camera shooting unit, an auxiliary camera shooting unit, a 3D camera shooting unit and a first light source; the second box body is arranged in the first box body; a movable first top plate is arranged on the base; the objective table is arranged in the second box body, the first top plate is located between the main camera shooting unit and the objective table, the objective table and the 3D camera shooting unit can rotate relatively, the 3D camera shooting unit and the auxiliary camera shooting unit are obliquely arranged relative to the objective table, and the first light source is used for providing a needed light source for bioluminescence imaging; the main camera shooting unit and the auxiliary camera shooting unit are used for shooting multi-angle two-dimensional bioluminescence images, the 3D camera shooting unit is used for scanning and shooting three-dimensional model contour data, the X-ray mechanism is used for shooting two-dimensional X-ray skeleton images of a measured living body, and registration is carried out through images of multiple modes, so that an accurate three-dimensional model of the measured living body is obtained.

The present invention provides a bioluminescence imaging flow cytometry method and apparatus, comprising: a free reflection unit configured to obtain a bioluminescence signal by irradiating a sample suspension, and to re-reflect and converge the collected bioluminescence signal to enhance the detected bioluminescence signal; the sample control unit is configured to comprise a multi-channel flow type assembly, a fixing mechanism and a three-axis displacement table; the multi-channel flow type assembly is used for fixing and bearing a sample suspension, the fixing mechanism is used for fixing the multi-channel flow type assembly, and the three-axis displacement table drives the fixing mechanism to perform three-axis movement so as to adjust an imaging area of the sample suspension fixedly borne by the multi-channel flow type assembly; and an optical imaging unit configured to capture a bioluminescent cell image at a low signal level in space.

This invention proposes a microneedle patch that seamlessly integrates infection-activated bioluminescence imaging, photodynamic therapy, and synergistic antibacterial activity with carbon monoxide gas to inhibit bacterial infection in wounds using anthocyanin-derived pH-responsive photofunctional molecules. The invention presents a high-performance phototherapy agent based on anthocyanin derivatives that can sense and dynamically adjust its fluorescence and photodynamic therapy (PDT) properties in response to changes in acidity within the infection microenvironment. Subsequently, a hydrophobic probe is co-assembled with a reactive oxygen species (ROS) CO donor to form a biocompatible therapeutic nanosystem, which is further encapsulated in a hyaluronic acid microneedle patch to enhance its mechanical penetration and controlled-release activity at the infection site. The binding of PDT with the CO gas cascade produces a powerful bacterial elimination effect, significantly inhibiting bacterial biofilm-mediated infection. This ingenious combination of infection-activated bioimaging and synergistic antibacterial therapy offers significant potential for the effective and personalized management of infection-related diseases.

This invention provides a near-infrared bioluminescent probe of thionitrite, its preparation method, and its application. The bioluminescent probe BF-5 has good selectivity, stability, biocompatibility, and a low detection limit (327 nM). It can detect the physiological level of HSNO in HCT116-luc cells and can be applied to bioluminescent imaging of HSNO in HCT116-luc colon cancer xenografts in nude mice. It provides a visual detection tool for revealing the physiological and pathological mechanisms of HSNO and is of great significance for studying diseases related to HSNO.

The present application relates to the technical field of bacterial detection. The present application provides a phage-labeled array microfluidic chip based on quantitative analysis of dot array luminescence, a preparation method thereof and a detection method using the same. The target pathogenic bacteria are enriched by magnetic beads, and the array microfluidic chip integrated with phage coding probes is combined to realize specific recognition and capture of strong strains. After the captured live bacteria are lysed to release ATP, bioluminescence imaging and quantitative detection are realized. The process is as follows: the bacteria to be tested are enriched and eluted by magnetic beads modified with phages, then the sample is introduced into the array microfluidic chip system, and according to the distribution area and the number of luminescent points in the array, rapid qualitative and accurate quantitative detection of live strains can be realized. The chip detection system has the characteristics of high efficiency and rapidness, and can complete on-site real-time detection of up to 12 kinds of foodborne pathogenic live bacteria and their strong strains within 30 minutes, thereby providing a new method for food safety monitoring.