2 results about "Bioluminescence imaging" patented technology

This invention belongs to the field of tumor biology and drug screening technology, specifically disclosing a human EGFR mutation-driven mouse primary lung cancer cell line, its construction method, and its applications. The cell line, ZST-1, is a human EGFR (L858R / T790M) mutation-driven lung cancer cell line derived from mouse primary lung cancer. It is stable, capable of subcutaneous tumor formation in C57BL / 6 mice, and simultaneously expresses Luciferase and tdTomato reporter genes. Its construction method includes obtaining transgenic mice, virus-induced tumor formation, continuous in vivo passage in nude mice, and in vitro culture and screening steps. This cell line can be applied to in vitro screening and efficacy evaluation of human EGFR mutation-targeting drugs, research on EGFR-TKI resistance mechanisms, tumor bioluminescence imaging and fluorescence tracing, and in vivo tumorigenesis and efficacy experiments in an immune-intact C57BL / 6 background.

The present application relates to the technical field of bacterial detection. The present application provides a phage-labeled array microfluidic chip based on quantitative analysis of dot array luminescence, a preparation method thereof and a detection method using the same. The target pathogenic bacteria are enriched by magnetic beads, and the array microfluidic chip integrated with phage coding probes is combined to realize specific recognition and capture of strong strains. After the captured live bacteria are lysed to release ATP, bioluminescence imaging and quantitative detection are realized. The process is as follows: the bacteria to be tested are enriched and eluted by magnetic beads modified with phages, then the sample is introduced into the array microfluidic chip system, and according to the distribution area and the number of luminescent points in the array, rapid qualitative and accurate quantitative detection of live strains can be realized. The chip detection system has the characteristics of high efficiency and rapidness, and can complete on-site real-time detection of up to 12 kinds of foodborne pathogenic live bacteria and their strong strains within 30 minutes, thereby providing a new method for food safety monitoring.