Xanthene-hemicyanine near-infrared fluorescent dye as well as synthesis method and application thereof

Abstract

The invention discloses a xanthene-hemicyanine near-infrared fluorescent dye as well as a synthesis method and application thereof. The dye has longer absorption and emission wavelengths, the water solubility of the dye is improved, and the change of spectral properties after the dye interacts with biomacromolecules is avoided by reasonably introducing active groups. Experiments prove that the dye has good photophysical properties and bioluminescence imaging potential, and can be applied to the aspects of biological recognition imaging, cell imaging, protein labeling, specific recognition of antibodies, nucleic acid labeling, DNA sequencing, tumor photodynamic therapy and the like.

Description

technical field [0001] The invention belongs to the technical field of organic dyes, and relates to a class of xanthene-hemicyanine near-infrared fluorescent dyes, a synthesis method thereof, and applications thereof. Background technique [0002] For a long time, fluorescent dyes have been widely used in the field of biomarkers and identification, and play an important role in the sequencing of biologically specific proteins, tumor markers and DNA recognition genes. [0003] Compared with the isotope labeling method, the fluorescence analysis method has the advantages of simple equipment, convenient operation, high sensitivity and fast response. The fluorescent dyes used in fluorescence analysis play a pivotal role in the entire analysis process, and the performance of the fluorescent dyes will directly affect the accuracy and sensitivity of the analysis. At present, the common commercial fluorescent dyes are mainly coumarin, fluorescein, rhodamine, indocyanine green and s...

AI Technical Summary

Problems solved by technology

However, these dyes have defects to some extent that affect their application

For example: 1. The absorption wavelength and emission wavelength are relatively short, and it is easy to receive interference from biological background fluorescence during imaging and analysis; 2. The water solubility is generally poor, and it is easy to aggregate in aqueous solvents to cause fluorescence quenching; 3. The photostability is poor, which increases the cost of large-scale storage and transportation of products, and makes it impossible to repeat the detection; 4. After these dyes are combined with biomacromolecules, they may be affected by biomacromolecules, resulting in changes in their own photophysical properties, thus Affect the results of detection and analysis; 5. The molar extinction coefficient and fluorescence quantum yield at a specific wavelength are low, which cannot be well matched with commonly used light sources and detectors on the market

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