Method of controlling the quality of salvia miltiorrhiza raw material fingerprint in the plant medicine for improving hemorheology

A quality control method and fingerprint technology, applied in the field of quality control of salvia miltiorrhiza raw materials, can solve few problems such as quality control of traditional Chinese medicinal materials

Active Publication Date: 2007-09-26
ANHUI ZHIHETANG PHARMACY
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Problems solved by technology

[0010] There are some research reports on the fingerprints of Danshen medicinal materials, but t...

Method used

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  • Method of controlling the quality of salvia miltiorrhiza raw material fingerprint in the plant medicine for improving hemorheology
  • Method of controlling the quality of salvia miltiorrhiza raw material fingerprint in the plant medicine for improving hemorheology
  • Method of controlling the quality of salvia miltiorrhiza raw material fingerprint in the plant medicine for improving hemorheology

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Embodiment 1

[0048] Accurately weigh about 1.0 g of Salvia miltiorrhiza medicinal material powder (passed through a No. 3 sieve), add 100 ml of 50% methanol, accurately weigh, extract with microwave for 70 minutes, cool, and weigh for additional weight. Shake well, filter with a 0.45 μm microporous membrane, and take the subsequent filtrate for HPLC fingerprint analysis.

[0049] Chromatographic column: Alltima C18 column (4.6mm×250mm, 5μm) of Alltech Company, column temperature 20°C, detection wavelength 280nm, flow rate 1.0ml / min, injection volume 20μl. The samples were eluted using a gradient, and the mobile phase and gradient settings are shown in Table 1.

[0050] time (min)

A(0.4% formic acid) / %

B(acetonitrile) / %

0

40

50

70

90

63

18

15

10

37

82

85

[0051] By calculating the similarity of ten batches of Danshen medicinal materials, it was found that the similarity of medicinal material...

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Abstract

The invention relates to a DanShen fingerprint spectrum quality control method, comprising that (1), adding 1.0g DanShen powder into carbinol to extract via microwave, (2), washing flow phase gradient that the Alltima C18 column is 4.6mm, 250mm, and 5mum, checking wavelength is 280nm, flow speed is 1.0ml/min, the column temperature is 20-40Deg. C, and the sample amount is 10-20ul, (3), building standard fingerprint spectrum that the first peak is DanShen element, the eighth peak is rosmarinic acid, the ninth peak is alkannic acid, the tenth peak is phenolic acid B, the eleventh peak is phenolic acid B isomer, the fourteenth peak is cryptotanshinone and the fourteenth peak is tanshinone IIA, (4), controlling the quality of fingerprint spectrum that the check peak relative holding times are 0.21 of DanShen element, 0.91 of rosmarinic acid, 0.93 of alkannic acid, 1.00 of phenolic acid B, 1.04 of phenolic acid B isomer, 0.92 of cryptotanshinone and 1.00 of tanshinone IIA, (5), the DanShen planting collecting method. The invention can control the quality of materials to assure the stable quality of product.

Description

technical field [0001] The invention belongs to the field of quality control methods for salvia miltiorrhiza raw materials, in particular to a method for quality control of fingerprints of salvia miltiorrhiza raw materials in herbal medicines for strengthening health and removing blood stasis. Background technique [0002] Salvia (Radix et Rhizoma Salviae Miltiorrhizae) is the dry root and rhizome of Salvia miltiorrhiza Bunge, a plant of Labiatae. It is named "Danshen" because of its red color and ginseng-like shape. Alias: purple salvia, red salvia, blood ginseng, red ginseng, red root, Sichuan salvia, wild salvia, etc. Its taste is bitter, slightly cold in nature, Guixin and Liver Meridian. As a traditional Chinese medicine, it has been used for a long time in our country. It was first recorded in "Shen Nong's Materia Medica", and it was listed as the top grade. "Compendium of Materia Medica", "Compendium of Materia Medica", "Women Ming Theory", "Southern Yunnan Materia...

Claims

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Application Information

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IPC IPC(8): A61K36/537A61P9/10A61P15/00G01N30/02G01N33/86A01G1/00A61K31/125
Inventor 胡坪潘一峰
Owner ANHUI ZHIHETANG PHARMACY
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