Method for preparing low molecular weight brown seaweed fucoidan sulfate

A brown algae fucoidan sulfate, low molecular weight technology, used in blood diseases, extracellular fluid diseases, antitumor drugs, etc. , reaction conditions and difficult control of products, etc., to achieve the effect of fast separation, simple and fast desalination steps, and complete automatic consumption

Inactive Publication Date: 2011-02-02
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the molecular weight distribution of oligosaccharides obtained by acid hydrolysis of fucoidan sulfate is difficult to control, the yield is relatively low, and the composition of the obtained polysaccharides is uneven, the desulfurization phenomenon is relatively serious, and the desalination step is troublesome
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  • Method for preparing low molecular weight brown seaweed fucoidan sulfate
  • Method for preparing low molecular weight brown seaweed fucoidan sulfate

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Embodiment 1

[0014] The brown algal sulfated polysaccharide raw sugar is prepared from brown algae such as kelp, hijiki, wakame, sargassum, ascophyllum nodosum or fucus, and the preparation method is according to the technology disclosed in the invention patent 98110253.0. Commercially available brown alginate sulfated polysaccharide can also be used.

[0015] Preparation of low molecular weight fucoidan sulfate:

[0016] 1) Oxidative degradation of brown algal sulfated polysaccharides by hydroxyl radicals: add 2.0g of brown algal sulfated polysaccharides to 20mL of water to dissolve to make an aqueous solution of brown algal sulfated polysaccharides with a concentration of 100mg / mL; then add 0.16g of copper acetate monohydrate to the copper ions in the raw sugar solution The concentration is 0.04mol / L; after adjusting the pH of the solution to 7.5 with NaOH, heat it in a water bath to 60°C and keep the temperature constant; Stir while adding, stop adding hydrogen peroxide solution after ...

Embodiment 2

[0022] 1) Hydroxyl radical oxidative degradation of brown algal sulfated polysaccharide: add 6.75g of kelp sulfated polysaccharide rough sugar to 45mL of water and dissolve to make an aqueous solution of brown algal sulfated polysaccharide with a concentration of 150mg / mL; then add 0.36g of copper acetate monohydrate to the raw sugar The copper ion concentration in the aqueous solution is 0.04mol / L; after adjusting the pH of the solution to 7.5 with NaOH, heat it in a water bath to 60°C and keep the temperature constant; then add 4.5% hydrogen peroxide solution at a rate of 0.9mL / min, and add Stir, and stop adding hydrogen peroxide solution after reacting for 5 hours; add 2mol / L NaOH to the reaction solution to adjust the pH to 8, then centrifuge to remove the precipitate; take the centrifuged supernatant, adjust the pH to neutral with hydrochloric acid, Flow the supernatant into the Chelex 100 chelation column at a speed of 10mL / min, collect the eluate to obtain the degradatio...

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Abstract

The invention relates to a method for preparing low molecular weight brown seaweed fucoidan sulfate. The method comprises a step of oxidatively degrading brown seaweed sulfated polysaccharides by hydroxyl radical and a step of ultrafiltration membrane separation and purification, wherein raw sugar of the brown seaweed sulfated polysaccharide is used as a raw material, the hydroxyl radical generated by the reaction of copper ions and hydrogen peroxide is adopted to oxidatively degrade the raw sugar of the brown seaweed sulfated polysaccharide, and the membrane separation technology is combined in the method for preparing the low molecular weight fucoidan sulfate from the brown seaweeds. In the preparation process, the oxidizing speed is uniform, the reacting condition is mild, and the controllability and repeatability are good; the copper ions are removed by a chelating column after the oxidation reaction is finished, so the operation process is simple. Furthermore, the pH value is adjusted by adding little acid and alkali, so the salt content in the product is low, and can be completely removed by a 300Da ultrafiltration membrane in the ultrafiltration membrane separation and purification process. The ultrafiltration membrane can effectively separate and purify the brown seaweed fucoidan with different molecular weights along with high separating speed and large treatment amount. The method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of polysaccharide preparation, and in particular relates to a preparation method of low molecular weight fucoidan sulfate ester. Background technique [0002] Fucoidan sulfate is a unique sulfated polysaccharide in the cell wall of brown algae. The crude fucoidan sulfate extracted from brown algae by acid extraction and hot water extraction is a heteropolysaccharide with a molecular weight of about 100kDa to 800kDa. The main constituent unit is 1,2-α-fucose, with a sulfate group on C4, and xylose, mannose, galactose, glucose and rhamnose on the sugar chain, and a small number of arabinose; its uronic acid Mainly glucuronic acid or mannuronic acid, some contain galacturonic acid. The results of the study found that fucoidan sulfate in brown algae such as Fucus, Fucus, Staghorn, Ascophyllum nodosum, and Kelp has significant physiological effects such as anti-thrombotic, anti-tumor, and anti-virus, and has anti-inflamma...

Claims

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Application Information

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IPC IPC(8): A61P35/00C08B37/00A61P31/12A61P7/02
Inventor 赵雪李八方董诗竹李芳
Owner OCEAN UNIV OF CHINA
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