Method for extracting brown algae polysaccharide sulfated

A technology of fucoidan and sulfate ester, which is applied in the field of marine chemical engineering, can solve problems affecting product yield, production cost, and waste of resources, and achieve the effect of saving resources and simple operation

Inactive Publication Date: 2007-09-19
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existence of a large number of "sugar gum" not only brings great trouble and difficulty to the extraction of iodine and mannitol, but also seriously aff

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  • Method for extracting brown algae polysaccharide sulfated

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Embodiment 1

[0014] Add 1ml of chitosan acetic acid solution with an acid solution concentration of 1% and a molecular weight of 2000 to 150ml of kelp soaking liquid, stir for 2 minutes to flocculate for 4 hours, and then centrifuge at a speed of 500 rpm for 2 minutes to separate and obtain a precipitate Swell for 4 hours under the acidic condition of pH 2 (adjust the pH with hydrochloric acid), and stir for 2 hours to dissolve, filter, adjust the filtrate to neutral (use 10% NaOH solution to adjust to neutral), concentrate under reduced pressure, and concentrate The liquid reaches 1 / 10 of the original solution, and then the ethanol concentration is added to the concentrated solution to reach 65%, and the precipitate is filtered, washed twice with anhydrous ether, and freeze-dried with the FD-5N freeze dryer of Tokyo Physical and Chemical Company, namely Fucoidan sulfate was obtained (see Figure 1), and the obtained fucoidan accounted for 10.51% of the precipitated amount.

Embodiment 2

[0016] Add 2ml of chitosan acetic acid solution with an acid solution of 2% and a molecular weight of 760,000 to 150ml of kelp soaking liquid, stir for 4 minutes to flocculate for 0.5 hours, and then centrifuge at a speed of 3000 rpm for 3 minutes to separate and obtain a precipitate Swell for 1 hour under an acidic condition with a pH of 4, stir for 2 hours to dissolve, filter, adjust the filtrate to neutrality, concentrate under reduced pressure, then add ethanol to the concentrated solution to make the concentration reach 70%, filter to obtain a precipitate, and use the free The fucoidan was washed twice with water and ether, and freeze-dried to obtain the fucoidan sulfate, which accounted for 5% of the precipitated amount.

Embodiment 3

[0018] Add 8ml of chitosan hydrochloric acid solution with a molecular weight of 3% and 80,000 to 150ml of kelp soaking liquid, stir for 8 minutes to make it flocculate for 8 hours, and then centrifuge at a speed of 1500 rpm for 5 minutes to separate and obtain a precipitate Swell for 24 hours under an acidic condition with a pH of 6, stir for 4 hours to dissolve, filter, adjust the filtrate to neutrality, concentrate under reduced pressure, then add ethanol to the concentrated solution to make the concentration reach 75%, filter to obtain a precipitate, and use the free Wash twice with water and acetone, and dry in vacuum to obtain fucoidan sulfate, which accounts for 11.1% of the precipitated amount.

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Abstract

The invention relates to ocean chemical engineering technique field, concretely is a method for extracting fucoidan from a kelp soak solution, which includes steps: adding 1-10 ml flocculant to kelp soak solution per 150 ml, stirring for 2-10 min, flocculating for 10 min to 8 h, swelling for 1-24 h under condition that pH is 2-6 after centrifuging and depositing, stirring for 1-4 h to dissolve, filtrating and adjusting the filtrate to neutrality, decompressing and concentrating, adding ethanol to the concentrated solution, filtrating to obtain deposition, washing and drying the deposition to obtain the fucoidan. The invention relates to employs chitosan and its derivative as flocculant to flocculate and deposit ''sugar glue'' from kelp soak solution, and then extracts fucoidan from the ''sugar glue''. The advantages of the invention are: resources are utilized comprehensively, operation is simply, quantity of used acid and base is reduced, and quantity of the obtained fucoidan reaches 5-16% of quantity of the deposition.

Description

technical field [0001] The invention relates to the technical field of marine chemical engineering, in particular to a method for extracting fucoidan sulfate from kelp soaking liquid. Background technique [0002] Fucoidan sulfate or sulfated polysaccharide is an intercellular polysaccharide present in all brown algae, also known as fucoidan and fucoidan. In 1913, it was first extracted from the brown seaweed Laminaria palmatum by Kylin. The composition of fucoidan sulfate is very complex, and it varies greatly in different brown algae species. Recent studies have shown that it has anticoagulant, blood circulation, blood lipid regulation, anti-tumor, anti-virus, enhanced cellular immunity and humoral immunity, and anti-inflammatory effects. Aging, protection of basal cells and other biological activities. [0003] At the end of the 1950s, due to the development of the artificial cultivation of kelp, the output of kelp increased sharply. In addition to being used for food, ...

Claims

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Application Information

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IPC IPC(8): C08B37/04
Inventor 李鹏程陈晓琳邢荣娥于华华刘松
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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