Method for extracting fucoidan for brown seaweed

Abstract

The invention relates to a fucoidan extract method, in particular to a method for extracting fucoidan for brown seaweed, comprising the following steps: 1. desalting: adopting fresh brown seaweed or unfreezed frozen brown seaweed to soak in pure water to remove salt; 2. enzymolysis: adding enzyme in the desalted brown seaweed to perform enzymolysis at 40-60 DEG C for 1-3h; 3. abstraction: adding calcium chloride in enzymatic hydrolyzate obtained by enzymolysis, and heating the solution to 90-100 DEG C for 1-4h to obtain the fucoidan extract. The invention is characterized of simple operation, high application security, high extraction efficiency, high extraction purity and the like and the extraction process is applicable to factory mass production.

Description

technical field

[0001] The invention relates to a method for extracting fucoidan sulfate. Background technique

[0002] Fucoidan sulfate (fucoidan), also known as fucoidan sulfate, also known as fucoidan or fucoidan. Fucoidan sulfate is mainly found in brown algae and is an intercellular polysaccharide inherent in all brown algae, present in the cell wall matrix. Generally, in brown algae that grow in the intertidal zone, such as perennial fucus, the content of fucoidan sulfate can be as high as 20%, and the content in kelp that grows in deeper depths is lower, about 1 to 2%. . In 1913, Kylin first proposed fucoidan sulfate from the brown seaweed Laminaria palmatum. Fucoidan sulfate has a complex structure, has a variety of physiological activities, and has obvious anticoagulant and fibrinolytic pharmacological activities; fucose containing sulfate groups or its degradation products can induce apoptosis of cancer cells and can be used As an apoptosis inducer and anticanc...