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Method for extracting fucoidan for brown seaweed

A technology of fucoidan sulfate and brown algae, which is applied in the field of extraction of fucoidan sulfate, can solve the problems of unfavorable factory production, low extraction purity, complicated production process, etc., and achieves no toxic side effects and high extraction purity High, high application security effect

Active Publication Date: 2010-01-06
大连海宝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method of extracting fucoidan sulfate uses many kinds of reagents in the intermediate treatment stage, and some of them, such as formaldehyde, are harmful to the human body and increase potential safety hazards; the extraction purity is not high, and the fucoidan sulfate obtained by some extraction methods The content in the final extract is less than 20%; the production process is complicated, and the treatment process is respectively subjected to methods such as ultrasound, decompression concentration or electrodialysis, which consumes a lot of energy and costs, which is not conducive to factory production; the production steps must be repeated precipitation , pH adjustment, centrifugal precipitation and other operations are very cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The first step is to remove salt: take fresh or frozen wakame spore leaves, after thawing at room temperature, take 100 kg and add 200 kg of pure water, soak for 15 minutes, remove water, repeat soaking and removing water once, remove salt;

[0023] The second step of enzymatic hydrolysis: add 200 kg of pure water to the desalted wakame spore leaves, add 0.5 kg of cellulase for enzymatic hydrolysis, the enzymatic hydrolysis temperature is 40 ° C, and the enzymatic hydrolysis time is 3 hours;

[0024] The third step of extraction: add 0.5 kg of calcium chloride to the enzymolysis solution after enzymolysis, heat to 95°C, and extract for 3.5 hours to obtain an extract solution, which is concentrated and dried to obtain a dry powder of the crude extract. The content of fucoidan sulfate in the prepared dry powder is 42%.

Embodiment 2

[0026] The first step is to remove salt: take fresh or frozen wakame spore leaves, after thawing at room temperature, take 100 kg and add 200 kg of pure water, soak for 25 minutes, remove water, repeat soaking and removing water once, remove salt;

[0027] The second step of enzymatic hydrolysis: add 350 kg of pure water to the desalted wakame spore leaves, add 2 kg of cellulase for enzymatic hydrolysis, the enzymatic hydrolysis temperature is 40°C, and the enzymatic hydrolysis time is 2.5 hours;

[0028] The third step of extraction: adding 0.5 kg of calcium chloride to the enzymolysis solution after enzymolysis, heating to 100°C, and extracting the extract for 2.5 hours. The extract was concentrated and dried to obtain dry powder of crude extract. The content of fucoidan sulfate in the prepared dry powder is 46%.

Embodiment 3

[0030] The first step is to remove salt: take fresh or frozen wakame spore leaves, after thawing at room temperature, take 100 kg and add 400 kg of pure water, soak for 45 minutes, remove water, repeat soaking and removing water once, remove salt;

[0031] The second step of enzymatic hydrolysis: add 600 kg of pure water to the desalted wakame spore leaves, add 2 kg of cellulase for enzymolysis, the enzymolysis temperature is 45°C, and the enzymolysis time is 2 hours;

[0032] The third step of extraction: adding 1 kg of calcium chloride to the enzymolysis solution after enzymolysis, heating to 96° C., extracting the extract for 4 hours, and concentrating and drying the extract to obtain dry powder of crude extract. The content of fucoidan sulfate in the prepared dry powder is 55%.

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PUM

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Abstract

The invention relates to a fucoidan extract method, in particular to a method for extracting fucoidan for brown seaweed, comprising the following steps: 1. desalting: adopting fresh brown seaweed or unfreezed frozen brown seaweed to soak in pure water to remove salt; 2. enzymolysis: adding enzyme in the desalted brown seaweed to perform enzymolysis at 40-60 DEG C for 1-3h; 3. abstraction: adding calcium chloride in enzymatic hydrolyzate obtained by enzymolysis, and heating the solution to 90-100 DEG C for 1-4h to obtain the fucoidan extract. The invention is characterized of simple operation, high application security, high extraction efficiency, high extraction purity and the like and the extraction process is applicable to factory mass production.

Description

technical field [0001] The invention relates to a method for extracting fucoidan sulfate. Background technique [0002] Fucoidan sulfate (fucoidan), also known as fucoidan sulfate, also known as fucoidan or fucoidan. Fucoidan sulfate is mainly found in brown algae and is an intercellular polysaccharide inherent in all brown algae, present in the cell wall matrix. Generally, in brown algae that grow in the intertidal zone, such as perennial fucus, the content of fucoidan sulfate can be as high as 20%, and the content in kelp that grows in deeper depths is lower, about 1 to 2%. . In 1913, Kylin first proposed fucoidan sulfate from the brown seaweed Laminaria palmatum. Fucoidan sulfate has a complex structure, has a variety of physiological activities, and has obvious anticoagulant and fibrinolytic pharmacological activities; fucose containing sulfate groups or its degradation products can induce apoptosis of cancer cells and can be used As an apoptosis inducer and anticanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 王奇隋国斌许淑芬胡绍民潘雪琴
Owner 大连海宝生物技术有限公司
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