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37results about How to "Use less enzyme" patented technology

Preparation method of 1,3-dioleoyl-2-palmitoyl triglyceride

The invention discloses a preparation method of 1,3-dioleoyl-2-palmitoyl triglyceride, which comprises the following steps: carrying out esterification reaction on glycerol and palmitinic acid under the action of a catalyst to obtain tripalmitin; carrying out ester exchange reaction on the tripalmitin and oleic acid or oleate under the action of 1,3-specific lipase until the ester exchange rate reaches 30-60%, and removing free fatty acids or fatty acid esters in the reaction system; and adding oleic acid or oleate into the reaction system, continuing the reaction until the ester exchange rate reaches 60-90%, and separating and purifying the 1,3-dioleoyl-2-palmitoyl triglyceride from the reaction products. The method disclosed by the invention has the advantages of wide raw material sources, high economy, high safety, mild technological conditions, short reaction time, low energy consumption and low enzyme consumption; and the method can be used for preparing high-yield high-purity 1,3-dioleoyl-2-palmitoyl triglyceride, and can be used as a food nutrient reinforcer for blending infant formula milk powder.
Owner:ZANYU TECH GRP CO LTD

Zymolysis rice milk beverage and preparation method thereof

The invention discloses a fermentation rice milk drink and a preparation method thereof, which adopts fermentation rice as raw material that is processed through immersion, water bathing, cooking, fermentation, beating and pulp refining, and then the pH value of the obtained emulsion is adjusted; thermostable alpha-amylase Unikamyl HT is added according to a portion of 10 kilograms of the material being added with 3 to 5ml of thermostable alpha-amylase Unikamyl HT; then the obtained starch milk is sent into the ejector device through a flow meter; through flash cooking, the temperature of the obtained material should rise to 95 to 98 DEG C; then thermal insulation, enzyme stabilization, cooling and pH value regulation are done; efficient glucoamylase Unikase GA is added according to the portion of 10 kilograms of the material being added with 5 to 15ml of efficient glucoamylase Unikase GA; the temperature of the obtained material should rise to 100 DEG C, and the killing of enzyme is done; next, the obtained material are added with water, citric acid, xanthan and sodium carboxymethyl cellulose; the temperature raises by 55 to 60 DEG C so as to do secondary homogeneous; finally, the super-high-temperature sterilization and aseptic packing are done. The fermentation rice has specific flavor of rice wine, and is characterized by sweet and fragrant taste, light yellow or ivory color, being rich in nutrition and with the functions of health care and skin-maintaining and stable storage.
Owner:SOUTH CHINA UNIV OF TECH

Process for preparing and refining galactomannan-oligosaccharide by microwave combined enzyme method

The invention discloses a process for preparing and refining galactomannan-oligosaccharide by a microwave combined enzyme method, which prepares the galactomannan-oligosaccharide by taking galactomannan glue endosperm slices as raw materials through the steps of kneading alkalization, microwave treatment, enzymolysis, membrane separation refining, and spray drying. The process has the advantages of wide raw material source, short production period, low cost, high oligosaccharide content and the like, and the product can be used as a functional food raw material.
Owner:江苏博立生物制品有限公司

Method for extracting fucoidan for brown seaweed

The invention relates to a fucoidan extract method, in particular to a method for extracting fucoidan for brown seaweed, comprising the following steps: 1. desalting: adopting fresh brown seaweed or unfreezed frozen brown seaweed to soak in pure water to remove salt; 2. enzymolysis: adding enzyme in the desalted brown seaweed to perform enzymolysis at 40-60 DEG C for 1-3h; 3. abstraction: adding calcium chloride in enzymatic hydrolyzate obtained by enzymolysis, and heating the solution to 90-100 DEG C for 1-4h to obtain the fucoidan extract. The invention is characterized of simple operation, high application security, high extraction efficiency, high extraction purity and the like and the extraction process is applicable to factory mass production.
Owner:大连海宝生物技术有限公司

Method for extracting tea oil by demulsification based on aqueous enzymatic method

InactiveCN104928012AFully formedIncrease the degree of emulsificationFatty-oils/fats productionWater bathsEnzymatic hydrolysis
The invention discloses a method for extracting tea oil by demulsification based on an aqueous enzymatic method. The method comprises the following steps: processing tea seed powder by microwaves for 60 to 120 seconds; adding distilled water at a material-liquid mass ratio of 1:4 to 1:8, pulping, and collecting upper-layer emulsified liquid; pulping the residual water phase and residues again, and collecting upper-layer emulsified liquid; combining the collected emulsified liquid; adding 0.5-6% of composite enzymatic hydrolysis liquid based on the total mass of the emulsified liquid, performing water bath enzymolysis, centrifuging, and taking upper-layer tea oil; adding an ethanol solution into the residual emulsified liquid, performing water bath treatment, centrifuging, and collecting upper-layer tea oil; combining the collected tea oil, an drying in vacuum. According to the method, the emulsified liquid is high in oil content, and the process is simple, novel and practical; the emulsified liquid is subjected to composite enzyme hydrolysis and ethanol demulsification, so that the enzymatic hydrolysis volume is greatly reduced, the enzyme consumption is greatly reduced, and the process is economic and efficient; microwaves, enzymatic hydrolysis and ethanol are adopted for assisting in oil extraction, so that natural active ingredients of the tea oil are better preserved, and the oil extraction process is safe and nutritive.
Owner:NANCHANG UNIV

Preparation method and technological system for enzymatically synthesizing N(2)-L-alanyl-L-glutamine

The invention provides a preparation method and technological system for enzymatically synthesizing N(2)-L-alanyl-L-glutamine. The preparation method comprises the steps of synthesis of L-alanine methyl ester, synthesis of N(2)-L-alanyl-L-glutamine, inactivation of enzymes, filtration, preparation of a crude product I of N(2)-L-alanyl-L-glutamine and preparation of a crude product II of N(2)-L-alanyl-L-glutamine. The technological system also comprises a production line formed by an L-alanine methyl ester synthesis kettle (1), an L-alanine methyl ester storage tank (2), an N(2)-L-alanyl-L-glutamine synthesis reaction kettle (3), an enzyme storage tank (4), a storage tank (5), a resin column (6) and mother liquor recovery tanks (7). The preparation method and the technological system have the technical effects that impurities are effectively removed, thus improving the purity of a finished product; the requirements for equipment are simple; the yield is high, after-treatment is simple to carry out, and the cost is low.
Owner:JIANGSU CHENGXIN PHARMA

Preparation method of egg white peptide

The invention discloses a preparation method of egg white peptide. The method comprises steps of: (1) taking egg white, homogenizing and diluting to prepare an enzymolysis stock solution; (2) sending the enzymolysis stock solution circularly into a reactor fixed with protease for an enzymolysis reaction for 0.5-1 h; continuously feeding an enzymolysis solution into a ceramic membrane filter for filtering; and returning a concentrated solution to the reactor for continuous enzymolysis; and (3) collecting a filtrate, and conducting spray drying to obtain an egg white peptide powder. The method provided by the invention can be conducted continuously, and has advantages of easily controllable reaction conditions, stable quality and performance of a final product, simple equipment, small amount of enzyme, low cost and easy popularization.
Owner:浙江艾格生物科技股份有限公司

Enzyme for producing sea cucumber oligopeptide and enzymolysis technology

InactiveCN106282284AEnzyme hydrolysis time is shortHigh yield of enzymatic hydrolysisPancreatinFermentationProteaseSea cucumber
The invention discloses an enzyme for producing sea cucumber oligopeptide and an enzymolysis technology, and belongs to the technical field of the sea cucumber oligopeptide production technology. On the basis of selecting a single enzyme, a special compound protease for sea cucumber enzymolysis comprising four enzymes such as incision enzymes and excision enzymes is formed by virtue of optimization; an enzyme activator is added into the compound protease; and after the temperature and PH are adjusted, sea cucumber slurry is preprocessed; and an appropriate amount of preprocessing agent is added before the preprocessing. The enzymolysis technology is reasonable in design, less in enzyme consumption, short in enzymolysis time, and high in enzymolysis yield. A product is good in taste, substantially no bitter or slightly bitter and little in fishy smell.
Owner:大连平岛天然产物科技有限公司

Method for preparing bananas powder with good recovery properties of water and non-agglomeration

The invention discloses a preparation method of banana powder with good rehydration characteristic and being not easy to agglomerate. The method makes banana flesh into the banana powder through procedures of color protection, thermal sterilization, controllable enzymatic hydrolysis technology, preparation, high speed shearing emulsification, secondary homogenization, high pressure spraying and drying, package with air-flow racking machine, and so on. Color protection with sodium bisulfite solution is carried out to the banana promptly after being peeled off skin, sterilization is made with hot water, then preparation is made by adding into VC, citric acid and water based on proportion, jordaning is carried out with a colloid mill after pulping, enzymatic hydrolysis is carried out with pectase, amylum, Beta-cyclodextrine, powdered sugar and water are carried out for a secondary preparation and a secondary homogenization, finally the banana powder is sprayed, dried and packed with aluminum-plastic promptly after being cooled. During the preparation process of the banana powder, no other filling agent is added except color protection agent and dispersant. The banana powder is characterized by good color, fluidity and rehydration characteristic, being easy to be stored and transported, etc.
Owner:SOUTH CHINA UNIV OF TECH

Preparation method of yolk globulin powder

The invention discloses a preparation method of a yolk globulin powder. The preparation method includes following steps: (1) dissolving yolk in deionized water, stirring the yolk and performing precipitation to extract a raw liquid; (2) performing salting-out precipitation to the raw liquid for removing residual fat, precipitating protein and adding water for reducing the protein, and performing plate-frame filtration to remove impurities for obtaining a clear liquid; (3) purifying the clear liquid through membrane separation for desalination and dehydration; and (4) performing micro-filtration for sterilization, and performing low-temperature spray drying to obtain the yolk globulin powder. The method can be carried out continuously. A production process is easy to control. A finally product is stable in quality and performance, is low in cost, can achieve industrial production and is easy to popularize.
Owner:浙江艾格生物科技股份有限公司

Method for continuous hydrolysis of cellobiose in straw by utilization of immobilized enzyme microreactor

The invention discloses a method for continuous hydrolysis of cellobiose in straw by the utilization of an immobilized enzyme microreactor. The method is characterized in that beta-glucosidase is immobilized in a microreactor and a substrate cellobiose aqueous solution is injected into the microreactor to carry out a micro reaction so as to degrade the cellobiose solution into glucose, wherein a carrier for enzyme immobilization is a quartz capillary tube and a coupling agent for enzyme immobilization is a toluene solution of (3-mercaptopropyl)trimethoxy silane. By the utilization of the microreactor, cellobiose in straw undergoes enzymatic hydrolysis to generate monosaccharide. The enzyme dosage is low, reaction efficiency is greatly raised, and energy consumption is reduced. By changing operation condition control, hydrolysis rate of cellobiose can be remarkably raised, and handling capability of straw is greatly enhanced. The device is a capillary holow tube, and the structure is simple. Only by simply amplifying number, production capacity can be increased proportionally. The method of the invention has a good industrial prospect.
Owner:NANJING UNIV OF TECH

Production method for preparing isomahooligosaccharide from waste residues of sweet potatoes

ActiveCN105838757ALow liquefaction temperatureReduce enzyme costFermentationChemistryMaltose
The invention discloses a production method for preparing isomahooligosaccharide from waste residues of sweet potatoes. The method includes: adding medium-temperature alpha-amylase into the waste residues of the sweet potatoes, heating, gelatinizing and liquefying; saccharifying by intrinsic beta-amylase in the waste residues of the sweet potatoes to obtain maltose; adding alpha-glucosyltransferase to realize glucoside conversion of the maltose to generate maltooligosaccharide. The production method has the advantages of simplification of technical process, low heat consumption and excellent targeting property by combination of existing production process of sweet potato starch processing enterprises and characteristics of fresh sweet potato residues, thereby being suitable for popularization in the sweet potato starch production enterprises.
Owner:SHANDONG FOOD & FERMENT IND RES & DESIGN INST

Aseptic long-acting enzymolysis method for plant protein

The invention relates to an aseptic long-acting enzymolysis method for plant protein, and belongs to the technical field of food processing. The method comprises the following steps: preparing ground vegetable protein raw materials or vegetable protein extracts into material liquid by water; making the pH of the material liquid become the optimum pH of protease, and carrying out sterilization; performing aseptic operation,preparingfood product protease into an enzyme solution with required concentration by water, filtering and performing sterilization, adding the filtered and sterilizedenzyme solution to the sterilizedmaterial liquid, thus obtaining a reaction solution; stirring the reaction solution to be fully mixed, and carrying out enzymolysis at the optimum temperature; heating and carrying out enzyme denaturalixation. The method can improve the hydrolysis degree of the vegetable protein, prolong the enzymolysis time, greatly lower theusing amount of enzymes and obviously lower risks of bacterial contamination;the hydrolysis degree of the vegetable protein is more than 18%; the problems of difficultenzymolysis of the vegetable protein, large using amount of the enzymes and easy corruption of enzymolysis liquid during the enzymolysis process are effectively solved; the use cost of the enzymesis lowered; a preservative and an enzyme protection agent are not used, so that components and taste of hydrolysates are not affected, and the application of the hydrolysates is more meaningful.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY +1

Process for preparing micro-molecular fish scale collagen peptides

ActiveCN102154424BHigh yieldReduce the effect of enzyme activityPeptide preparation methodsFermentationFish processingCollagenan
The invention discloses a process for preparing micro-molecular fish scale collagen peptides and belongs to the technical field of biological extraction. The process comprises the following steps of: 1, pretreating fish scales, and removing impurities and fat on the surfaces of fish scales; 2, extracting fish scale collagen peptides by using a high-temperature sulfuric acid extraction method, wherein the extraction yield is over 95 percent; 3, purifying the extracting solution by adopting the process technology combining centrifugal separation and membrane separation to obtain macro-molecularfish scale collagen peptides of which the purity is over 90 percent; 4, hydrolyzing the macro-molecular fish scale collagen peptides by using protease, and performing enzymic inactivation to obtain the solution of micro-molecular fish scale collagen peptides of which the purity is more than 90 percent and the relative molecular weight is less than 1.5kD; and 5, spray-drying and quickly drying thesolution of collagen peptides to obtain the finished product of micro-molecular fish scale collagen peptides. The micro-molecular fish scale collagen peptides prepared by the process have the advantages of low cost, high yield, high purity and low molecular weight; moreover, in the process, the fish scales discarded in fish processing are fully utilized, and the wastes are changed into the valuables.
Owner:THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION

Method for integrated extraction of plurality of biologically active ingredients in soft-shelled turtle

The invention discloses a method for integrated extraction of a plurality of biologically active ingredients in a soft-shelled turtle. The method comprises the following steps: S1, adding a complex protease A into a soft-shelled turtle crushed material liquid for enzymolysis; S2, filtering the soft-shelled turtle enzymolysis product, separating soft-shelled turtle crushed bones and a soft-shelledturtle meat liquid product, carrying out centrifugation to obtain a water-phase product of soft-shelled turtle meat and an oil-phase product of the soft-shelled turtle meat; S3, removing the fishy smell of the water-phase product of soft-shelled turtle meat, and carrying out decoloration, fine filtering, concentration and drying to obtain the soft-shelled turtle meat oligopeptide powder; S4, steaming a soft-shelled turtle crushed bone liquid, carrying out filtering to obtain a bone residue and a crushed bone liquid product, carrying out centrifugation to obtain a protein solution of soft-shelled turtle bones and an oil-phase product of the soft-shelled turtle bones; S5, adding a complex protease B into the protein solution of the soft-shelled turtle bones for enzymolysis to obtain a soft-shelled turtle bone protein enzymolysis product; S6, removing the fishy smell of the soft-shelled turtle bone protein enzymolysis product, and carrying out decoloration, fine filtering, concentration and drying to obtain oligopeptide powder of the soft-shelled turtle bone protein; S7, treating the bone residue to obtain soft-shelled turtle shell powder; and S8, mixing the oil-phase product of the soft-shelled turtle meat and the oil-phase product of the soft-shelled turtle bones to obtain soft-shelled turtle oil. According to the method provided by the invention, the soft-shelled turtle resources can be fully utilized, and resource waste is avoided.
Owner:大连平岛天然产物科技有限公司

Method for performing on bagasse by using cellulase-containing multiple enzymes

The invention discloses a method for performing synergic enzymolysis on bagasse by using cellulase-containing multiple enzymes. The substrate enzymolysis is the core link for preparing cellulosic ethanol, and the enhancement of the cellulose enzymolysis rate and the reduction of the cellulase use cost are key factors for implementing cellulosic ethanol industrialization. The multienzyme composite is utilized to perform synergic enzymolysis on the bagasse cellulose. One or combination of several of xylanase, pectinase, laccase, manganese peroxidase and lignin peroxidase and the cellulase constitute the multienzyme composite, and the multienzyme composite is utilized to perform enzymolysis on bagasse under certain conditions. Compared with the traditional cellulose enzymolysis, the method solves the problems of low substrate enzymolysis rate, long enzymolysis cycle and high enzyme consumption in the cellulosic ethanol preparation process, and provides a new method for high-quality utilization of bagasse.
Owner:INNER MONGOLIA UNIVERSITY

Method for producing polypeptone by enzymolysis of casein as raw material

A method for producing polypeptone by enzymatic hydrolysis of casein as a raw material. The specific process steps include pretreatment, primary enzymatic hydrolysis, secondary enzymatic hydrolysis, refining, concentration, concentration, spray drying, and heating pretreatment to loosen the helical structure of casein At the same time, the enzymatic hydrolysis with compound protease is used twice, the amount of enzyme used is small, the enzymatic hydrolysis time is short, and the content of amino nitrogen increases to 5%-7%, which is economical and practical, and is suitable for popularization.
Owner:靳群牛

Buffer solution and application thereof

The invention discloses a buffer solution and application thereof. The buffer solution is prepared from a Tris buffer solution with a concentration of 33 to 66 mM, divalent cations with a concentration of 1.6 to 5 mM, monovalent cations with a concentration of 25 to 75 mM, dithiothreitol (DTT) with a concentration of 0.5 to 10 mM, PEG 4000 to 8000 with a concentration of 5 to 15% and ATP with a concentration of 0.5 to 2 mM. The pH value of the Tris buffer solution is 7 to 9. The buffer solution can improve the efficiency of a ligation reaction, increase the conversion rate of a substrate and reduce the mismatch rate of the ligation reaction, is good in compatibility and small in inhibition effect on ligase, and remarkably reduces the enzyme consumption of a system, so experiment cost is reduced.
Owner:ZHEJIANG ANNOROAD BIO TECH CO LTD +1

Preparation method of grease with humanized structure

The invention discloses a preparation method of grease with a humanized structure. The preparation method comprises the following steps: carrying out intra-molecular rearrangement reaction to palm oil with palmitic acid content of higher than 50% under the action of catalyst to obtain Sn-2 locus triglyceride with palmitic acid content of higher than 50%; and carrying out ester exchange reaction to the Sn-2 locus triglyceride with palmitic acid content of higher than 50% with mixed fatty acid or non-glyceride under the action of 1,3 locus Specificity lipase, and then subjecting the reaction product to after treatment, so as to obtain the grease with humanized structure, wherein the mixed fatty acid is mixture of at least two selected from the group consisting of decanoic acid, lauric acid, myristic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid and linolenic acid. The vegetable oils are used as the raw material according to the method, so the safety is high; the preparation process is simple, the reaction time is short, the enzyme dosage is low, and the cost is low; and the prepared grease with the humanized structure is very similar to the beast milk fat in the composition of fatty acid and the structural distribution of triglyceride, so the grease can be added in infant formula or formula foods and used as breast milk fat substitute.
Owner:ZANYU TECH GRP CO LTD

A kind of preparation method of pea peptide

The invention provides a method for preparing pea peptides with low bitterness, low enzyme consumption and high peptide yield, and belongs to the technical field of pea deep processing. In the present invention, the structure of pea protein is expanded through the pretreatment of high-pressure steam sterilization, which is more conducive to enzymolysis, and the bitterness value of the enzymolysis solution is greatly reduced through the compound enzymolysis of Alcalase protease and protease P, and the finally obtained peptide The powder has low bitterness, and the total enzyme amount is lower than that of the prior art. The preferred action pH value of protease P is similar to the preferred action pH value of Alcalase protease, which avoids the excessive increase of salt in the peptide powder, and the finally prepared peptide powder aqueous solution is close to neutral. The protein content of the pea peptide powder prepared by the method proposed by the invention is more than 90%, the peptide yield reaches 73%, and the generated free amino acid content is lower than 15%. The pea peptide powder prepared by the invention can be used for nutrition, health food and special medical formula food.
Owner:南京泛成生物科技有限公司

A kind of biological enzyme that catalyzes the synthesis of glutamic acid dipeptide and its preparation method and application

The invention provides a bio-enzyme for synthesis of proglumetacin dipeptide N-(2)-L-alanyl-L-glutamine by catalysis as well as a preparation method and an application thereof. The amino acid sequence of the bio-enzyme is shown as SEQID NO: 2. The preparation method of the bio-enzyme comprises the following steps: firstly, constructing a genetically engineered strain of the bio-enzyme, and recombining plasmids by cutting the gene segment of the bio-enzyme linearly by using a restriction endonuclease SalI, wherein the gene segment of the bio-enzyme is totally synthesized by genes; and then selecting a positive clone, adding the positive clone into a YPD liquid culture medium, activating, inoculating the positive clone in a BMGY liquid culture medium, culturing the positive clone until the OD reaches 1, inoculating the positive clone in 1% methanol, inducing for 72 hours to express the bio-enzyme, and further fermenting to obtain the bio-enzyme for synthesis of proglumetacin dipeptide N-(2)-L-alanyl-L-glutamine by catalysis. The synthesis of N-(2)-L-alanyl-L-glutamine from bio-enzyme by catalysis is as follows: heating to deactivate, filtering, removing the generated salt by adopting a nanofiltration technology, concentrating, and crystallizing with methanol and water to obtain the high-purity N-(2)-L-alanyl-L-glutamine. The synthesis method of N-(2)-L-alanyl-L-glutamine is simple, efficient and environment-friendly and has extremely high economic value and market competitiveness.
Owner:JIANGSU CHENGXIN PHARMA

Transaminase mutants and application thereof

The invention provides transaminase mutants and application thereof. The transaminase mutants are subjected to amino acid mutation on the basis of a sequence shown in SEQ ID NO: 1, and the amino acid mutation is single-point mutation or combined mutation of W60Y, Y168A, V379W, V379L, V379M, C418Q and C418W. The activity, stability, temperature tolerance, pH tolerance and organic solvent tolerance of the transaminase mutants are improved, the problem that transaminase is poor in tolerance in an extreme environment in the prior art is solved, and the transaminase mutants are suitable for the field of enzyme engineering.
Owner:ASYMCHEM LAB TIANJIN

Method for preparing seaweed dietary fibers via ultrasonic enzymolysis

The invention belongs to the field of food processing, and in particular relates to a method for preparing seaweed dietary fibers via ultrasonic enzymolysis. The method is characterized by comprising the following steps: carrying out heating and immersion cleaning, dewatering and drying, colloid pulping, ultrasonic enzymolysis, bleaching treatment, separation and alcohol leaching, vacuum drying and the like on seaweed residues as raw materials so as to obtain the seaweed dietary fibers. The production method which is simple in treatment process, easy to industrialize and low in production cost, can reduce the enzyme adding amount and ensures that the seaweed dietary fibers are safe to eat, is provided. Thus, the effective value-added utilization of the seaweed residues is realized. The method has high economic and social benefits.
Owner:TIANJIN HIMALAYA HEALTH TECH CO LTD

Fine purification technique for bletilla striata polyoses glue

ActiveCN101195839BViscosity is not affectedHigh viscosityFermentationBiotechnologyBletilla striata
The invention discloses a purification process of Bletilla striata polysaccharide, which belongs to the natural plant polysaccharide deep processing technology field. The process steps are as follows: crude polysaccharide from Bletilla strata is scrubbed with hot alcohol, water adding is performed for redissolution, protease adding is performed for enzymolysis, quenching enzyme and centrifugationare performed, supernatant is collected, ethanol adding is performed for precipitation, the scrubbing is performed with complex solution, and filtration, drying and storage are performed. The products produced through the process have high purity and good dissolution property, and therefore the application range of the products is broadened; the enzyme consumption of the process is small, the rawmaterial is obtained easily, the cost is low, and the industrialization production is feasible.
Owner:NANJING WILD PANT COMPREHENSIVE UTILIZATION INST THE ALL CHINA SUPPLY & MARKETING COOPERATIV

A kind of method using laccase to catalyze the synthesis of silibinin

The invention discloses a method for catalytically synthesizing silybin through laccase, and belongs to the field of biochemical industry. According to the method for catalytically synthesizing the silybin through the laccase, four types of isomerides of the silybin are synthesized by catalyzing coniferyl alcohol and taxifolin through the laccase. The reaction is conducted in a water phase, and use of organic solvent is avoided; the reaction efficiency is high, and 0.9694 mg / L of the silybin can be generated within 1 h. The method for synthesizing the silybin is safe and efficient.
Owner:JIANGNAN UNIV

Pulping process by using grasses as raw material

The invention relates to a pulping process by using grasses as a raw material, and belongs to the field of paper making. The pulping process comprises twice cooking steps, wherein the cooking liquid used in primary cooking is prepared from the following components in percentage by mass: 5-8 percent of sodium hydroxide, 0.5-1.0 percent of sodium sulfite, 1-2.5 percent of sodium sulfide, 0.3-0.5 percent of anthraquinone, 0.02-0.1 percent of sodium dodecyl sulfate and the balance of water; and the cooking liquid used in secondary cooking is a mixed enzyme liquid consisting of an enzyme liquid I and an enzyme liquid II in a mass ratio of (1-3):1. The process has small dosage of alkali and enzyme to reduce waste emission, and is environment-friendly since the mixed enzyme cooking liquid can beused repeatedly; and furthermore, the process has short cooking time and good cooking effect.
Owner:广西宏瑞泰纸浆有限责任公司

Method for extracting fucoidan for brown seaweed

The invention relates to a fucoidan extract method, in particular to a method for extracting fucoidan for brown seaweed, comprising the following steps: 1. desalting: adopting fresh brown seaweed or unfreezed frozen brown seaweed to soak in pure water to remove salt; 2. enzymolysis: adding enzyme in the desalted brown seaweed to perform enzymolysis at 40-60 DEG C for 1-3h; 3. abstraction: adding calcium chloride in enzymatic hydrolyzate obtained by enzymolysis, and heating the solution to 90-100 DEG C for 1-4h to obtain the fucoidan extract. The invention is characterized of simple operation,high application security, high extraction efficiency, high extraction purity and the like and the extraction process is applicable to factory mass production.
Owner:大连海宝生物技术有限公司

Buffers and their applications

The invention discloses a buffer and an application thereof, wherein the buffer comprises: 33-66mM Tris buffer, 1.6-5mM divalent cations, 25-75mM monovalent cations, 0.5-10mM dithiothreitol (DTT), 5‑15% PEG4000‑8000 and 0.5‑2mM ATP, pH 7‑9. The buffer can improve the efficiency of the ligation reaction, increase the conversion rate of the substrate, reduce the mismatch rate of the ligation reaction, and has good compatibility and little inhibitory effect on the ligase. At the same time, it can significantly reduce the amount of enzyme used in the system, thereby reducing the experimental the cost of.
Owner:ZHEJIANG ANNOROAD BIO TECH CO LTD +1

A kind of preparation method and process system of enzyme-catalyzed synthesis of glutamate dipeptide

The present invention provides an enzyme-catalyzed preparation method and a process system for synthesizing triglutide dipeptide, including synthesis of L-alanine methyl ester, triglutin dipeptide synthesis, enzyme inactivation, filtration, preparation of triglutin crude product 1, and triglutin crude product 2. The process system of the present invention also includes an L-alanine methyl ester synthesis kettle (1), an L-alanine methyl ester storage tank (2), a glutamate synthesis reaction kettle (3), an enzyme storage tank (4), and a storage tank (5), resin column (6), and mother liquor recovery tank (7). The invention effectively removes impurities, improves the purity of finished products, has simple equipment requirements, high yield, simple aftertreatment and low cost.
Owner:JIANGSU CHENGXIN PHARMA

Camellia oil compatible with green tea extract and preparation method thereof

The invention relates to camellia oil compatible with a green tea extract and a preparation method thereof. The camellia oil compatible with the green tea extract comprises the green tea extract and the camellia oil in mass ratio of 1:10-100. According to the invention, a colloid mill is used to fully destroy green tea cells by milling, and the green tea milk is obtained to fully extract tea polyphenols and fat-soluble vitamins A, K and E contained in green tea. The preparation method of the invention can achieve the effect of separating more tea fat-soluble substances through a simple centrifugal process. The preparation method of the invention separates tea emulsion with high oil content through high-speed centrifugation, and then carries out two-step demulsification including enzymolysis with compound enzyme and ethanol extraction on the emulsion. The preparation method achieves complete demulsification, smaller volume of the emulsion, great reduction of enzyme dosage, and simple and economic processes. According to the present invention, the microwave, compound enzymolysis and ethanol-assisted extraction are combined to extract the tea polyphenols and the fat-soluble vitamins A, E, K, etc., in green tea, thereby preserving natural active ingredients in the tea well. The extraction process is safe and keeps nutritional ingredients well.
Owner:湖南山润油茶科技发展有限公司
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