Preparation method of yolk globulin powder

A technology of egg yolk globulin powder and egg yolk, which is applied in the field of preparation of egg yolk globulin powder, can solve the problems of large consumption of reagents, unstable properties, and low recovery rate, and achieve easy control of reaction conditions, stable quality and performance, and simple equipment Effect

Inactive Publication Date: 2015-02-04
浙江艾格生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned methods generally have the defects of easily causing protein inactivation and denaturation during the production process, resulting in unstable properties, as well as the problems of many steps in the production process, small batches, large consumption of reagents, and low recovery rate, which are not conducive to technological production.

Method used

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  • Preparation method of yolk globulin powder
  • Preparation method of yolk globulin powder

Examples

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Embodiment 1

[0026] Take 4 kg of egg yolk, add 4 times the volume of deionized water, stir for 5 minutes, put it into the clarification tank, and let it stand for 4 hours to obtain the supernatant, extract the supernatant to the mixing tank, and the remaining egg yolk liquid can be used to prepare egg yolk powder; above The supernatant is in a stirring state, the stirring speed is 50-70r / min, add the saline solution according to the volume / weight ratio of 4:1, turn off the stirring when the saline solution is completely added, and let the supernatant stand for 3-4 hours to precipitate the protein . After the protein is completely precipitated, the upper liquid is extracted to remove fat. Add deionized water to reduce protein according to the ratio of upper liquid: deionized water volume ratio 1:5, and prepare for plate and frame filtration. Install the filter cloth on the plate and frame filter press. After pre-coating with diatomaceous earth, filter the reduced protein solution through t...

Embodiment 2

[0028] Take 20 kilograms of egg yolk, add 4 times the volume of deionized water, stir for 10 minutes, put it into a clarification tank, and let it stand for 6 hours to obtain a supernatant. After the supernatant is removed, the remaining egg yolk liquid is used to prepare egg yolk powder; add ammonium sulfate (sodium chloride) to a saturated concentration of 33%, stirring at a rate of 50-70 r / min for 5-10 min. When the salt solution is completely added, the stirring is turned off, and the supernatant is allowed to stand for 3 to 4 hours to precipitate the protein. After the protein is completely precipitated, the upper liquid is extracted to remove fat. Add deionized water to reduce protein according to the ratio of upper liquid: deionized water volume ratio 1:5, and prepare for plate and frame filtration. Install the filter cloth on the plate and frame filter press, after pre-coating with diatomite, filter the reduced protein solution through the plate and frame, the filtrat...

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Abstract

The invention discloses a preparation method of a yolk globulin powder. The preparation method includes following steps: (1) dissolving yolk in deionized water, stirring the yolk and performing precipitation to extract a raw liquid; (2) performing salting-out precipitation to the raw liquid for removing residual fat, precipitating protein and adding water for reducing the protein, and performing plate-frame filtration to remove impurities for obtaining a clear liquid; (3) purifying the clear liquid through membrane separation for desalination and dehydration; and (4) performing micro-filtration for sterilization, and performing low-temperature spray drying to obtain the yolk globulin powder. The method can be carried out continuously. A production process is easy to control. A finally product is stable in quality and performance, is low in cost, can achieve industrial production and is easy to popularize.

Description

technical field [0001] The invention relates to a preparation method of egg yolk globulin powder, in particular to a method for extracting egg yolk immunoglobulin, an active substance in egg yolk, by using a water dilution method combined with salting out and other processes, and preparing the egg yolk globulin powder through ultrafiltration purification. Background technique [0002] Poultry eggs are rich in biologically active substances and substances with nutritional value, especially the yolk solids content is about 50%, protein is about 16%, lipid is about 32%, inorganic matter and carbohydrates are 2% and 1% respectively. %. Most yolk proteins are phosphoproteins and lipoproteins, which can be roughly divided into low-density lipoproteins, ovoglobulins, phosphoproteins and high-density lipoproteins. At present, the development and utilization of egg yolk mainly focus on egg yolk, egg yolk lecithin and other components. There are also reports on the development of egg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/02C07K1/34
Inventor 姚云昕
Owner 浙江艾格生物科技股份有限公司
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