15 results about "Yolk Proteins" patented technology

The invention discloses a yolk protein source tripeptide with a function of improving osteoporosis and application of the yolk protein source tripeptide, and belongs to the technical field of biological medicines. The tripeptide is a tripeptide APW of which the amino acid sequence is Ala-Pro-Trp. The peptide fragment can be obtained through artificial synthesis or directional enzymolysis of defatted yolk powder. Functional verification shows that the tripeptide APW and the egg yolk protein hydrolysate containing the peptide fragment have the function of improving the bone mineral density, and in a zebra fish osteoporosis model, when the egg yolk protein hydrolysate is 20 mu g / mL, the bone mineral density is improved by 58%; when the tripeptide APW is 0.5 mu g / mL, the bone mineral density is increased by 23.4%; in a rat osteoporosis model, the bone mineral density of thighbone is increased by 3.39% after 10 mg / kg. D of egg yolk protein enzymatic hydrolysate is eaten. Moreover, the food-borne bioactive peptide is high in biological safety, so that the tripeptide APW and a yolk protein enzymolysis product containing the peptide fragment can be applied to preparation of medicines for treating osteoporosis or health-care foods for improving bone mineral density, and the tripeptide APW has a good market prospect and application potential.

The invention belongs to the technical field of biology, and particularly relates to a receptor peptidomimetic capable of improving the breeding efficiency of butter crabs and application of the receptor peptidomimetic. The optimal binding conformation is screened according to the sequence prediction binding conformation of the vitellogen and the receptor of the vitellogen, the hotspot residues are screened according to the optimal binding conformation, and the binding energy change before and after mutation of the hotspot residues is calculated to determine the receptor peptidomimetic sequence. The receptor peptidomimetic can obviously slow down the development speed of the ovary of the blue crab and effectively induce the formation of the butter crab by utilizing the specificity of specific recognition of the vitellogenin of the blue crab and the receptor, so that the butter crab product can be produced in a planned manner.

The application discloses a kind of based on water temperature illumination coupling regulation and control procambarus clarkii ovulation induction system, for the existing system There are many environmental factors, poor coordination ability, temperature monitoring precision is insufficient, lack of real-time perception of physiological state of shrimp problem.The system of the present application includes heat insulation workshop, induced ovulation pool, high-level reservoir, water temperature control module, illumination control module, oxygenation module, water quality monitoring module, microporous camera, dehumidification device, bottom improvement agent automatic feeding device, non-contact yolk protein fluorescence detection probe and intelligent auxiliary system, intelligent auxiliary system is connected with each module respectively Unified control.The present application realizes the time sequence coupling regulation and control of multiple factors such as water temperature, illumination, water level and humidity by coordinating each module through intelligent auxiliary system, and effectively improves the ovulation induction success rate and synchronism of procambarus clarkii by real-time perception of physiological state of shrimp.The present application is mainly used for artificial propagation and seedling scale production of procambarus clarkii.

The invention discloses a method for constructing an anti-spinetoram neoseiulus californicus gene and an ovary targeting peptide of the anti-spinetoram neoseiulus californicus gene. According to the invention, a segment of yolk protein targeted peptide binding region is identified in Neoseiulus californicus, and the yolk protein targeted peptide binding region and a Cas9 sequence are fused and constructed on a PET28a carrier, and the NcvgP2C-Cas9 protein is obtained through prokaryotic expression. NcvgP2C-Cas9 protein, sgNcnAChR alpha 6 and an endosome escape reagent are mixed and injected into female adult mites, NcnAChR alpha 6 gene large fragment deleted neoseiulus californicus offspring is obtained and shows a phenotype with 23.2 times of resistance to spinetoram, and the resistance of a knockout strain to the spinetoram belongs to an autosome partial recessive genetic mode. The invention provides a new method for the genetic improvement of the drug resistance of Neoseiulus californicus, and enhances the compatibility between biological control and pesticide treatment strategies.

The invention discloses a construction method and application of a grouper oocyte in-vitro vitellogenin Vtg intake system. The method comprises the following steps: (1) preparing fluorescently-labeled vitellogenin Vtg-FITC; and (2) constructing an oocyte in-vitro culture system. The invention further discloses an application of the in-vitro vitellogenin Vtg intake system of the oocyte of the grouper, which is constructed by the method, in researching the regulation effect of gonadotropin on the Vtg intake of the oocyte. According to the method, the egg yolk intake difference caused by ovum surface area difference of ovarian follicles with different sizes is reduced by using oocytes in the same period; according to the invention, a Vtg-FITC marker is adopted as a means for quantifying the quantity of Vtg ingested by oocytes; according to the invention, the effect of gonadotropin on regulating and controlling Vtg intake of oocytes is preliminarily studied through the established in-vitro vitellogenin Vtg intake system of the oocytes of groupers.

The present invention discloses a functional gel feed for Anthocidaris crassispina and a preparation method thereof. Among them, the mass fraction of 5-HT in the functional gel feed is 0.05-0.5‰, and the energy of the functional gel feed is 1,800-2,000 kcal / kg; after feeding the Anthocidaris crassispina with the functional gel feed for 20-40 days, the content of vitellin in the Anthocidaris crassispina is 400-497 mg / g; and the gonadosomatic index is 9-13%. On the one hand, the functional gel feed of the present invention has a balanced nutrition, is rich in various nutrients required for the growth of Anthocidaris crassispina, can meet the growth and development needs of Anthocidaris crassispina, has a high feed digestibility, can reduce fecal emissions, and reduce the pollution of aquaculture to the environment. On the other hand, the feed is in a gel state, has good palatability, and is easy for Anthocidaris crassispina to ingest. In addition, the functional gel feed of the present invention has good stability in water, is not easy to disperse, and can improve the utilization rate of the feed.

The invention relates to miRNA for preventing and treating chilo suppressalis and application of the miRNA in cultivation of insect-resistant rice. The invention verifies that overexpression of miRNA-989 in chilo suppressalis remarkably reduces vitellogenin deposition in oocytes, resulting in that the number of oocytes in nests is remarkably reduced, further miR-989 is integrated into a rice genome for overexpression based on an amiRNA technology, the resistance of miRNA-transferred rice to chilo suppressalis is verified, the safety and stability of miRNA-transferred rice are evaluated, and the miRNA-transferred rice has good application prospects. A new germplasm resource is provided for the prior art, and a new effective prevention and control strategy is provided for green prevention and control of agricultural pests.

The application discloses egg yolk protein osteogenic peptides and a preparation method thereof, and belongs to the fields of biological medicines, functional food or egg product processing technologies. The application adopts defatted egg yolk powder enzymolysis to prepare osteogenic active peptides, and the osteogenic active peptides are enriched and separated through macroporous resins; the preparation and enrichment process is suitable for large-scale production, and can promote transformation from laboratory achievements to industrial production; two osteogenic active peptides are separated and identified, wherein, DFDLPT has the function of promoting osteoblast proliferation activity, and FDIDPG has the function of promoting osteoblast differentiation and mineralization.

The invention belongs to the technical field of biology, and particularly relates to a small molecule peptide for inhibiting development of ovaries of blue crabs and application of the small molecule peptide. According to the method, the optimal binding conformation is screened according to the sequence prediction binding conformation of the vitellogen and the receptor of the vitellogen, the hot residue is screened according to the optimal binding conformation, and the binding energy change before and after mutation of the hot residue is calculated to determine the competitive inhibition polypeptide sequence. The competitive inhibition polypeptide can obviously slow down the development speed of the ovary of the blue crab and effectively induce the formation of the butter crab by utilizing the specificity of specific recognition of the vitellogenin of the blue crab and a receptor, so that the butter crab product can be produced in a planned manner.

This invention belongs to the field of insect rearing technology, specifically relating to a method for artificially rearing the entire life cycle of the spotted bug (Aegilops spp.), comprising the following steps: S1, placing spotted bug eggs in a culture dish and transferring them to an intelligent artificial climate chamber until the nymphs hatch; S2, transferring the spotted bug nymphs from the intelligent artificial climate chamber to a rearing cage, and feeding them a full-life-cycle feed daily; S3, after the adults emerge, pairing the male and female adults, and feeding them a full-life-cycle feed daily, transferring the eggs to a rearing tube after the female lays eggs. The full-life-cycle feed for spotted bugs is composed of the following components by mass fraction: 90%-95% legume feed and 5%-10% egg yolk protein-loaded enzyme preparation. This method avoids contamination from spotted bug excrement and other impurities, reduces the probability of mutation and mortality, and improves the efficiency of nutrient absorption by spotted bugs.

The application discloses an egg yolk protein-derived tripeptide with the function of improving osteoporosis and application thereof, and belongs to the technical field of biological medicines. The tripeptide is a tripeptide APW with an amino acid sequence of Ala-Pro-Trp. The peptide segment can be obtained by artificial synthesis or directional enzymolysis of defatted egg yolk powder. Function verification shows that the tripeptide APW and egg yolk protein enzymolysis products containing the peptide segment have the function of improving bone density. In a zebra fish osteoporosis model, when the egg yolk protein enzymolysis product is 20 μg / mL, the bone density is increased by 58%; when the tripeptide APW is 0.5 μg / mL, the bone density is increased by 23.4%; in a rat osteoporosis model, when 10 mg / kg.d of the egg yolk protein enzymolysis product is eaten, the bone density of the femur is increased by 3.39%. Moreover, the food-derived bioactive peptide has high biological safety, so the tripeptide APW and the egg yolk protein enzymolysis product containing the peptide segment can be applied to the preparation of medicines for treating osteoporosis or health care foods for improving bone density, and have good market prospects and application potential.

Methods of targeting a molecule of interest to the oocyte of an animal are shown. In an embodiment the method utilizes a receptor binding region of a yolk protein precursor. In embodiment the yolk protein precursor is a YP1 sequence, functional fragment of functional variant thereof. The targeting molecule is linked to the molecule of interest. The molecule of interest may be a molecule of a gene editing system, such as CRISPR / Cas and in an embodiment comprises Cas. The methods and composition are useful for targeting a molecule of interest to an animal, such as an invertebrate or insect.

Methods of targeting a molecule of interest to the oocyte of an animal are shown. In an embodiment the method utilizes a receptor binding region of a yolk protein precursor. In embodiment the yolk protein precursor is a YP1 sequence, functional fragment of functional variant thereof. The targeting molecule is linked to the molecule of interest. The molecule of interest may be a molecule of a gene editing system, such as CRISPR / Cas and in an embodiment comprises Cas. The methods and composition are useful for targeting a molecule of interest to an animal, such as an invertebrate or insect.

The present application relates to a kind of chilo suppressalis prevention and cure miRNA and its application in cultivating insect-resistant rice.The present application verifies that the overexpression of chilo suppressalis in vivo miRNA-989 significantly reduces the yolk protein deposition in oocyte, causes the number of oocyte in nest to be significantly reduced, further based on amiRNA technology, miR-989 is integrated into rice genome to carry out overexpression, it is verified that the resistance of the rice of the miRNA to chilo suppressalis, and the safety and stability of the rice of the miRNA are evaluated, which provides new germplasm resources for prior art, and provides a new effective prevention and control strategy for green prevention and control of agricultural pests.