49 results about "Yolk Proteins" patented technology

The invention discloses a freshwater shrimp vitellogenin Vg gene, an encoding protein and an application of the freshwater shrimp vitellogenin Vg gene. The full-length cDNA sequence of the Vg gene is cloned from hepatopancreas of the freshwater shrimp by a primer group provided by the invention, and the expression of a vitellogenin gene in the ovary and the liver can be silenced after the dsRNA of the gene is injected to the pericardial cavity of a female freshwater shrimp, so that the ovary development of the freshwater shrimp is remarkably delayed. The invention provides a new thought and an effective means for solving the sexual precocity phenomenon of the freshwater shrimp and culturing good varieties.

The present invention belongs to the field of life science. Said invention provides a regulatory controlling sequence expressed by zebra fish vitellogenin 1, (vtg 1) gene of sequence 1. Said invention utilizes the transgenic zebra fish experiment to show that said sequence has promoter activity in the zebra fish body interior, and can make downstream gene implement specific expression in liver under the induction of female sex hormone. By adopting the connection of zfvtg 1-1.7 sequence provided by said invention and reporter gene (fluorescin) the fluorescence can be observed in the living body interior of transgenic zebra fish so as to quickly and visually reflect expression of vtg 1.

The invention discloses a method for preparing major yolk protein from a coelomic fluid of sea cucumber. The specific processes comprise the steps of pretreatment of the coelomic fluid of the sea cucumber, low-temperature desalting by a dialysis method, extraction of the major yolk protein, redissolving, dialysis and drying. The by-product, namely the coelomic fluid of the sea cucumber, generatedduring processing of the sea cucumber is used as a raw material, so that the utilization rate of the by-product generated during processing of the sea cucumber is raised, the protein resource of the coelomic fluid of the sea cucumber is fully developed and utilized, meanwhile, the pollution of the by-product of the sea cucumber to the environment is reduced, and the method has great significance to development and utilization of the major yolk protein in the coelomic fluid of the sea cucumber. Compared with traditional protein precipitation methods, which are hard to achieve scale production,such as ammonium sulfate salting-out, ethanol precipitation and isoelectric precipitation, the method provided by the invention is mild in operation condition, and therefore denaturation of biomacromolecules is not liable to occur; the extraction rate is up to 70%; and the method provided by the invention is simple, meanwhile, a large number of samples can be treated at a time, assistance of complex instruments is not needed, and the method is suitable for large-scale production.

The invention relates to instant yolk protein powder containing biologically active substances, and a preparation method thereof. Special biologically active substances matching the yolk protein powder are beta-carotene and conjugated linoleic acid glycerides. The instant yolk protein powder overcomes the problems of poor flavor of supplementing beta-carotene and linoleic acid alone and deterioration of color and bioactivity due to unstable properties when the biologically active substances are processed into the powder. The preparation method combines an ultrasonic assisted enzymolysis technology and mixes degreased yolk protein with water to carry out secondary spray drying, and carries out creative thermal insulation treatment on the yolk protein powder and the biologically active substances, thereby solving the problems of poor solubility of conventional yolk protein powder and poor stability after being blended, forming a porous structure on the surface of yolk protein particle surfaces, and being beneficial to absorption of the biologically active substances.

The invention discloses a freshwater shrimp opsin gene, a protein encoded by the same and application. The nucleotide sequence of the gene is SEQ ID NO: 1, and the nucleotide sequence encoded by the gene is SEQ ID NO: 2. A molecular mechanism for regulation and control of key genes related to exogenous environmental factors to development of ovary of crustacean, and a theoretical basis is providedfor sexual precocity of freshwater shrimps; and (2), dsRNA which is synthesized according to the nucleotide sequence of the freshwater shrimp opsin gene is injected into the cardiocoelom of a freshwater shrimp, vitellogeningene expression in the ovary of the freshwater shrimp can be relieved effectively, and therefore, the freshwater shrimp opsin gene has quite important influences to developmentof the ovary of the freshwater shrimp.

The invention discloses mixed feed for promoting portunus trituberculatus ovarian development and vitellogenesis and a preparation method of the mixed feed. The mixed feed is characterized in that the mixed feed is prepared by mixing the following raw materials in parts by weight: 25 to 30 parts of fish meal, 20 to 25 parts of soybean meal, 5 to 10 parts of krill meal, 20 to 25 parts of squid viscera meal, 15 to 20 parts of flour, 3 to 5 parts of soya bean lecithin, 1 to 2 parts of fish oil, 0.3 to 0.5 parts of cholesterol, 0.3 to 0.5 parts of choline chloride, 1.5 to 2.5 parts of sodium alginate, 0.5 to 1.0 part of composite additive, 0.5 to 1.0 parts of vitamin mixture, 1.0 to 1.5 parts of mineral substance mixture, 1.0 to 1.5 parts of monocalcium phosphate and 25 to 30 parts of water, the mixed feed has the advantages that the survival rate of portunus trituberculatus is improved by 18 to 22 percent, the gonad index can be improved by 34 to 40 percent, the content of vitellogenin can be improved by 36 to 46 percent, and the molecule expression quantity of the vitellogenin can be improved by 60 to 80 percent.

The invention discloses a seaweed oil nano-pickering emulsion and oil powder stabilized by yolk protein peptide particles and a preparation method and application thereof. The method comprises the steps that defatted yolk protein powder is dispersed in water and trypsin is added for enzymolysis; cooling, centrifugation, dialysis and freeze-drying treatment are carried out after enzyme deactivationin boiling water bath to prepare yolk protein peptide particle powder; the yolk protein peptide powder is dissolved in water, and seaweed oil is added, mixed with the yolk protein peptide powder andstirred; high-pressure micro-jet treatment is carried out after pre-homogenization so as to obtain the nano-scale seaweed oil pickering emulsion stabilized by the yolk protein peptide particles; spraydrying treatment is carried out on the nano-emulsion to obtain an oil powder product rich in omega-3 unsaturated fatty acid. Process conditions are simple and mild, the raw materials are natural andsafe, and rapid and continuous production can be carried out. Yolk protein powder of waste yolk obtained after oil extraction is reused, and nano-pickering emulsion and oil powder ingredients rich inomega-3 unsaturated fatty acid are prepared and can be applied to functional foods, medicines and cosmetics.

The invention discloses a tea geometrid vitellogenin gene vtg, which has the nucleotide sequence shown by SEQ ID No.: 1. The invention also discloses a vtg gene encoded protein, which has the amino acid sequence shown by SEQ ID NO: 2. The invention also discloses an application of the vtg gene: the vtg gene can be used to control the reproductive capacity of female Lepidoptera pests.

The invention discloses a yolk biological protein liquid and a yolk biological protein skin repair dressing prepared from the yolk biological protein liquid. The yolk biological protein skin repair dressing is prepared from main components as follows: 1%-5% of multiple yolk protein, 0.1%-0.3% of an epidermal growth factor and the balance of a hyaluronic acid solution, wherein the multiple yolk protein is an egg yolk antibody obtained by performing injection immunity on healthy hens, taking eggs laid by the hens and performing separation and purification with one or more pathogens capable of causing skin infection used as immunogen. The multiple yolk protein can inactivate various pathogens causing skin infection in a targeted manner, promote operation wound recovery and effectively prevent bacterial infection and causes no irritation to human bodies, and the added epidermal growth factor can repair wounds, promote epidermal wound surfaces to restore quickly, moisten skin and eliminate winkles and scars, has better biocompatibility with the multiple yolk protein, causes no dependence and drug resistance, has no allergy and is safe and efficient.

The invention discloses an egg yolk protein powder product capable of being suspended stably in water and a preparation method thereof. By controlling supercritical CO2 parameters such as pressure and temperature, deoiled egg yolk powder containing 5-10% of egg yolk oil is prepared firstly, then the phospholipid proportion of the deoiled egg yolk powder is controlled through the low carbon alcohol extraction technology, the level of the phospholipid is decreased to 1.0-1.5%, and the egg yolk protein powder mainly containing livetin is obtained. Tests prove that according to the egg yolk protein powder with the phospholipid content being controlled to be 1.0-1.5%, the suspending performance and stability are both remarkably improved.

The invention discloses a preparation method of a yolk lipid-lowering peptide, which comprises the following steps: 1) carrying out enzymolysis, ultrafiltration and freeze-drying on yolk proteins to prepare a crude extract of the yolk lipid-lowering peptide; and 2) in order to obtain the refined product of the yolk lipid-lowering peptide, carrying out the following purification steps on the crude extract: carrying out freeze-drying on the crude extract of the porcine blood antimicrobial peptide, passing the crude extract through a gel-type cation exchange column, eluting the exchange column, collecting the eluate, and carrying out monomer preparation by using the high-performance counter-current chromatography eluate to obtain the yolk lipid-lowering peptide. The invention provides a new way and method for producing the lipid-lowering peptide.

The invention discloses a penaeus monodon female parent gonad maturation promoting small interfering RNA composition. The composition comprises small interfering RNA aiming at three hormone genes of penaeus monodon CHH, MIH and VIH for inhibiting gonad development, wherein the three small interfering RNAs are specifically CHH-siRNA, MIH-siRNA and VIH-siRNA, wherein the nucleotide sequence of a positive-sense strand of the CHH-siRNA is shown as SEQ ID NO. 1, and the nucleotide sequence of an antisense strand of the CHH-siRNA is shown as SEQ ID NO. 2; a positive-sense strand nucleotide sequenceof the MIH-siRNA is shown as SEQ ID NO. 3, and an antisense strand nucleotide sequence of the MIH-siRNA is shown as SEQ ID NO. 4; and the nucleotide sequence of the positive-sense strand of the VIH-siRNA is shown as SEQ ID NO. 5, and the nucleotide sequence of the antisense strand of the VIH-siRNA is shown as SEQ ID NO. 6. The invention also discloses a penaeus monodon female parent gonad maturation promoting test kit containing the small interfering RNA composition and a use method thereof. The small interfering RNA composition can remarkably reduce the content of hormone for inhibiting gonaddevelopment maturity of female parent shrimps, promote the generation of egg yolk protein and accelerate the gonad development process.

The invention discloses a yolk bioprotein foam filler which is mainly composed of 8-10% of multi-link yolk protein, 0.1-0.3% of preservative, 40-50% of matrix and the balance of a foamer.The multi-link yolk protein is yolk bioprotein capable of being combined with various pathogens, and the pathogens include, but not limited to ureaplasma urealyticum, mycoplasma hominis, staphylococcus, streptococcus, Escherichia coli, Gardnerella vaginalis, gonococcus and Tritirachium album.The yolk bioprotein foam filler is mainly composed of yolk bioprotein aiming at inhibiting pathogenic bacteria, can be combined with corresponding pathogens, can effectively inhibit and remove pathogens, is effective on most bacteria, mycete and mycoplasma, does not have impact on probiotics, can maintain health and physiological environment of a reproductive system, does not enter blood, is higher in safety during local use, is free of irritation and side effect on human body, dependency, drug resistance and hypersensitivity and can be used for various clinical therapeutic products for vaginitis.

The invention is directed to methods for extracting phosvitin from egg yolk involving contacting the egg yolk or egg yolk protein granules with a solution having a salt concentration of about 10% to form a mixture; optionally, heating the mixture; adjusting the pH of the mixture to separate phosvitin from other proteins; recovering the phosvitin. The phosvitin extract may be dephosphorylated and hydrolyzed to produce phosvitin phosphopeptides.

The present invention relates to a production method of synthetic egg powder lecithin. Said production method includes the following steps: using egg as raw material, separating egg white from egg yolk, respectively adding edible salt, hexane and ethyl alcohol so as to obtain extracted lecithin egg yolk oil, egg yolk protein, egg yolk powder residue and dried protein product, mixing them to obtain synthetic egg powder lecithin, more adding cream perfume, plant cellulose and sweetening agent so as to obtain synthetic egg powder lecithin health-care product.

The invention discloses a preparation method of major yolk proteins of sea urchins. The preparation method of the major yolk proteins of sea urchins takes yolk of sea urchins as a raw material, and comprises the following steps: preparing skimmed sea urchin yolk powder; carrying out alkali dissolving, acid precipitating and low-temperature desalinating by performing dialysis; and carrying out freeze-drying so as to obtain the main yolk proteins. By utilizing the yolk of sea urchins as the raw material, the preparation method of the major yolk proteins of sea urchins improves processing and utilization rates of the sea urchin yolk; and thus, protein resources of the sea urchin yolk are fully developed and utilized. The skimmed sea urchin yolk powder is prepared by adopting food-grade organic reagents n-hexane and anhydrous alcohol, so that, the operation is convenient and safe; moreover, the preparation steps are simple and effective. The main yolk proteins in the skimmed sea urchin yolk powder are dissolved and precipitated by using a sodium hydroxide solution and a hydrochloric acid solution; and then, neutralization reaction and dialysis desalination are performed, so that, application of the obtained major yolk proteins of sea urchins as a functional base material in foods can be guaranteed. Thus, the preparation method is of great significance to development and utilizationof sea urchin yolk resources.

The invention discloses a method for extracting main egg yolk protein of sea cucumber body walls by three-step precipitation, which belongs to the field of protein extraction, and comprises the following steps: preparing sea cucumber body wall protein crude extract by using sea cucumber body walls as raw materials, carrying out acid precipitation on mixed acetone precipitated protein, removing impure protein by Sevage, precipitating target protein by ammonium sulfate, performing desalting at low temperature by a dialysis method, carrying out freeze drying, obtaining the main egg yolk protein with high purity, wherein the main egg yolk protein of the protein is identified as a glyphosate protein through the cooperation of mass spectrometry and electrophoresis technologies. According to theinvention, high-valued utilization of sea cucumbers is improved, protein resources are fully developed and utilized, and a prerequisite basis is provided for research of protein targeting functional activity; the whole process is simple in experimental operation, the protein extraction rate and purity are high, special advanced instrument assistance is not needed, target protein is precipitated and separated through three steps, a large amount of main egg yolk protein can be obtained at a time, and the method is suitable for large-scale and high-precision purity research. The protein identification specificity is high, results are clear and visual, and operation is easy and convenient.

The invention provides a method for extracting a refined egg yolk antibody by utilizing chick infectious bursal disease virus. According to the technical scheme, low ion strength and specific acid condition of octanoic acid are maintained, so that octanoic acid can react with most of egg yolk protein and irreversible precipitate is formed; meanwhile, only IgY antibody protein is unreacted and is still left in liquid, so that egg yolk liquid is diluted and then is subjected to treatment by virtue of the octanoic acid, then solid-liquid separation is carried out on the egg yolk liquid treated by the octanoic acid, IgY aqueous solution with the purity being more than 80% can be obtained, the method for extracting the refined egg yolk antibody is economic, simple and rapid, antibody activity is not damaged, and recovery rate is high.

The invention discloses high-nutrition protein powder rich in natural calcium, iron and zinc. The high-nutrition protein powder rich in natural calcium, iron and zinc consists of the following components in percentages by weight: 10-80 parts of yolk protein powder, 10-80 parts of plant protein powder and 10-80 parts of animal protein powder. The high-nutrition protein powder contains natural calcium, iron and zinc, and can provide natural and healthful calcium, iron and zinc sources. The yolk protein powder in the high-nutrition protein powder contains natural lecithin, rough taste and poor solubility of the plant protein powder can be improved, the protein powder can be dispersed uniformly in the water and tastes smooth, and the appetite is enhanced. The high-nutrition protein powder has light saline taste and flavor, the shortcomings that soybean isolate protein powder, whey protein powder and the like are tasteless, and the appetite of users is poor are overcome, and crowds who want to supplement protein like the product. The high-nutrition protein powder contains the yolk protein powder with effect of removing cholesterol and fat, and provides high-quality protein for crowds with allergies on milk.

The invention provides a method for jointly extracting multiple kinds of yolk protein based on water phase separation and a product. The method includes the steps that (1), fresh yolk is taken, a water phase solution with the volume 0.5-5 times that of the yolk is added for dilution, and the material is stirred, and is subjected to standing or is centrifugally layered; an upper-layer component A,a middle-layer component B and a lower-layer component C are obtained, wherein the water phase solution is polysaccharide matter with the mass percentage concentration of 0.1-0.5% or 2-8% of a water solution of low-molecular polymers; (2), the component A is taken, and yolk low-density lipoprotein is prepared; (3), the component B is taken, and yolk immune globulin is prepared; (4), the componentC is taken, and phosvitin and yolk high-density lipoprotein are prepared. The process is simple and short, the operation is easy, four kinds of yolk protein can be jointly extracted, the extracting efficiency is high, and the unit raw material output is high; extraction is carried out based on water phase separation, reagents used in the method are all food-grade or medicine-grade components, andthe reagents are green and safe; the treatment process is mild, and the functional characteristics and the bio-activity of the protein can be reserved to the maximum degree.

A recombinant expression and purification method of vitellogenin from bostrichthys sinensis belongs to the technical field of fusion protein expression and purification method. The method of the invention comprises the following steps: providing a vitellogenin gene based on bostrichthys sinensis, cloning part of the gene, recombining plasmids in vitro, transferring to escherichia coli for prokaryote expression, obtaining high-efficiency fusion expression at 16 DEG C of IPTG induction temperature, wherein the expressed target protein exists in a soluble state and accounts for more than 25% of total of bacterial soluble protein; applying a purification technology to obtain a fusion protein MNP-VTG, and separating a label MNP from the fusion protein to obtain a target protein VTG monomer through sucrose digestion. The method of the invention has high-efficiency expression, simple purification of VTG, more simple separation than inclusion bodies due to solubility and no need for renaturation. The obtained recombinant VTG provides stable and uniform raw material basis for subsequent applications. The recombinant protein can be used for biological activity function researches, and made into corresponding antibodies for detection research.

The invention discloses a promoter gene of cloned Chinese Anopheles vitellogenin (Vg) and Chinese Anopheles defending agent coding gene through molecular biological checking method, which is characterized by the following: constructing carrier plasmid; infecting Chinese Anopheles egg through microscopic injection; hatching the egg; observing GFP expression condition and RT-PCR checking result under fluorescent microscope; certifying excellent expression of Vg promoter to drive Defensin gene in gene-transmission Chinese Anopheles body; constructing gene-transmission system of Chinese Anopheles.

The invention discloses a zebra fish vitellogenin indirect competition ELISA kit and a detection method and application of the kit. The method comprises the following steps: respectively purifying lipovitellin and vitellogenin from zebra fish ovary and 17beta-estradiol exposed zebra fish body by using a method for combining two-step chromatography and ultrafiltration, and preparing rabbit anti-zebra fish vitellogenin polyclonal antibody; respectively filling a pure zebra fish lipovitellin product, the rabbit anti-zebra fish vitellogenin polyclonal antibody, a blank 96-pore ELISA plate, a coating solution, a washing solution, a blocking solution, sample diluent, a color developing solution, a stopping solution and a horse radish peroxidase labeled goat anti-rabbit second antibody into a box body, thereby obtaining the indirect competition ELISA kit for detecting the zebra fish vitellogenin. The kit disclosed by the invention can be applied to investigation of environmental estrogen substances, the vitellogenin in zebra fish plasma, overall homogenate, liver tissues and hepatic cell culture solution can be sensitively, accurately, conveniently and quantitatively detected, the detection range is 31.25-1000ng mL<-1>, and an important tool is provided for the application of the zebra fish in the field of endocrine disrupter detection.

The present invention relates to the gene, protein, polyclonal antibody and application of the vitellogenin of the spiny sea plate, and belongs to the field of molecular biology. The gene sequence of the spinous sea plate vitellogenin is SEQ ID NO: 1, and the protein is as follows: Described in SEQ ID NO:2. Utilize the ELISA method of the Vg antibody of the spiny carp Vg, by identifying the Vg content in the organism, and then warn the pollution status of EEs in the marine ecosystem, the detection kit prepared by using the spiny spiny Vg antibody can detect a certain The degree of estrogen pollution in the sea area is accurate and reliable.

The method for interspecific cross-breeding of charred trout with the male parent of charred trout belongs to the technical field of aquaculture. Broodstock breeding is carried out under different water temperature conditions, so that two fishes with different reproductive periods mature synchronously, hold eggs through isotonic fluid, fully stir to dissolve the yolk protein of broken eggs, carry eggs for insemination, and hatch fertilized eggs by holding eggs in a bucket and basket , FRP parallel tank hatch eye eggs. In particular, the cultivation of the male red salmon at low temperature not only prolongs the maturation time, so that it matures synchronously with the white salmon or American char, but also, at low temperature, the semen quality is good, the duration is long, and the breeding time is long. Interbreeding effect is good. By the method of the present invention, the biological traits of part of the red salmon are obtained, the muscle quality is similar to that of the red salmon, the growth rate is 50% to 60% faster than that of the red salmon, and the disease resistance is strong, which improves the breeding efficiency and benefit.

The invention discloses polypeptides and nucleic acids that mediate the endocytosis of exogenous proteins and their application, and a method for gene editing in Neocaridina heteropoda. The invention finds a length of A polypeptide of only 40aa, the polypeptide has an unexpected effect of mediating the endocytosis of exogenous proteins, so it is easy to express, purify, form RNP complexes and be endocytosed, and has a good effect in the gene editing of Neopodia prawn Application prospects. This technology can be widely applied to non-model organisms that are not yet proficient in embryonic microinjection technology, and is of great significance for Cas9-mediated gene editing.

The toothpaste containing the helicobacter pylori specific yolk protein and the natural plant extract, provided by the invention, has a stronger antibacterial effect while cleaning teeth.

The invention discloses a method for preparing main yolk protein from sea cucumber body cavity fluid. The specific process includes: pretreatment of sea cucumber body cavity fluid, low-temperature desalination by dialysis, extraction of main yolk protein, reconstitution, dialysis and drying. The present invention uses sea cucumber coelom fluid, a by-product of the sea cucumber processing process, as a raw material, improves the utilization rate of the by-product in the sea cucumber processing process, makes its protein resources fully developed and utilized, and reduces the pollution of sea cucumber by-products to the environment at the same time. The development and utilization of the main vitellin in body cavity fluid is of great significance. Compared with traditional protein precipitation methods such as ammonium sulfate salting out, ethanol precipitation, isoelectric point precipitation and other methods that are not easy to produce on a large scale, the present invention has mild operating conditions and is less likely to cause denaturation of biological macromolecules. And the extraction rate is as high as 70%. The method of the invention is simple, and can process a large number of samples at one time without the assistance of complex instruments, and is suitable for large-scale production.

A bone-strengthening composition of the invention comprises as an active ingredient an egg-yolk protein hydrolysate in which a proportion of the area of the region of a molecular weight of 500 or more and 20,000 or less to the total proportion of the area of proteins, peptides and amino acids is 50% or higher in a molecular weight distribution analysis by gel filtration chromatography. The composition has an action of osteoblast growth promotion, bone growth and bone strengthening, is effective in the prevention and treatment of a variety of bone diseases such as osteoporosis, and is useful as a safe and inexpensive product that can be widely used for a food and drink, a pharmaceutical or a feed and can be taken daily and continuously. The egg-yolk protein hydrolysate is preferably a hydrolysate obtained by defatting egg yolk with an organic solvent and hydrolyzing the resulting powder with a protein hydrolyzing enzyme.