22 results about "Yolk Proteins" patented technology

The invention discloses a yolk protein source tripeptide with a function of improving osteoporosis and application of the yolk protein source tripeptide, and belongs to the technical field of biological medicines. The tripeptide is a tripeptide APW of which the amino acid sequence is Ala-Pro-Trp. The peptide fragment can be obtained through artificial synthesis or directional enzymolysis of defatted yolk powder. Functional verification shows that the tripeptide APW and the egg yolk protein hydrolysate containing the peptide fragment have the function of improving the bone mineral density, and in a zebra fish osteoporosis model, when the egg yolk protein hydrolysate is 20 mu g / mL, the bone mineral density is improved by 58%; when the tripeptide APW is 0.5 mu g / mL, the bone mineral density is increased by 23.4%; in a rat osteoporosis model, the bone mineral density of thighbone is increased by 3.39% after 10 mg / kg. D of egg yolk protein enzymatic hydrolysate is eaten. Moreover, the food-borne bioactive peptide is high in biological safety, so that the tripeptide APW and a yolk protein enzymolysis product containing the peptide fragment can be applied to preparation of medicines for treating osteoporosis or health-care foods for improving bone mineral density, and the tripeptide APW has a good market prospect and application potential.

The invention belongs to the technical field of biology, and particularly relates to a receptor peptidomimetic capable of improving the breeding efficiency of butter crabs and application of the receptor peptidomimetic. The optimal binding conformation is screened according to the sequence prediction binding conformation of the vitellogen and the receptor of the vitellogen, the hotspot residues are screened according to the optimal binding conformation, and the binding energy change before and after mutation of the hotspot residues is calculated to determine the receptor peptidomimetic sequence. The receptor peptidomimetic can obviously slow down the development speed of the ovary of the blue crab and effectively induce the formation of the butter crab by utilizing the specificity of specific recognition of the vitellogenin of the blue crab and the receptor, so that the butter crab product can be produced in a planned manner.

The invention discloses a functional gel feed for purple sea urchins and a preparation method of the functional gel feed. Wherein the mass fraction of the 5-HT in the functional gel feed is 0.05%-0.05%, and the energy of the functional gel feed is 1800-2000 kcal / kg; after the functional gel feed is used for feeding the purple sea urchins for 20-40 days, the egg yolk protein content of the purple sea urchins is 400-497 mg / g; and the gonad index is 9-13%. On one hand, the functional gel feed disclosed by the invention is balanced in nutrition, rich in various nutrient substances required by the growth of the purple sea urchins, capable of meeting the growth and development requirements of the purple sea urchins, high in feed digestibility and capable of reducing excrement discharge and reducing pollution of breeding to the environment; and on the other hand, the feed is gel-shaped, is good in palatability and is easy to eat by the purple sea urchins. In addition, the functional gel feed is good in stability in water and not prone to loss, and the utilization rate of the feed can be increased.

The application discloses a kind of based on water temperature illumination coupling regulation and control procambarus clarkii ovulation induction system, for the existing system There are many environmental factors, poor coordination ability, temperature monitoring precision is insufficient, lack of real-time perception of physiological state of shrimp problem.The system of the present application includes heat insulation workshop, induced ovulation pool, high-level reservoir, water temperature control module, illumination control module, oxygenation module, water quality monitoring module, microporous camera, dehumidification device, bottom improvement agent automatic feeding device, non-contact yolk protein fluorescence detection probe and intelligent auxiliary system, intelligent auxiliary system is connected with each module respectively Unified control.The present application realizes the time sequence coupling regulation and control of multiple factors such as water temperature, illumination, water level and humidity by coordinating each module through intelligent auxiliary system, and effectively improves the ovulation induction success rate and synchronism of procambarus clarkii by real-time perception of physiological state of shrimp.The present application is mainly used for artificial propagation and seedling scale production of procambarus clarkii.

The invention discloses a method for constructing an anti-spinetoram neoseiulus californicus gene and an ovary targeting peptide of the anti-spinetoram neoseiulus californicus gene. According to the invention, a segment of yolk protein targeted peptide binding region is identified in Neoseiulus californicus, and the yolk protein targeted peptide binding region and a Cas9 sequence are fused and constructed on a PET28a carrier, and the NcvgP2C-Cas9 protein is obtained through prokaryotic expression. NcvgP2C-Cas9 protein, sgNcnAChR alpha 6 and an endosome escape reagent are mixed and injected into female adult mites, NcnAChR alpha 6 gene large fragment deleted neoseiulus californicus offspring is obtained and shows a phenotype with 23.2 times of resistance to spinetoram, and the resistance of a knockout strain to the spinetoram belongs to an autosome partial recessive genetic mode. The invention provides a new method for the genetic improvement of the drug resistance of Neoseiulus californicus, and enhances the compatibility between biological control and pesticide treatment strategies.

The invention discloses a construction method and application of a grouper oocyte in-vitro vitellogenin Vtg intake system. The method comprises the following steps: (1) preparing fluorescently-labeled vitellogenin Vtg-FITC; and (2) constructing an oocyte in-vitro culture system. The invention further discloses an application of the in-vitro vitellogenin Vtg intake system of the oocyte of the grouper, which is constructed by the method, in researching the regulation effect of gonadotropin on the Vtg intake of the oocyte. According to the method, the egg yolk intake difference caused by ovum surface area difference of ovarian follicles with different sizes is reduced by using oocytes in the same period; according to the invention, a Vtg-FITC marker is adopted as a means for quantifying the quantity of Vtg ingested by oocytes; according to the invention, the effect of gonadotropin on regulating and controlling Vtg intake of oocytes is preliminarily studied through the established in-vitro vitellogenin Vtg intake system of the oocytes of groupers.

The invention discloses an extraction method of artemia cyst yellow protein and application of artemia cyst yellow protein to zebra fish wound repair activity, and belongs to the field of biological medicine. The method comprises the following steps: destroying shells of artemia by using a dilute alkali urea composite solution, extracting yolk protein from artemia bodies by adopting an isoelectric precipitation method, and identifying that the protein is glycoprotein containing phospholipid through specific staining and a high-resolution mass spectrometry technology. A further research proves that the artemia cyst flavin can promote damage repair of zebra fish tissues through a zebra fish tail fin wound repair model. The effect is mainly realized by promoting secretion of inflammatory factors and exerting a tissue oxidative damage free radical scavenging function. The research not only discloses the biological activity of the artemia cyst yellow protein, but also expands the application range of the artemia cyst yellow protein in the field of aquaculture.

The invention belongs to the technical field of dried eggs, and particularly relates to dried eggs prepared on the basis of egg protein-sodium hyaluronate conjugates and a preparation method, and the dried eggs comprise the following components in parts by weight: egg white, the egg protein-sodium hyaluronate conjugates and auxiliary materials, the preparation method of the egg protein-sodium hyaluronate conjugate comprises the following steps: hydrating yolk protein, ovalbumin and sodium hyaluronate, carrying out glycosylation treatment, cooling, drying, storing and the like, and the product has high solubility, emulsibility, foamability and thermal stability. The glycosylated and modified egg protein is then used for preparing the dried egg, the yield, hardness, color and luster and water-holding capacity of the dried egg are remarkably improved, the edible quality of the dried egg is improved, and theoretical and technical supports are provided for widening the application range of sodium hyaluronate in processing food (such as egg products).

The present invention discloses a method for evaluating the effect of hormone-induced ovarian development of Japanese eels. The method comprises the following steps: S1: selecting female Japanese eels, providing non-denatured mammalian protein extract, FITC, and hCG. S2: obtaining serum containing vitellogenin. S3: obtaining purified vitellogenin. S4: coupling labeling treatment, verifying the purification effect. S5: setting up experiments to verify whether the target hormone can promote vitellogenin to enter oocytes, thereby effectively inducing ovarian development of Japanese eels. S6: observing the fluorescence intensity of the experimental group under a fluorescence microscope, verifying whether the target hormone can promote vitellogenin to enter oocytes based on Western Blot, and evaluating the induced development effect. The present invention is based on judging whether the target hormone can promote vitellogenin to enter oocytes, thereby effectively inducing ovarian development of Japanese eels, and is of great significance for accurately evaluating the effect of a new ripening agent on inducing yolk accumulation, thereby providing technical support for studying artificial breeding of Japanese eels.

The present invention discloses a functional gel feed for Anthocidaris crassispina and a preparation method thereof. Among them, the mass fraction of 5-HT in the functional gel feed is 0.05-0.5‰, and the energy of the functional gel feed is 1,800-2,000 kcal / kg; after feeding the Anthocidaris crassispina with the functional gel feed for 20-40 days, the content of vitellin in the Anthocidaris crassispina is 400-497 mg / g; and the gonadosomatic index is 9-13%. On the one hand, the functional gel feed of the present invention has a balanced nutrition, is rich in various nutrients required for the growth of Anthocidaris crassispina, can meet the growth and development needs of Anthocidaris crassispina, has a high feed digestibility, can reduce fecal emissions, and reduce the pollution of aquaculture to the environment. On the other hand, the feed is in a gel state, has good palatability, and is easy for Anthocidaris crassispina to ingest. In addition, the functional gel feed of the present invention has good stability in water, is not easy to disperse, and can improve the utilization rate of the feed.

The invention relates to miRNA for preventing and treating chilo suppressalis and application of the miRNA in cultivation of insect-resistant rice. The invention verifies that overexpression of miRNA-989 in chilo suppressalis remarkably reduces vitellogenin deposition in oocytes, resulting in that the number of oocytes in nests is remarkably reduced, further miR-989 is integrated into a rice genome for overexpression based on an amiRNA technology, the resistance of miRNA-transferred rice to chilo suppressalis is verified, the safety and stability of miRNA-transferred rice are evaluated, and the miRNA-transferred rice has good application prospects. A new germplasm resource is provided for the prior art, and a new effective prevention and control strategy is provided for green prevention and control of agricultural pests.

The application discloses egg yolk protein osteogenic peptides and a preparation method thereof, and belongs to the fields of biological medicines, functional food or egg product processing technologies. The application adopts defatted egg yolk powder enzymolysis to prepare osteogenic active peptides, and the osteogenic active peptides are enriched and separated through macroporous resins; the preparation and enrichment process is suitable for large-scale production, and can promote transformation from laboratory achievements to industrial production; two osteogenic active peptides are separated and identified, wherein, DFDLPT has the function of promoting osteoblast proliferation activity, and FDIDPG has the function of promoting osteoblast differentiation and mineralization.

The invention belongs to the technical field of biology, and particularly relates to a small molecule peptide for inhibiting development of ovaries of blue crabs and application of the small molecule peptide. According to the method, the optimal binding conformation is screened according to the sequence prediction binding conformation of the vitellogen and the receptor of the vitellogen, the hot residue is screened according to the optimal binding conformation, and the binding energy change before and after mutation of the hot residue is calculated to determine the competitive inhibition polypeptide sequence. The competitive inhibition polypeptide can obviously slow down the development speed of the ovary of the blue crab and effectively induce the formation of the butter crab by utilizing the specificity of specific recognition of the vitellogenin of the blue crab and a receptor, so that the butter crab product can be produced in a planned manner.

This invention belongs to the field of insect rearing technology, specifically relating to a method for artificially rearing the entire life cycle of the spotted bug (Aegilops spp.), comprising the following steps: S1, placing spotted bug eggs in a culture dish and transferring them to an intelligent artificial climate chamber until the nymphs hatch; S2, transferring the spotted bug nymphs from the intelligent artificial climate chamber to a rearing cage, and feeding them a full-life-cycle feed daily; S3, after the adults emerge, pairing the male and female adults, and feeding them a full-life-cycle feed daily, transferring the eggs to a rearing tube after the female lays eggs. The full-life-cycle feed for spotted bugs is composed of the following components by mass fraction: 90%-95% legume feed and 5%-10% egg yolk protein-loaded enzyme preparation. This method avoids contamination from spotted bug excrement and other impurities, reduces the probability of mutation and mortality, and improves the efficiency of nutrient absorption by spotted bugs.

According to the method for fattening and spawning induction before spawning induction, terrestrial cabbages are adopted as one of main bait components of the purple urchins, laver and mussels are combined, and according to parent characteristics of the purple urchins before spawning induction, the purple urchins are subjected to fattening and spawning induction, and the purple urchins are subjected to spawning induction. According to the method, a multi-recipe combined feeding method adopting baits of the brassica juncea in the fattening period is creatively designed, the design of the GSI index, the egg yolk protein content and the trypsin activity of the sea urchins is combined, the fattening condition and the breeding density in the fattening period are researched and set, and through fattening in the fattening period before spawning induction, the breeding density of the brassica juncea is increased; the method has the advantages that the gonad growth and development of the mature purple urchin in the mature period are obviously improved, the intestinal flora of the purple urchin is effectively improved, the immunity of the purple urchin before spawning induction is enhanced, the spawning rate is also greatly increased, the number and survival rate of fertilized eggs after spawning induction are obviously increased, the artificial reproduction cycle is shortened, and the culture cost is reduced.

The application discloses an egg yolk protein-derived tripeptide with the function of improving osteoporosis and application thereof, and belongs to the technical field of biological medicines. The tripeptide is a tripeptide APW with an amino acid sequence of Ala-Pro-Trp. The peptide segment can be obtained by artificial synthesis or directional enzymolysis of defatted egg yolk powder. Function verification shows that the tripeptide APW and egg yolk protein enzymolysis products containing the peptide segment have the function of improving bone density. In a zebra fish osteoporosis model, when the egg yolk protein enzymolysis product is 20 μg / mL, the bone density is increased by 58%; when the tripeptide APW is 0.5 μg / mL, the bone density is increased by 23.4%; in a rat osteoporosis model, when 10 mg / kg.d of the egg yolk protein enzymolysis product is eaten, the bone density of the femur is increased by 3.39%. Moreover, the food-derived bioactive peptide has high biological safety, so the tripeptide APW and the egg yolk protein enzymolysis product containing the peptide segment can be applied to the preparation of medicines for treating osteoporosis or health care foods for improving bone density, and have good market prospects and application potential.

Methods of targeting a molecule of interest to the oocyte of an animal are shown. In an embodiment the method utilizes a receptor binding region of a yolk protein precursor. In embodiment the yolk protein precursor is a YP1 sequence, functional fragment of functional variant thereof. The targeting molecule is linked to the molecule of interest. The molecule of interest may be a molecule of a gene editing system, such as CRISPR / Cas and in an embodiment comprises Cas. The methods and composition are useful for targeting a molecule of interest to an animal, such as an invertebrate or insect.

Methods of targeting a molecule of interest to the oocyte of an animal are shown. In an embodiment the method utilizes a receptor binding region of a yolk protein precursor. In embodiment the yolk protein precursor is a YP1 sequence, functional fragment of functional variant thereof. The targeting molecule is linked to the molecule of interest. The molecule of interest may be a molecule of a gene editing system, such as CRISPR / Cas and in an embodiment comprises Cas. The methods and composition are useful for targeting a molecule of interest to an animal, such as an invertebrate or insect.

The present application relates to a kind of chilo suppressalis prevention and cure miRNA and its application in cultivating insect-resistant rice.The present application verifies that the overexpression of chilo suppressalis in vivo miRNA-989 significantly reduces the yolk protein deposition in oocyte, causes the number of oocyte in nest to be significantly reduced, further based on amiRNA technology, miR-989 is integrated into rice genome to carry out overexpression, it is verified that the resistance of the rice of the miRNA to chilo suppressalis, and the safety and stability of the rice of the miRNA are evaluated, which provides new germplasm resources for prior art, and provides a new effective prevention and control strategy for green prevention and control of agricultural pests.

The invention discloses a method for non-invasively detecting the estrogen activity of water pollutants by zebrafish, which belongs to the technical field of ecotoxicology, and comprises the following steps: S1, male zebrafish is placed in a 17α-ethynyl estradiol (EE2) standard exposure solution for breeding, and then skin mucus samples are collected to determine the vitellogenin (VTG) concentration; S2, fitting the EE2 exposure concentration-VTG relative concentration standard curve; S3, male zebrafish is placed in a test water sample for breeding, and then mucus samples are collected to determine the VTG concentration; S4, the EE2 equivalent of the water body to be detected is calculated according to the standard curve. The present invention provides a new means for the detection of estrogen activity in water bodies, and the operation steps are simple, and animal dissection is not involved. It only needs to collect fish skin mucus and detect VTG to quickly warn of estrogen analog pollution in water bodies. Compared with the existing detection methods, it has the advantages of high efficiency, accuracy and low cost, and can effectively protect animal welfare.

The invention provides a method for extracting vitelline globulin, which comprises the following steps: freezing yolk liquid for 4-24 hours at the temperature of (-6 DEG C)-(-18 DEG C), then returning the temperature for 12 hours at the temperature of 4 DEG C, adding deionized water for diluting, uniformly stirring under the condition of 8000-12000 rpm, then carrying out solid-liquid separation to obtain lipoprotein and supernate, and filtering the supernate by adopting an ultrafiltration membrane to obtain the vitelline globulin. And filtering the solid, and freeze-drying to obtain the IgY vitelline globulin. The method for extracting the vitelline globulin has the advantages of simplicity and easiness in operation, and is suitable for industrial application.