7 results about "Yolk Proteins" patented technology

The invention belongs to the technical field of biology, and particularly relates to a receptor peptidomimetic capable of improving the breeding efficiency of butter crabs and application of the receptor peptidomimetic. The optimal binding conformation is screened according to the sequence prediction binding conformation of the vitellogen and the receptor of the vitellogen, the hotspot residues are screened according to the optimal binding conformation, and the binding energy change before and after mutation of the hotspot residues is calculated to determine the receptor peptidomimetic sequence. The receptor peptidomimetic can obviously slow down the development speed of the ovary of the blue crab and effectively induce the formation of the butter crab by utilizing the specificity of specific recognition of the vitellogenin of the blue crab and the receptor, so that the butter crab product can be produced in a planned manner.

The application discloses a kind of based on water temperature illumination coupling regulation and control procambarus clarkii ovulation induction system, for the existing system There are many environmental factors, poor coordination ability, temperature monitoring precision is insufficient, lack of real-time perception of physiological state of shrimp problem.The system of the present application includes heat insulation workshop, induced ovulation pool, high-level reservoir, water temperature control module, illumination control module, oxygenation module, water quality monitoring module, microporous camera, dehumidification device, bottom improvement agent automatic feeding device, non-contact yolk protein fluorescence detection probe and intelligent auxiliary system, intelligent auxiliary system is connected with each module respectively Unified control.The present application realizes the time sequence coupling regulation and control of multiple factors such as water temperature, illumination, water level and humidity by coordinating each module through intelligent auxiliary system, and effectively improves the ovulation induction success rate and synchronism of procambarus clarkii by real-time perception of physiological state of shrimp.The present application is mainly used for artificial propagation and seedling scale production of procambarus clarkii.

The invention discloses a method for constructing an anti-spinetoram neoseiulus californicus gene and an ovary targeting peptide of the anti-spinetoram neoseiulus californicus gene. According to the invention, a segment of yolk protein targeted peptide binding region is identified in Neoseiulus californicus, and the yolk protein targeted peptide binding region and a Cas9 sequence are fused and constructed on a PET28a carrier, and the NcvgP2C-Cas9 protein is obtained through prokaryotic expression. NcvgP2C-Cas9 protein, sgNcnAChR alpha 6 and an endosome escape reagent are mixed and injected into female adult mites, NcnAChR alpha 6 gene large fragment deleted neoseiulus californicus offspring is obtained and shows a phenotype with 23.2 times of resistance to spinetoram, and the resistance of a knockout strain to the spinetoram belongs to an autosome partial recessive genetic mode. The invention provides a new method for the genetic improvement of the drug resistance of Neoseiulus californicus, and enhances the compatibility between biological control and pesticide treatment strategies.

The application discloses egg yolk protein osteogenic peptides and a preparation method thereof, and belongs to the fields of biological medicines, functional food or egg product processing technologies. The application adopts defatted egg yolk powder enzymolysis to prepare osteogenic active peptides, and the osteogenic active peptides are enriched and separated through macroporous resins; the preparation and enrichment process is suitable for large-scale production, and can promote transformation from laboratory achievements to industrial production; two osteogenic active peptides are separated and identified, wherein, DFDLPT has the function of promoting osteoblast proliferation activity, and FDIDPG has the function of promoting osteoblast differentiation and mineralization.

The invention belongs to the technical field of biology, and particularly relates to a small molecule peptide for inhibiting development of ovaries of blue crabs and application of the small molecule peptide. According to the method, the optimal binding conformation is screened according to the sequence prediction binding conformation of the vitellogen and the receptor of the vitellogen, the hot residue is screened according to the optimal binding conformation, and the binding energy change before and after mutation of the hot residue is calculated to determine the competitive inhibition polypeptide sequence. The competitive inhibition polypeptide can obviously slow down the development speed of the ovary of the blue crab and effectively induce the formation of the butter crab by utilizing the specificity of specific recognition of the vitellogenin of the blue crab and a receptor, so that the butter crab product can be produced in a planned manner.

The present application relates to a kind of chilo suppressalis prevention and cure miRNA and its application in cultivating insect-resistant rice.The present application verifies that the overexpression of chilo suppressalis in vivo miRNA-989 significantly reduces the yolk protein deposition in oocyte, causes the number of oocyte in nest to be significantly reduced, further based on amiRNA technology, miR-989 is integrated into rice genome to carry out overexpression, it is verified that the resistance of the rice of the miRNA to chilo suppressalis, and the safety and stability of the rice of the miRNA are evaluated, which provides new germplasm resources for prior art, and provides a new effective prevention and control strategy for green prevention and control of agricultural pests.