Fine purification technique for bletilla striata polyoses glue

Abstract

The invention discloses a purification process of Bletilla striata polysaccharide, which belongs to the natural plant polysaccharide deep processing technology field. The process steps are as follows: crude polysaccharide from Bletilla strata is scrubbed with hot alcohol, water adding is performed for redissolution, protease adding is performed for enzymolysis, quenching enzyme and centrifugationare performed, supernatant is collected, ethanol adding is performed for precipitation, the scrubbing is performed with complex solution, and filtration, drying and storage are performed. The products produced through the process have high purity and good dissolution property, and therefore the application range of the products is broadened; the enzyme consumption of the process is small, the rawmaterial is obtained easily, the cost is low, and the industrialization production is feasible.

Description

technical field [0001] The invention belongs to the technical field of deep processing of natural plant polysaccharide gum, and relates to a refining process of bletilla striata polysaccharide gum, in particular to a refining and purification process combining biological enzymatic refining and solvent treatment of bletilla striata polysaccharide gum. Background technique [0002] Bletilla striata [Blettila striata (Thunb.) Reichb.f.] orchidaceae Bletilla striata plant, also known as small bletilla striata, lotus and grass, Xuerumo, etc., is a traditional Chinese medicine resource for both medicine and food, and has been used as a traditional Chinese medicine for thousands of years Its main function is to suppress sores and stop bleeding, nourish the lungs, reduce swelling and promote muscle growth. There are a large amount of aqueous solution-based polysaccharides in the bulbs of Bletilla striata, and its chemical composition is glucomannan, which is the main functional comp...

AI Technical Summary

Problems solved by technology

Although the literature reports and related studies are not very rich, it has brought great inconvenience and difficulty to the purification of Bletilla striata glucomannan, and also provided an opportunity for the necessity of research on the purification technology of Bletilla striata

In practice, some measures such as simple ethanol precipitation, ether and acetone organic solvent washing can significantly improve its purity, but the adsorption operation effect of activated carbon, kaolin and other media is poor

However, the combined solvent washing and precipitation method used alone consumes a lot of organic solvents and costs a lot. Further improvement of purity depends on chromatography technology, enzymatic refining or special chemical treatment methods.

[0004] Bletilla striata bulb polysaccharides have poor senses when used directly. The general extraction process can only produce about 75% of crude polysaccharides. Because it has a typical medicinal smell and darker color, it is limited in food processing and improves the purity of Bletilla striata glucomannan. The above problems can be better solved, but because of the high viscosity of the bletilla striata polysaccharide glue solution, and impurities such as pigments, polyphenols, and proteins are in a bonded state with the polysaccharide, it is difficult to achieve by general refining methods

After the crude bletilla striata polysaccharide is dehydrated and dried, its solubility is reduced, especially when the alcohol precipitation-drying process is used, the problem of instant dissolution is a big defect