Enzyme for producing sea cucumber oligopeptide and enzymolysis technology
A sea cucumber oligopeptide, enzymatic hydrolysis technology, applied in the direction of peptidase, enzyme, pancreatin, etc., can solve the problems that affect the popularization and use, and the taste of the enzymatic hydrolysis solution affects the taste, so as to achieve the product with small molecular weight, short enzymatic hydrolysis time, and product taste Good results
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[0027] Example 1
[0028] Take an appropriate amount of sea cucumber, remove the internal organs, wash, cut into strips, and mince it twice with a meat grinder. It is better if it can be ground into a slurry with a colloid. Weigh 100g of crushed sea cucumber or slurry, add 500ml of purified water, and stir evenly. Add NaHSO to the liquid 3 3mmol / L, heat to 65°C, adjust pH to 9.0 with 10% NaOH, and stir for 20min. Adjust the pH of the treated liquid to 8.2, lower the temperature to 47℃±1℃, add Ca 2+ 3mmol / L, stir well. Add 0.1% compound protease (total protein) to the feed solution and stir for 3 hours. Adjust the pH of the enzymolysis solution to 4.5-4.6, heat up to 65°C-70°C to kill the enzyme, and cool. Add 95% ethanol to the enzymolysis solution until the ethanol concentration of the mixed solution reaches 60%-65% to precipitate polysaccharides. Let it stand for more than 2 hours, centrifuge, and the supernatant is the sea cucumber oligopeptide alcohol solution. After the...
Example Embodiment
[0029] Example 2
[0030] Take an appropriate amount of salted sea cucumber, desalinate it in running water for about 6-8 hours, and take it out. Refining and soaking in purified water for 2-3 times of desalination, 2 hours each time, obtain desalted sea cucumbers. Chop the desalted sea cucumber into small pellets in a chopper, and weigh 10 kg of sea cucumber pellets. The sea cucumber grains are ground into slurry through colloid grinding under pure water running water. Use purified water to adjust sea cucumber grains: pure water = 1:5, and stir evenly. Add Na to the slurry while stirring 2 SO 3 4mmol / L, stir to dissolve evenly. Heat the feed solution to 70°C, adjust the pH to 9.5 with NaOH solution, and stir for 30 minutes. Cool the material liquid to 47°C±1°C, adjust the pH to 8.5±0.1, and add L-cysteine 0.3mmol / L. Add 0.12% compound protease (total protein) to the feed solution, and stir for 3.2 hours. Adjust the pH of the enzymolysis solution to 4.5-4.6, and heat to 7...
Example Embodiment
[0031] Example 3
[0032] Take an appropriate amount of dried sea cucumber and soak it in pure water for more than 24 hours until there are no lumps in the sea cucumber. Take out the soaked sea cucumber and chop it into small pieces in a chopper. Weigh 100 kg of sea cucumber grains. Grind the sea cucumber grains into a slurry through colloid grinding under pure water running water, and put it into the enzymolysis tank. First add the sea cucumber soaking water (water-soluble protein, polysaccharides, etc.), and then use purified water to adjust to the material: water =1:5, stir evenly. Add Na to the slurry while stirring 2 S 2 O 3 5mmol / L, stir to dissolve evenly. Heat the feed solution to 75°C, adjust the pH to 10.0 with NaOH solution, and stir for 40 minutes. Cool the material liquid to 48℃±1℃ and adjust the PH to 8.6±0.1. Join Ca 2+ 0.4mmol / L, L-cysteine salt 0.5mmol / L. Add compound protease 0.15% (total protein) to the feed solution, and stir for 3.5 hours. Adjust the...
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