Method for preparing marine protein peptide through continuous enzymolysis

Abstract

The invention discloses a method for preparing marine protein peptide through continuous enzymolysis. According to the method, sea low value little trash fish is taken as a raw material, after the raw material is ground by a grinding machine, water is added for a homogenate, and homogenate liquid is centrifuged to obtain small trash fish protein fluid; the small trash fish protein fluid is placed into an enzyme membrane reactor to perform an enzymolysis-membrane separation coupled reaction to obtain permeate liquid; the permeate liquid is subjected to concentration and drying treatment to obtain the marine protein peptide powder. According to the method provided by the invention, continuous enzymolysis is performed on substrate protein through the enzyme membrane reactor, and the advantages that enzyme can be repeatedly used, the enzymolysis time is short, the production cost is low, and the quality of finished products is controllable are realized; by adopting animal protein hydrolyzed enzyme, trypsin together for enzymolysis to prepare protein peptide, the disadvantages that single enzyme enzymolysis is insufficient in locus and small in actuating range are overcome, the prepared marine protein peptide is good and heavy in flavor, has no bitter taste, and uniform in product formation. The method provided by the invention can be used for large-scale continuous production, and has good application prospect in the fields of food processing, cosmetics and health care products, medicine and the like.

Description

technical field [0001] The invention relates to the field of deep processing of aquatic products, in particular to a method for preparing marine protein peptides through continuous enzymatic hydrolysis. Background technique [0002] Marine protein peptide is a group of low-molecular-weight oligopeptide mixtures with a relative molecular mass below 1000Da, which are produced by enzymatic hydrolysis of marine fish meat as the main raw material, and can be dissolved by trichloroacetic acid. It is easy to absorb and fast to absorb. , low viscosity, good water solubility and so on. [0003] At present, industrially hydrolyzed protein mostly adopts intermittent production methods. It is a product obtained by inactivating enzymes, desalting, and separating after the protein reaches the desired degree of hydrolysis. The above-mentioned production methods have the disadvantages that enzymes cannot be reused, production costs are high, and products Difficult to control quality, long ...

AI Technical Summary

Problems solved by technology

[0003] At present, industrially hydrolyzed protein mostly adopts intermittent production methods. It is a product obtained by inactivating enzymes, desalting, and separating after the protein reaches the desired degree of hydrolysis. The above-mentioned production methods have the disadvantages that enzymes cannot be reused, production costs are high, and products Disadvantages such as difficult quality control, long reaction time, prone to product inhibition and low production efficiency

Although there are continuous enzymatic hydrolysis methods for preparing protein peptides in the prior art, they all use a single enzyme for enzymatic hydrolysis. Single enzyme enzymatic hydrolysis can only hydrolyze one or a type of protein containing specific amino acid residues, and the scope of action is small. The degree of hydrolysis of the substance is limited

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