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Method for preparing Cordyceps militaris flavochrome powder

A technology for Cordyceps militaris and pigment powder, which is applied to the field of preparation technology of yellow pigment powder, can solve the problems of inability to realize large-scale production, small loading of normal-phase silica gel, difficult recycling and the like, and achieves bright color, short time-consuming, enzyme-free The effect of short solution time

Inactive Publication Date: 2016-10-26
GUANGZHOU AOJIAN PERFUME
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former steps are cumbersome, the yield is not high, and the loading capacity of the normal phase silica gel is small, so it is difficult to recycle.
The latter is expensive and cannot be mass-produced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Dry the fruiting bodies of Cordyceps militaris in an oven at 50°C to constant weight, and pulverize them into powder with a high-speed pulverizer;

[0025] Select chitinase, cellulase and hemicellulase, prepare compound enzyme according to the mass ratio of chitinase, cellulase and hemicellulase at 2:1:1, and then prepare a compound with a mass percentage of 0.2% with water. enzyme solution, and adjust the pH of the composite enzyme solution to 4.0 with 0.1mol / L citric acid solution and 0.2mol / L disodium hydrogen phosphate buffer solution;

[0026] The pretreated Cordyceps militaris powder and the composite enzymatic hydrolysis solution were mixed in a mass ratio of 1:1, and enzymatically hydrolyzed at 30 °C for 30 minutes; the cordyceps militaris-complex enzyme mixture after enzymatic hydrolysis was in a mass ratio of 1:10. Add water, extract at 40 °C for 1 h, filter, take the filtrate, add 80% ethanol, let stand for 24 h, take the supernatant, and remove the ethanol b...

Embodiment 2

[0030] Dry the fruiting bodies of Cordyceps militaris in an oven at 50°C to constant weight, and pulverize them into powder with a high-speed pulverizer;

[0031] Select chitinase, cellulase and hemicellulase, prepare compound enzyme according to the mass ratio of chitinase, cellulase and hemicellulase 3.5:1:1, and then prepare compound enzyme with a mass percentage of 0.4% with water enzyme solution, and adjust the pH of the composite enzyme solution to 5.0 with 0.1mol / L citric acid solution and 0.2mol / L disodium hydrogen phosphate buffer solution;

[0032] The pretreated Cordyceps militaris powder and the compound enzymatic hydrolysis solution were mixed in a mass ratio of 1:3, and enzymatically hydrolyzed at 50 °C for 20 minutes; the cordyceps militaris-complex enzyme mixture after enzymatic hydrolysis was in a mass ratio of 1:15. Add water, extract at 50°C for 2h, filter, take the filtrate, add 70% ethanol, let stand for 24h, take the supernatant, remove the ethanol by rot...

Embodiment 3

[0036] Dry the fruiting bodies of Cordyceps militaris in an oven at 50°C to constant weight, and pulverize them into powder with a high-speed pulverizer;

[0037] Select chitinase, cellulase and hemicellulase, prepare compound enzyme according to the mass ratio of chitinase, cellulase and hemicellulase 5:1:1, and then prepare compound enzyme with a mass percentage of 0.6% with water enzyme solution, and adjust the pH of the composite enzyme solution to 6.0 with 0.1mol / L citric acid solution and 0.2mol / L disodium hydrogen phosphate buffer solution;

[0038] The pretreated Cordyceps militaris powder and the compound enzymatic hydrolysis solution were mixed in a mass ratio of 1:6, and enzymatically hydrolyzed at 60 ° C for 30 minutes; the cordyceps militaris-complex enzyme mixed solution after enzymatic hydrolysis was in a mass ratio of 1:20. Add water, extract at 60 °C for 1 h, filter, take the filtrate, add 80% ethanol, let stand for 24 h, take the supernatant, and remove the e...

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PUM

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Abstract

The invention discloses a method for preparing Cordyceps militaris flavochrome powder. The Cordyceps militaris flavochrome powder is prepared through subjecting Cordyceps militaris, which serves as a raw material, to enzymolysis by a specific compound enzyme, and then, carrying out extracting and drying. According to the method, by the step of carrying out enzymolysis on the Cordyceps militaris through the compound enzyme, the yield of Cordyceps militaris flavochrome can be increased greatly, and the obtained Cordyceps militaris flavochrome is good in stability, has relatively high physiological activity and is high in content of total flavonoids. The method disclosed by the invention is simple in step and easy in operation; and compared with the existing Cordyceps militaris flavochrome extraction methods, the method disclosed by the invention has the advantages of low cost, short time and high yield, thereby being applicable to popularization and application.

Description

technical field [0001] The invention relates to the field of preparation technology of yellow pigment powder, in particular to a method for extracting yellow pigment from Cordyceps militaris and making it into powder. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, belongs to the genus Cordyceps genus of the Ascomycota subphylum Ergot. The orange-yellow color of Cordyceps militaris is caused by its own yellow pigment, which is a carotenoid. Most of the carotenoids discovered so far are fat-soluble carotenoids, which are not easily absorbed by the human body and some are unstable when exposed to light and heat. Cordyceps militaris yellow is a rare class of water-soluble carotenoids discovered so far. [0003] Carotenoids are a large class of pigments in the global ecosystem and are widely distributed in nature. As a precursor of vitamin A, carotenoids help to prevent and cure vitamin A deficiency. At the same time, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00A23L33/10A23K10/30A23K20/105
CPCC09B61/00
Inventor 秦艳康林芝云帆王娜唐惠奇
Owner GUANGZHOU AOJIAN PERFUME
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