Extraction method of chondroitin sulfate

Abstract

The invention belongs to the technical field of biochemicals, and particularly relates to an extraction method of chondroitin sulfate, which comprises the following steps: (1) mixing cartilage and pure water; (2) adding alkaline proteinase, and performing enzymolysis reaction; (3) adding keratinase, and performing enzymolysis reaction; (4) filtering, taking the filtrate, adding an ethanol solution into the filtrate, and settling; and (5) centrifuging the settling solution, taking the precipitate, and drying to obtain the chondroitin sulfate. Compared with salt method extraction and alkaline method extraction, the chondroitin sulfate extraction method provided by the invention simplifies the production process flow; and compared with the current enzyme method extraction mostly adopting trypsinase, the chondroitin sulfate extraction method provided by the invention greatly shortens the process period, improves the extraction yield and simplifies the production process flow.

Description

technical field [0001] The invention belongs to the technical field of biochemical agents, and in particular relates to a method for extracting chondroitin sulfate. Background technique [0002] Chondroitin sulfate is the sulfate ester of chondroitin, an acidic mucopolysaccharide, widely present in the organ cartilage of animals, usually extracted from mammalian cartilage, umbilical cord, blood vessel walls and other tissues, soluble in water, insoluble in Organic solvents such as ethanol and acetone. It can remove lipids and lipoproteins in the blood in the body, and is used to prevent and treat coronary heart disease, angina pectoris, myocardial infarction, atherosclerosis, etc., can effectively promote cell metabolism, and has moderate anticoagulant and anti-inflammatory effects, and accelerate Wound healing and anti-tumor etc. At present, the extraction methods of acidic chondroitin sulfate mainly include degradation method and non-degradation method. The extraction ...

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Problems solved by technology

[0011] The above four kinds of processing routes, the content of chondroitin sulfate and the content of ammonia all meet the requirements of raw materials, but the following defects exist in these four kinds of routes: The sample solution prepared under dilute alkali and mild conditions is turbid and takes a long time. The reason for serious pollution is that the collagen and mucin of cartilage cannot be completely hydrolyzed under this condition, and it is soluble in neutral salt and weak acid solutions. It is not easy to be removed in the solution. The most important thing is that the production cycle of these processes is too long, which greatly limits the production and economic benefits of the factory.

The disadvantage of the above-mentioned method is that concentrated alkali and acid are used in the production process. In this method, the consumption of acid-base alcohol is relatively large, the reaction conditions are relatively strong, and the cost is also high.

[0016] enzymatic hydrolysis-resin extraction method, the advantage of this method is the combination of enzymatic hydrolysis or dilute alkali hydrolysis and resin exchange technology, which ensures that the chondroitin sulfate molecule does not degrade and solves the problem of The problem of the purity of the finished product is solved, the method is simple and the yield is high, but the cost of the method is also high, and it takes a long time

[0017] Judging from the above methods, the method of combining alkaline extraction and trypsin enzymatic hydrolysis is commonly used at home and abroad to extract chondroitin sulfate, but in this extraction process, pancreatic The enzymatic hydrolysis reaction of protease needs at least 5 hours, which is the main rate-limiting link. If this process is used in large-scale production, not only the production cycle is long, but also the product cost is high

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