Preparation method of egg white peptide
An egg white and protease technology, applied in the field of protein polypeptide preparation, can solve the problems of difficult control of the reaction process, large product differences, and large amount of enzyme, and achieve the effects of stable quality performance, low cost, and small amount of enzyme.
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Embodiment 1
[0025] (1) Get 100 kilograms of fresh eggs, shell them, and separate egg whites and egg yolks to obtain 65 kilograms of egg whites;
[0026] (2) The egg white liquid is homogenized by a high-pressure homogenizer, and the homogenization pressure is controlled at 10 ± 2MPa;
[0027] (3) Add pure water with an original volume ratio of 20% to the homogenized egg white liquid, and adjust the pH to 7.5-8.5;
[0028] (4) Heat the egg white liquid to 45±1°C through a plate heat exchanger;
[0029] (5) Through the rotor pump, the pretreated egg white liquid is circulated into the enzyme membrane reactor (Shanghai Ruipai Machinery Co., Ltd. model ZGB-2.5) at a flow rate of 5L / min, and the enzymolysis temperature is controlled by a plate heat exchanger. 40-50°C, check the pH value online, use the pH value adjustment to control the pH value of the reaction process between 7.5-8.5, and cycle the enzymatic hydrolysis for 1 hour;
[0030] (6) The enzymatic solution is continuously sent to ...
Embodiment 2
[0035] (1) Get 100 kilograms of fresh eggs, shell them, and separate egg whites and egg yolks to obtain 65 kilograms of egg whites;
[0036] (2) The egg white liquid is homogenized by a high-pressure homogenizer, and the homogenization pressure is controlled at 10 ± 2MPa;
[0037] (3) Add pure water with an original volume ratio of 20% to the homogenized egg white liquid, and adjust the pH to 6.5-7.5;
[0038] (4) Heat the egg white liquid to 45±1°C through a plate heat exchanger;
[0039] (5) Dissolve 60 grams of dispase with an activity of 20000u / mg in 800 milliliters of water, and slowly add egg white;
[0040] (6) Input the egg white liquid to which the protease has been added into the fermenter, stir with a mixer at a speed of 50 r / min, and react at a temperature of 40-50° C. for 5 hours.
[0041] (7) The enzymatic solution is continuously sent to the ceramic membrane for filtration. The ceramic membrane is selected with a molecular weight of 3000 Daltons and the filtra...
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