Method for extracting tea oil by demulsification based on aqueous enzymatic method

Abstract

The invention discloses a method for extracting tea oil by demulsification based on an aqueous enzymatic method. The method comprises the following steps: processing tea seed powder by microwaves for 60 to 120 seconds; adding distilled water at a material-liquid mass ratio of 1:4 to 1:8, pulping, and collecting upper-layer emulsified liquid; pulping the residual water phase and residues again, and collecting upper-layer emulsified liquid; combining the collected emulsified liquid; adding 0.5-6% of composite enzymatic hydrolysis liquid based on the total mass of the emulsified liquid, performing water bath enzymolysis, centrifuging, and taking upper-layer tea oil; adding an ethanol solution into the residual emulsified liquid, performing water bath treatment, centrifuging, and collecting upper-layer tea oil; combining the collected tea oil, an drying in vacuum. According to the method, the emulsified liquid is high in oil content, and the process is simple, novel and practical; the emulsified liquid is subjected to composite enzyme hydrolysis and ethanol demulsification, so that the enzymatic hydrolysis volume is greatly reduced, the enzyme consumption is greatly reduced, and the process is economic and efficient; microwaves, enzymatic hydrolysis and ethanol are adopted for assisting in oil extraction, so that natural active ingredients of the tea oil are better preserved, and the oil extraction process is safe and nutritive.

Description

technical field

[0001] The invention belongs to the field of grain and oil processing. It relates to an extraction method of camellia oil. Background technique

[0002] The extraction techniques of tea seed oil mainly include traditional pressing method, organic solvent leaching method, Soxhlet extraction method, supercritical extraction method, water substitution method and aqueous enzymatic method, etc. The pressing method and organic solvent leaching method are commonly used in industry.

[0003] The residual oil in the pressing method is relatively high, and the residual oil rate of the cake is as high as 8-12%. The high-temperature treatment destroys the nutritional components, such as the serious denaturation of the protein in the cake, and the tea seed husk mixed in the pressing makes the cake contain a lot of lignin. High, difficult to use; solvent leaching method has safety problems such as large equipment investment and solvent residue in the product; supercritic...

Examples

Embodiment 1

[0020] 1. Camellia oleifera seeds are manually shelled, dried and crushed.

[0021] 2. Weigh 50 g of tea seed powder, microwave for 100 s, add distilled water to extract, the ratio of solid to liquid is 1:5, beat with a colloid mill for 13 min, centrifuge at 4200 r / min for 21 min, repeat 3 times, and collect the emulsion.

[0022] 3. Beat the remaining water phase and residue again with a colloid mill for 9 minutes, let it stand still, and centrifuge at 4200 r / min for 15 minutes, repeat 3 times, and collect the emulsion.

[0023] 4. Combine the emulsions collected twice.

[0024] 5. Add 1% protease, 1.5% phospholipase and 0.5% amylase to the combined emulsion, enzymolyze at 50°C for 3 h, centrifuge, and take the supernatant oil.

[0025] 6. Add 40% ethanol solution with a solid-to-liquid ratio of 1:1 to the remaining emulsion, extract in a water bath for 4 hours, and collect the supernatant oil by centrifugation.

[0026] 7. Combine clean oil and vacuum dry, the oil extracti...

Embodiment 2

[0028] 1. Camellia oleifera seeds are manually shelled, dried and crushed.

[0029] 2. Weigh 1 kg of tea seed powder, microwave for 80 s, add distilled water to extract, the ratio of solid to liquid is 1:4, beat with colloid mill for 12 min, centrifuge at 5000 r / min for 23 min, repeat 3 times, and collect the emulsion.

[0030] 3. Ultrasonicate the remaining water phase and residue for 20 minutes, beat again with a colloid mill for 10 minutes, let stand, centrifuge at 4200 r / min for 12 minutes, repeat 3 times, and collect the emulsion.

[0031] 4. Combine the emulsions collected twice.

[0032] 5. Add 1% protease, 1% phospholipase, 0.5% pectinase and 0.5% amylase to the combined emulsion, enzymatically hydrolyze at 45°C for 2.5 h, centrifuge, and take the supernatant oil.

[0033] 6. Add 45% ethanol solution with a solid-to-liquid ratio of 1:6 to the remaining emulsion, extract in a water bath for 4 hours, and collect the supernatant oil by centrifugation.

[0034] 7. Combin...

Embodiment 3

[0036] 1. Camellia oleifera seeds are manually shelled, dried and crushed.

[0037] 2. Weigh 60 g of tea seed powder, microwave for 70 s, add distilled water to extract, the ratio of solid to liquid is 1:7, beat with colloid mill for 15 min, centrifuge at 4500 r / min for 25 min, repeat 3 times, and collect the emulsion.

[0038] 3. Microwave the remaining water phase and residue for 90 s, beat again with a colloid mill for 8 min, let stand, centrifuge at 4200 r / min for 13 min, repeat 3 times, and collect the emulsion.

[0039] 4. Combine the emulsions collected twice.

[0040] 5. Add 1% protease, 1% phospholipase and 0.5% pectinase to the combined emulsion, hydrolyze at 60°C for 6 h, centrifuge, and take the supernatant oil.

[0041] 6. Add 50% ethanol solution with a solid-to-liquid ratio of 1:3 to the remaining emulsion, extract in a water bath for 4 hours, and collect the supernatant oil by centrifugation.

[0042] 7. Combine clean oil and vacuum dry, the oil extraction ra...

technical field

[0001] The invention belongs to the field of grain and oil processing. It relates to an extraction method of camellia oil. Background technique

[0002] The extraction techniques of tea seed oil mainly include traditional pressing method, organic solvent leaching method, Soxhlet extraction method, supercritical extraction method, water substitution method and aqueous enzymatic method, etc. The pressing method and organic solvent leaching method are commonly used in industry.

[0003] The residual oil in the pressing method is relatively high, and the residual oil rate of the cake is as high as 8-12%. The high-temperature treatment destroys the nutritional components, such as the serious denaturation of the protein in the cake, and the tea seed husk mixed in the pressing makes the cake contain a lot of lignin. High, difficult to use; solvent leaching method has safety problems such as large equipment investment and solvent residue in the product; supercritic...

Examples

Embodiment 1

[0020] 1. Camellia oleifera seeds are manually shelled, dried and crushed.

[0021] 2. Weigh 50 g of tea seed powder, microwave for 100 s, add distilled water to extract, the ratio of solid to liquid is 1:5, beat with a colloid mill for 13 min, centrifuge at 4200 r / min for 21 min, repeat 3 times, and collect the emulsion.

[0022] 3. Beat the remaining water phase and residue again with a colloid mill for 9 minutes, let it stand still, and centrifuge at 4200 r / min for 15 minutes, repeat 3 times, and collect the emulsion.

[0023] 4. Combine the emulsions collected twice.

[0024] 5. Add 1% protease, 1.5% phospholipase and 0.5% amylase to the combined emulsion, enzymolyze at 50°C for 3 h, centrifuge, and take the supernatant oil.

[0025] 6. Add 40% ethanol solution with a solid-to-liquid ratio of 1:1 to the remaining emulsion, extract in a water bath for 4 hours, and collect the supernatant oil by centrifugation.

[0026] 7. Combine clean oil and vacuum dry, the oil extracti...

Embodiment 2

[0028] 1. Camellia oleifera seeds are manually shelled, dried and crushed.

[0029] 2. Weigh 1 kg of tea seed powder, microwave for 80 s, add distilled water to extract, the ratio of solid to liquid is 1:4, beat with colloid mill for 12 min, centrifuge at 5000 r / min for 23 min, repeat 3 times, and collect the emulsion.

[0030] 3. Ultrasonicate the remaining water phase and residue for 20 minutes, beat again with a colloid mill for 10 minutes, let stand, centrifuge at 4200 r / min for 12 minutes, repeat 3 times, and collect the emulsion.

[0031] 4. Combine the emulsions collected twice.

[0032] 5. Add 1% protease, 1% phospholipase, 0.5% pectinase and 0.5% amylase to the combined emulsion, enzymatically hydrolyze at 45°C for 2.5 h, centrifuge, and take the supernatant oil.

[0033] 6. Add 45% ethanol solution with a solid-to-liquid ratio of 1:6 to the remaining emulsion, extract in a water bath for 4 hours, and collect the supernatant oil by centrifugation.

[0034] 7. Combin...

Embodiment 3

[0036] 1. Camellia oleifera seeds are manually shelled, dried and crushed.

[0037] 2. Weigh 60 g of tea seed powder, microwave for 70 s, add distilled water to extract, the ratio of solid to liquid is 1:7, beat with colloid mill for 15 min, centrifuge at 4500 r / min for 25 min, repeat 3 times, and collect the emulsion.

[0038] 3. Microwave the remaining water phase and residue for 90 s, beat again with a colloid mill for 8 min, let stand, centrifuge at 4200 r / min for 13 min, repeat 3 times, and collect the emulsion.

[0039] 4. Combine the emulsions collected twice.

[0040] 5. Add 1% protease, 1% phospholipase and 0.5% pectinase to the combined emulsion, hydrolyze at 60°C for 6 h, centrifuge, and take the supernatant oil.

[0041] 6. Add 50% ethanol solution with a solid-to-liquid ratio of 1:3 to the remaining emulsion, extract in a water bath for 4 hours, and collect the supernatant oil by centrifugation.

[0042] 7. Combine clean oil and vacuum dry, the oil extraction ra...