Method for performing on bagasse by using cellulase-containing multiple enzymes

A technology of cellulase and sugar beet pulp, applied in the direction of fermentation, etc., can solve the problems of large amount of enzyme, low enzymolysis rate, long enzymolysis cycle, etc., and achieve short enzymolysis cycle, high enzymolysis rate and simple equipment Effect

Inactive Publication Date: 2015-06-24
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cellulose is wrapped by hemicellulose and lignin, and the cellulose itself has a tight structure, high crystallinity and polymerization degree, which makes the traditional single cellulose enzymatic hydrolysis use a large amount of enzyme, long enzymatic hydrolysis cycle and low enzymatic hydrolysis rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The multi-enzyme complex is composed of laccase 0.8 U / g, xylanase 5 U / g, and cellulase 10 U / g. The beet pulp is cut into 2 cm pieces, and the multi-enzyme complex and rice straw are put into the enzyme complex. In the solution buffer, the ratio of substrate mass (g) to enzymolysis solution volume (ml) is 1:10, the temperature of the enzymolysis reaction is 45°C, and the enzymolysis reaction is carried out for 48 hours, the glucose content in the enzymolysis solution is measured, and the straw cellulase The solution rate is 58%.

Embodiment 2

[0020] The multi-enzyme complex is composed of laccase 1U / g, pectinase 5 U / g, and cellulase 12U / g. The beet pulp is cut into 2cm pieces, and the multi-enzyme complex and beet pulp are put into enzymatic hydrolysis In the buffer solution, the ratio of substrate mass (g) to enzymolysis solution volume (ml) is 1:15, the temperature of enzymolysis reaction is 50°C, and the enzymolysis reaction is carried out for 72 hours. The solution rate is 62%.

Embodiment 3

[0022] The multi-enzyme complex was composed of 1 U / g of laccase, 0.5 U / g of manganese peroxidase, 3 U / g of xylanase and 10 U / g of cellulase. Put the enzyme complex and corn stalks into the enzymolysis buffer, the ratio of the mass of the substrate (g) to the volume of the enzymolysis solution (ml) is 1:10, the temperature of the enzymolysis reaction is 50°C, and the enzymolysis reaction is carried out for 72 hours, and the enzymolysis is measured Glucose content in the liquid, the enzymatic hydrolysis rate of straw cellulose was 70%.

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Abstract

The invention discloses a method for performing synergic enzymolysis on bagasse by using cellulase-containing multiple enzymes. The substrate enzymolysis is the core link for preparing cellulosic ethanol, and the enhancement of the cellulose enzymolysis rate and the reduction of the cellulase use cost are key factors for implementing cellulosic ethanol industrialization. The multienzyme composite is utilized to perform synergic enzymolysis on the bagasse cellulose. One or combination of several of xylanase, pectinase, laccase, manganese peroxidase and lignin peroxidase and the cellulase constitute the multienzyme composite, and the multienzyme composite is utilized to perform enzymolysis on bagasse under certain conditions. Compared with the traditional cellulose enzymolysis, the method solves the problems of low substrate enzymolysis rate, long enzymolysis cycle and high enzyme consumption in the cellulosic ethanol preparation process, and provides a new method for high-quality utilization of bagasse.

Description

technical field [0001] The invention belongs to the field of biomass enzymatic hydrolysis and saccharification, and in particular relates to a multi-enzyme synergistic enzymatic hydrolysis method for sugar beet pulp comprising cellulase. Background technique [0002] Energy is the basis for the survival and development of human society. The non-renewability of traditional fossil energy, as well as the environmental pollution and greenhouse effect caused by the use, make it unable to meet the needs of human economic and social development. Bioethanol has attracted much attention because of its high octane number and good safety. It can be used as an oxygen-containing additive to replace methyl tert-butyl ether and ethyl tert-butyl ether, and it can also completely replace gasoline as a fuel. After experimental research, many countries have mixed ethanol and gasoline, and the largest ethanol producers are Brazil and the United States. At present, China's existing ethanol pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04
Inventor 李冠华李丹苑琳
Owner INNER MONGOLIA UNIVERSITY
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