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Preparation method of 1,3-dioleoyl-2-palmitoyl triglyceride

A technology of palmitic acid triglyceride and palmitic glyceride, which is applied in 1 field, can solve the problems of long production cycle, large amount of enzyme used, and low energy consumption, and achieve short reaction time, small amount of enzyme used, and low energy consumption Effect

Active Publication Date: 2012-10-31
ZANYU TECH GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a preparation method of 1,3-dioleic acid-2-palmitic acid triglyceride, which has mild process conditions, short reaction time, low energy consumption and low enzyme consumption, and can be prepared with high yield and high yield. The pure 1,3-dioleic acid-2-palmitic acid triglyceride solves the problems of long production cycle, high energy consumption and large amount of enzyme used in the prior art

Method used

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  • Preparation method of 1,3-dioleoyl-2-palmitoyl triglyceride
  • Preparation method of 1,3-dioleoyl-2-palmitoyl triglyceride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 prepares OPO

[0048] (1) Mix 0.1mol of the reaction substrate glycerol and 0.6mol of palmitic acid, add catalyst NaOH (accounting for 0.5% of the total weight of the reaction substrate), and place it in a four-necked round-bottomed flask; place the round-bottomed flask on a constant temperature heating mantle Add a stirring paddle, the stirring speed is 150 rpm, the reaction temperature is 200°C, and the reaction is for 2 hours. During the reaction, nitrogen gas is continuously fed as a protective gas; after the reaction is completed, the free fatty acid in the reaction product is removed with KOH-water alcohol solution;

[0049] Detected by gas phase (FID detector, high-temperature capillary column), the content of tripalmitin in the reaction product is 92.2%, and the content of dipalmitin is 6.6%.

[0050] (2) Take 0.005mol of the reaction product prepared in step (1) (containing 92.2% tripalmitin), 0.03mol of oleic acid (85.8% of oleic acid content) and...

Embodiment 2

[0053] Embodiment 2 prepares OPO

[0054] (1) 1mol of the reaction substrate glycerol and 5mol of palmitic acid are mixed, and the catalyst p-toluenesulfonic acid (accounting for 0.5% of the total weight of the reaction substrate) is added, and placed in a four-necked round-bottomed flask; the round-bottomed flask is placed in a constant temperature heating In the sleeve, add a stirring paddle, the stirring speed is 160 rpm, the reaction temperature is 240°C, and the reaction is for 1 hour. During the reaction, nitrogen gas is continuously fed as a protective gas; after the reaction is completed, the tripalmitin in the product is purified by molecular distillation. ;

[0055] Detected by HPLC (ELSD detector, strong polar ion exchange column), the content of tripalmitin in the product is 98.7%.

[0056] (2) Take 0.05mol of the reaction product prepared in step (1) (containing 98.7% tripalmitin), 0.3mol of oleic acid (95.6% of oleic acid content) and immobilized 1,3-specific li...

Embodiment 3

[0059] Embodiment 3 prepares OPO

[0060] (1) Mix 0.1 mol of the reaction substrate glycerin, 0.8 mol of palmitic acid, Novozym 435 (Novozymes, Denmark) (accounting for 12% of the total weight of the reaction substrate), add in 1L of n-hexane, and react at a temperature of 50°C. 8h; after the reaction is completed, remove free fatty acid with KOH-hydroalcoholic solution;

[0061] Detected by GC (FID detector, high temperature capillary column), the content of tripalmitin in the product is 89.4%.

[0062] (2) get 0.005mol step (1) to prepare the reaction product (containing 89.4% tripalmitin glyceride), 0.03mol oleic acid (oleic acid content 95.6%) and Lpase DF (Japan Amano company) (accounting for reaction substrate 10% of the total weight), reacted in an enzyme reactor at 40° C. for 5 h; centrifuged the reaction mixture, took the supernatant to remove free fatty acids, and added it back to the reactor together with the bottom enzyme. Now measure palmitic acid content 43.2% ...

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Abstract

The invention discloses a preparation method of 1,3-dioleoyl-2-palmitoyl triglyceride, which comprises the following steps: carrying out esterification reaction on glycerol and palmitinic acid under the action of a catalyst to obtain tripalmitin; carrying out ester exchange reaction on the tripalmitin and oleic acid or oleate under the action of 1,3-specific lipase until the ester exchange rate reaches 30-60%, and removing free fatty acids or fatty acid esters in the reaction system; and adding oleic acid or oleate into the reaction system, continuing the reaction until the ester exchange rate reaches 60-90%, and separating and purifying the 1,3-dioleoyl-2-palmitoyl triglyceride from the reaction products. The method disclosed by the invention has the advantages of wide raw material sources, high economy, high safety, mild technological conditions, short reaction time, low energy consumption and low enzyme consumption; and the method can be used for preparing high-yield high-purity 1,3-dioleoyl-2-palmitoyl triglyceride, and can be used as a food nutrient reinforcer for blending infant formula milk powder.

Description

technical field [0001] The invention relates to the field of breast milk fat substitutes, in particular to a preparation method of 1,3-dioleic acid-2-palmitic acid triglyceride. Background technique [0002] 1,3-Dioleoyl-2-palmitic acid triglyceride (1,3-Dioleoyl-2-palmitoyl triglyceride, OPO) is a nutritional enhancer newly approved for use in my country in 2008, mainly used in infant formula, Follow-up infant and young child formula. As we all know, breast milk is the most ideal food for babies, but when normal breast feeding cannot be carried out due to some special reasons, infant formula milk powder is an ideal choice. Milk fat is an important part of breast milk, which only accounts for 3.6%-4.5% in breast milk, but provides 50% of the energy for the baby. About 70% of the palmitic acid in milk fat is located at the Sn-2 position of the triglyceride, while the Sn-1 and Sn-3 positions are mainly oleic acid; however, most of the fats in infant formula milk powder are pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 冯凤琴韦伟陈锡威张辉冯永方
Owner ZANYU TECH GRP CO LTD
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