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Aseptic long-acting enzymolysis method for plant protein

A plant protein and protease technology, applied in the field of plant protein aseptic long-term enzymolysis, can solve problems such as spoilage and deterioration of plant protein, and achieve the effects of prolonging the enzymatic hydrolysis time, reducing the use cost and reducing the risk of contamination.

Inactive Publication Date: 2017-07-21
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of vegetable protein spoilage due to long-term enzymatic hydrolysis in the prior art, the purpose of the present invention is to provide a sterile long-term enzymatic hydrolysis method for vegetable protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Sterile long-term enzymatic hydrolysis of soybean protein isolate

[0030] (1) Weigh 5 g of soybean protein isolate (YP928C beverage-type soybean protein isolate, GB / T20371, 100 mesh, Shandong Yuwang Ecological Food Industry Co., Ltd.) and dissolve it in a 250 mL Erlenmeyer flask filled with 80 mL of distilled water to prepare a feed solution.

[0031] (2) Adjust the pH value of the feed liquid to 7 with 2mol / L sodium hydroxide solution, put it into a sterilized bottle, seal the bottle mouth with a sterilized bottle sealer, and put it in a high-pressure steam sterilizer at 110°C. Sterilize for 20 minutes.

[0032] (3) Put the pipette gun, the sterilized pipette tip, the sterilized filter membrane with a pore size of 0.22 μm (Tianjin Keyilong Experimental Equipment Co., Ltd.), and the sterilized The needle syringe and the cooled feed liquid after sterilization were sterilized by ultraviolet light for 30 minutes; 0.2g neutral protease (Novozymes) and 0.2g flavor protease...

Embodiment 2

[0036] Sterile long-term enzymatic hydrolysis of zein

[0037] (1) Corn gluten (Qinhuangdao Lihua Starch Co., Ltd.) was dry-milled and pulverized with a Joyoung multifunctional cooking machine, and passed through a sieve with a mesh size of 40 or more. The protein content was measured (Kjeldahl method) to be 70%, and it was called Take 10g and add it to a 250mL Erlenmeyer flask, then add 80mL distilled water to dissolve, and prepare a feed liquid.

[0038] (2) Use 2mol / L sodium hydroxide solution to adjust the pH of the feed liquid to 9.0, put it into a sterilized bottle, seal the bottle mouth with a sterilized bottle sealer, and put it into a high-pressure steam sterilizer for sterilization at 121°C Treat for 20min.

[0039] (3) In the aseptic operating table, put the pipette gun, the pipette tip after sterilization, the filter membrane with a pore size of 0.22 μm after sterilization (Tianjin Keyilong Experimental Equipment Co., Ltd.), the sterilized Needle syringe and cool...

Embodiment 3

[0043] Sterile long-acting enzymatic hydrolysis of peanut protein powder

[0044] (1) Weigh 15 g of peanut protein powder with a protein content of 52% (60 mesh, Henan Liangjian Technology Co., Ltd.), add it to a 250 mL conical flask, add 80 mL of water to dissolve and prepare a feed solution.

[0045] (2) Add Na 2 CO 3powder, adjust the pH of the feed solution to 7.0, put it into a sterilized bottle, seal the bottle mouth with a sterilized bottle sealer, and put it into a high-pressure steam sterilizer for sterilization at 121°C for 20 minutes.

[0046] (3) In the aseptic operating table, put the pipette gun, the pipette tip after sterilization, the filter membrane with a pore size of 0.22 μm after sterilization (Tianjin Keyilong Experimental Equipment Co., Ltd.), the sterilized Needle syringe and the cooled feed liquid after sterilization treatment were sterilized by ultraviolet light for 30min; 0.25g of papain (Guangxi Nanning Pangbo Biological Engineering Co., Ltd.) and ...

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Abstract

The invention relates to an aseptic long-acting enzymolysis method for plant protein, and belongs to the technical field of food processing. The method comprises the following steps: preparing ground vegetable protein raw materials or vegetable protein extracts into material liquid by water; making the pH of the material liquid become the optimum pH of protease, and carrying out sterilization; performing aseptic operation,preparingfood product protease into an enzyme solution with required concentration by water, filtering and performing sterilization, adding the filtered and sterilizedenzyme solution to the sterilizedmaterial liquid, thus obtaining a reaction solution; stirring the reaction solution to be fully mixed, and carrying out enzymolysis at the optimum temperature; heating and carrying out enzyme denaturalixation. The method can improve the hydrolysis degree of the vegetable protein, prolong the enzymolysis time, greatly lower theusing amount of enzymes and obviously lower risks of bacterial contamination;the hydrolysis degree of the vegetable protein is more than 18%; the problems of difficultenzymolysis of the vegetable protein, large using amount of the enzymes and easy corruption of enzymolysis liquid during the enzymolysis process are effectively solved; the use cost of the enzymesis lowered; a preservative and an enzyme protection agent are not used, so that components and taste of hydrolysates are not affected, and the application of the hydrolysates is more meaningful.

Description

technical field [0001] The invention relates to a sterile long-acting enzymatic hydrolysis method of vegetable protein, which belongs to the technical field of food processing. Background technique [0002] The peptides and amino acids produced by protein hydrolysis can be widely used in food flavors, dairy products, beverages, health nutrition products, cosmetics, medicine, feed, etc., to provide food nutrients, improve food umami taste, and provide Maillard heat. The precursor of the reaction can enhance the physical properties of food such as emulsification and water holding capacity, exert antioxidant effect, and even some polypeptides have the function of lowering blood pressure. [0003] Common methods of protein hydrolysis mainly include: acid hydrolysis, alkaline hydrolysis and enzymatic hydrolysis. Acid hydrolysis and alkaline hydrolysis can completely hydrolyze protein, but carcinogens such as 1,3-dichloro-2-propanol and 3-chloro-1,2-propanediol will be produced d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/34
CPCA23J3/346
Inventor 周晓宏廖永红林萌莉
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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