Transaminase mutants and application thereof

A transaminase and mutant technology, applied in the field of enzyme engineering, can solve the problems of large column pressure, weak strength, continuous flow reaction, etc., and achieve the effect of increasing possibility, high space-time yield, and reducing emulsification
CN113817699APending Publication Date: 2021-12-21ASYMCHEM LAB TIANJIN
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ASYMCHEM LAB TIANJIN
Publication Date
2021-12-21

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Abstract

The invention provides transaminase mutants and application thereof. The transaminase mutants are subjected to amino acid mutation on the basis of a sequence shown in SEQ ID NO: 1, and the amino acid mutation is single-point mutation or combined mutation of W60Y, Y168A, V379W, V379L, V379M, C418Q and C418W. The activity, stability, temperature tolerance, pH tolerance and organic solvent tolerance of the transaminase mutants are improved, the problem that transaminase is poor in tolerance in an extreme environment in the prior art is solved, and the transaminase mutants are suitable for the field of enzyme engineering.
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Description

technical field

[0001] The invention relates to the field of enzyme engineering, in particular to a transaminase mutant and its application. Background technique

[0002] Chiral amines are important intermediates for the synthesis of many chiral drugs, and are also the main components of optically pure drugs containing amino groups. Neurological drugs, vascular drugs, antihypertensive drugs, anti-infective drugs and vaccines all use chiral amines as intermediates. Transaminases use ketones as raw materials to efficiently produce chiral amines through stereoselective transamination. However, the free enzyme enzymatic method has many problems in the scale-up production application, such as low enzyme activity and large enzyme dosage, which lead to increased fermentation costs; it is easily denatured and inactivated by the influence of organic solvents in the reaction system, and the recovered product after post-treatment is emulsified. , a large amount of three wastes are pr...

Claims

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