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A kind of method using laccase to catalyze the synthesis of silibinin

A technology of silibinin and laccase, which is applied to fermentation and other directions, can solve problems such as long reaction time, and achieve the effects of simple and easy-to-control reaction, simple product separation and purification, and wide temperature range.

Active Publication Date: 2019-01-04
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the synthesis process is still carried out in an organic solvent, and the reaction time is long (96h)

Method used

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  • A kind of method using laccase to catalyze the synthesis of silibinin
  • A kind of method using laccase to catalyze the synthesis of silibinin
  • A kind of method using laccase to catalyze the synthesis of silibinin

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Embodiment 1

[0033] In order to verify the ability of laccase to catalyze the synthesis of silibinin from coniferyl alcohol and docetaxel, the synthesis of silibinin was catalyzed by laccase with coniferyl alcohol and docetaxel as substrates. The production of silibinin was verified when the substrate or enzyme was omitted, as shown in Table 1.

[0034] Accurately weigh a certain amount of coniferyl alcohol and docetaxel, dissolve them in 0.2M PBS (pH6.0) and adjust the volume to 0.555mM and 0.2774mM respectively. Laccase was dissolved in 0.2M PBS (pH 6.0) to 5.335 U / mL. The coniferyl alcohol and docetaxel solutions were stored in the dark at 4°C, and the laccase solution was stored at -20°C, and dissolved on ice before use. The reaction was carried out at 25° C. under the condition of protecting from light, and the reaction time was 1 h. Immediately after the reaction, 100 μL of methanol was added to terminate the enzyme reaction, and placed on ice, protected from light, until sample in...

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Abstract

The invention discloses a method for catalytically synthesizing silybin through laccase, and belongs to the field of biochemical industry. According to the method for catalytically synthesizing the silybin through the laccase, four types of isomerides of the silybin are synthesized by catalyzing coniferyl alcohol and taxifolin through the laccase. The reaction is conducted in a water phase, and use of organic solvent is avoided; the reaction efficiency is high, and 0.9694 mg / L of the silybin can be generated within 1 h. The method for synthesizing the silybin is safe and efficient.

Description

technical field [0001] The invention relates to a method for catalyzing and synthesizing silibinin by using laccase, which belongs to the field of biochemical industry. Background technique [0002] Silymarin is a flavonoid compound extracted from the Compositae plant Silybum marianum Linn.Gaertn. It has a variety of clinical effects and has been included in multinational pharmacopoeias. Silybin (Silybin or Silibinin) is the highest content and most active one of the four isomers of silymarin. It is formed by oxidative coupling of a molecule of Taxifolin and a molecule of Coniferyl alcohol. become. [0003] At present, the main means for people to obtain silybin is to extract it from milk thistle seeds. However, the extraction process is complicated and the cost is high; the growth of milk thistle is affected by seasons and the environment, and it occupies arable land; organic solvents are used in the extraction process, which threatens the safety of the product. In recen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/16
CPCC12P17/162
Inventor 周景文陈坚吕永坤乌仁嘎堵国成丁仁鹏
Owner JIANGNAN UNIV
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