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Process for preparing and refining galactomannan-oligosaccharide by microwave combined enzyme method

A technology of galactomannan oligosaccharides and galactomannan, which is applied in the field of microwave combined enzymatic preparation and refined galactomannan oligosaccharides, can solve the problems of large influence on separation effect, high separation cost, and low separation efficiency , to achieve high preparation efficiency, increase reaction efficiency, and reduce the amount of enzyme used

Inactive Publication Date: 2009-12-02
江苏博立生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvent precipitation method is based on the difference in the solubility of oligosaccharides with different relative molecular weights, and is mainly used to quickly remove high-molecular polysaccharides; column chromatography and resin adsorption methods can separate oligosaccharides more finely, However, the separation effect is greatly affected by the separation medium, but there are disadvantages such as high separation cost and low separation efficiency, so it is generally used for experimental analysis

Method used

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  • Process for preparing and refining galactomannan-oligosaccharide by microwave combined enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] according to figure 1 In the process flow shown, add 10kg of galactomannan gum endosperm slices into the kneading and stirring device, add 0.5kg of sodium hydroxide, 9kg of water, control the temperature at 55°C, and knead and stir for 45 minutes to completely break the film and absorb water. Swell, get alkalized glue.

[0035] Put the alkalized glue in a cabinet-type microwave equipment, adjust the microwave power to 5.0 kilowatts, treat for 40 minutes, and use intermittent turning to prevent the material from coking, and obtain a reduced-viscosity glue.

[0036] After microwave treatment, put the viscosity-reducing glue in a 100L reaction tank, add water and stir, and make a glue solution with a glue concentration of 14.0wt%, adjust the pH to 8.5 with citric acid, and add the enzyme amount according to 5U / g raw film, Add 25.0 g of alkaline β-mannanase with an enzyme activity of 2000 U / g, control the temperature at 55° C., and perform enzymatic hydrolysis for 4.0 hour...

Embodiment 2

[0041] Put 10kg of locust bean galactomannan gum endosperm slices into the kneading and stirring device, add 0.6kg of potassium hydroxide, 8kg of water, control the temperature at 50°C, and knead and stir for 35 minutes to completely break the film and swell with water absorption to obtain alkalized gum .

[0042] Put the alkalized glue in a cabinet-type microwave equipment, adjust the microwave power to 4.0 kilowatts, treat for 40 minutes, and use intermittent turning to keep the material from coking, and obtain a reduced-viscosity glue.

[0043] After microwave treatment, put the viscosity-reducing glue into a 200L reaction tank, add 160L of water, and stir to form a glue solution with a glue concentration of about 5.9wt%. The amount of enzyme added, 25ml of liquid neutral β-mannanase with an enzyme activity of 200U / ml was added, the temperature was controlled at 65°C, and the enzymolysis was carried out for 6.0 hours to obtain an enzymolysis solution.

[0044] The enzymati...

Embodiment 3

[0047] Put 10kg of guar galactomannan gum endosperm slices into the kneading and stirring device, add 0.8kg of potassium hydroxide, 10kg of water, control the temperature at 65°C, knead and stir for 25 minutes, so that the film is completely broken, and the ingredients absorb water and swell to obtain alkalization glue.

[0048] Put the alkalized guar gum in a belt-type microwave equipment, the total microwave input power is 10 kilowatts, adjust the speed so that the microwave treatment time reaches 15 minutes, and monitor the temperature so that the temperature of the material does not exceed 100 ° C to obtain a reduced viscosity .

[0049] After microwave treatment, put the material into a 150L reaction tank, add 90L of water, stir, and make a glue solution with a glue concentration of about 9.0%, adjust the pH to 8.0 with hydrochloric acid, and add the enzyme amount according to 2.5U / g of raw materials, Add 12.5 g of alkaline β-mannanase with an enzyme activity of 2000 U / g...

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Abstract

The invention discloses a process for preparing and refining galactomannan-oligosaccharide by a microwave combined enzyme method, which prepares the galactomannan-oligosaccharide by taking galactomannan glue endosperm slices as raw materials through the steps of kneading alkalization, microwave treatment, enzymolysis, membrane separation refining, and spray drying. The process has the advantages of wide raw material source, short production period, low cost, high oligosaccharide content and the like, and the product can be used as a functional food raw material.

Description

technical field [0001] The invention relates to a process for preparing and refining galactomannan oligosaccharides by microwave combined with enzyme method. Background technique [0002] Oligosaccharides, also known as oligosaccharides, refer to straight-chain or branched-chain sugars formed by connecting 2 to 10 monosaccharides through non-α-1,4-glycosidic bonds. It has good physical and chemical properties such as low heat, stability, safety, non-toxicity, and no residue. It is a recognized functional factor of health food, which can significantly proliferate beneficial bacteria such as bifidobacteria, regulate the balance of intestinal flora; effectively inhibit harmful bacteria and reduce toxin production; enhance immunity, inhibit and prevent tumors; promote digestion and absorption; moisturize Laxative, prevent constipation; promote the absorption of minerals such as calcium, magnesium, iron, zinc, etc. Widely used in dairy products, baby food, health products, medic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/12C12P19/04C12P19/00C07H3/04C07H3/06C07H1/06C08B37/00
Inventor 郭鸿飞许正宏史劲松孙达峰肖光焰黄荣强
Owner 江苏博立生物制品有限公司
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