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Application of 5-aminolevulinic acid in promoting growth of chlorella kjeldahl

A technology of aminolevulinic acid and Pseudochlorella, applied in the direction of microorganism-based methods, adding compounds to stimulate growth, microorganisms, etc., can solve problems such as research without ALA application, achieve promotion of accumulation, simple operation, and raw materials Easy to get effect

Active Publication Date: 2021-08-24
SHANDONG SCIHEALTH BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ALA is mainly used to promote plant growth, but there is no research on the application of ALA in the growth of Pseudochlorella kelvinii

Method used

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  • Application of 5-aminolevulinic acid in promoting growth of chlorella kjeldahl
  • Application of 5-aminolevulinic acid in promoting growth of chlorella kjeldahl
  • Application of 5-aminolevulinic acid in promoting growth of chlorella kjeldahl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Inoculate the cells of Pseudochlorella kaiseri into a glass column equipped with 1000ml f / 2 medium according to the volume fraction of 10%, add 10 mg of 5-aminolevulinic acid to the f / 2 medium, and then carry out aerated culture , cultured in a constant temperature light incubator at 25°C, 3000lux, and cultivated for 16 days; during the cultivation process, 10 mg of 5-aminolevulinic acid was added to the f / 2 medium every 5 days, and Kjeldahl was harvested every 48 hours. For the algae liquid of Chlorella, use the dry weight method to measure the unit biomass of Pseudochlorella kesperi, use the cell counting method to measure the unit biomass of Pseudochlorella kesperi, and use the methanol method to measure the unit biomass of The absorbance value of the algae liquid at 560nm.

Embodiment 2

[0040] The difference from Example 1 is that 30 mg of 5-aminolevulinic acid is added to the f / 2 medium, and the rest of the steps are the same.

Embodiment 3

[0042] The difference from the example is that 30 mg of 5-aminolevulinic acid hydrochloride is added to the f / 2 medium; the culture is carried out at 20° C. in a 5000 lux constant temperature light incubator, and the rest of the steps are the same.

[0043] Control group I

[0044] The difference from Example 1 is that 5-aminolevulinic acid is not added to the f / 2 medium.

[0045] control group II

[0046] The difference from Example 3 is that 5-aminolevulinic acid hydrochloride is not added to the f / 2 medium.

[0047] In Example 1, Example 2 and Control Group I, the results of measuring the absorbance value of the algae liquid of Pseudochlorella kesserii at 560 nm by the methanol method are shown in Table 2.

[0048] Table 2 Absorbance data of each experimental group of 5-aminolevulinic acid at 560nm wavelength

[0049]

[0050]As can be seen from the data in Table 2, the absorbance value measured on the same day, embodiment 1 (the concentration of 5-aminolevulinic acid...

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Abstract

The invention provides application of aminolevulinic acid in promoting growth of pseudochlorella kjeldahl. The application comprises the following specific steps that in the growth process of the pseudochlorella kjeldahl, 5-aminolevulinic acid is added into a culture medium once every 5-8 days, and the 5-aminolevulinic acid can be replaced by a 5-aminolevulinic acid derivative, so that logarithmic phase growth time of the pseudochlorella kjeldahl is prolonged, the increase of the unit biomass of the pseudochlorella kjeldahl is promoted, and the accumulation of photosynthetic pigments of the pseudochlorella kjeldahl is promoted.

Description

technical field [0001] The invention belongs to the technical field of cultivating Pseudochlorella kelvinii, in particular to the application of 5-aminolevulinic acid in promoting the growth of Pseudochlorella kelvinia. Background technique [0002] Pseudochlorella kjeldahl is a kind of green algae. It grows fast and can be cultivated in large quantities without occupying arable land. It is currently widely used in wastewater treatment, bioenergy, and biocarbon sequestration. However, compared with microorganisms such as bacteria, Pseudochlorella kelvinii has a longer culture cycle and relatively slow growth, which has become one of the important constraints affecting time and money costs in the actual production process. How to effectively improve the pseudochlorella kelvinia? The cultivation method of algae can improve the efficiency of photosynthetic growth of algae and shorten its cultivation period, which has important practical significance and application prospects. ...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/38C12N1/12Y02A40/80
Inventor 刘永胜蒲洋吕志成刘兆胜董凯徐晓莹
Owner SHANDONG SCIHEALTH BIOLOGICAL TECH CO LTD
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