Application of NaHS in relieving stress of CuO NPs on tomato seedlings
A tomato seedling and function technology, which is applied in the research field of mitigating plant heavy metal stress technology, can solve the problems of high copper content in tomato seedlings, and achieve the effects of easy acquisition, quality assurance, and improved tolerance
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Embodiment 1
[0016] The effect of NaHS on the growth of tomato seedlings under CuO NPs stress, including the following steps:
[0017] 1) Disinfect tomato seedling seeds with 1% NaClO for 15 minutes, rinse with deionized water for 5-6 times to remove residual NaClO on the surface, and then germinate the seeds evenly in plug trays equipped with vermiculite; When they grow out, they are transferred to the hydroponic solution containing 1 / 4 Hoagland nutrient solution, and the seedlings are placed in an automatic control culture room set at 16 hours of light, 8 hours of darkness, 40% relative humidity, 28°C during the day and 22°C at night Medium culture; Seedlings are cultured twice in 1 / 4 Hoagland nutrient solution and once in 1 / 2 Hoagland nutrient solution; during the day from 7:00 am to 7:00 pm;
[0018] 2) When the tomato seedlings grow to 4 true leaves, select strong seedlings with consistent growth and carry out 6 different treatments, the treatment groups are blank control group (Contr...
Embodiment 2
[0022] To test the effect of NaHS on Cu tissue distribution in tomato seedlings under CuO NPs stress, the steps are as follows:
[0023] 1) Disinfect tomato seedling seeds with 1% NaClO for 15 minutes, rinse with deionized water for 5-6 times to remove residual NaClO on the surface, and then germinate the seeds evenly in plug trays equipped with vermiculite; When they grow out, they are transferred to the hydroponic solution containing 1 / 4 Hoagland nutrient solution, and the seedlings are placed in an automatic control culture room set at 16 hours of light, 8 hours of darkness, 40% relative humidity, 28°C during the day and 22°C at night Medium culture; Seedlings are cultured twice in 1 / 4 Hoagland nutrient solution and once in 1 / 2 Hoagland nutrient solution; during the day from 7:00 am to 7:00 pm;
[0024] 2) When the tomato seedlings grow to 4 true leaves, select strong seedlings with consistent growth and carry out 6 different treatments, the treatment groups are blank contr...
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