A method for detecting photosynthetic pigment content in rape silique peel

A technology for photosynthetic pigments and silique peels, applied in the measurement of color/spectral properties, etc., can solve the problems of poor representation, high cellulose content in silique peels, uneven thickness, etc., to save testing costs, reduce sampling errors, and increase sample volume. Effect

Inactive Publication Date: 2019-07-30
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When using the traditional spectrophotometric method to measure the photosynthetic pigment content of rapeseed silique peel at present, due to the high cellulose content of the silique peel, the difficulty of sample grinding is increased, which is time-consuming and laborious; Harmful to human body, the measurement process causes environmental pollution
In addition, the surface of the rape silique peel is uneven and the thickness is uneven, so when the SPAD method is used to measure the pigment content of the rape silique peel, the error is large and the representativeness is poor.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Select the green silique of rape, pick it off from the stalk, peel it off along the cracking line, remove the grain and diaphragm, flatten it, and cut it into 2.50×0.50cm 2 For a rectangular sample block, accurately measure the area of ​​the outer surface of the silique peel sample with a precision scale, and weigh the weight of the silique peel sample block with a one-thousandth balance;

[0016] (2) scan (1) mesocarp surface area with Yaxin-1241 leaf area meter, obtain scanning value, and convert to I value (unit: scanning value / cm 2 ) and F value (unit: scan value / g); then measure the photosynthetic pigment content C (representing C abx 、C ab and C a 、C b ), the unit is mg / m 2 or mg / g FW; then let C=K i ·I or C=Q i · F, then K i =C / I,Q i =C / F; Finally, determine the coefficient K according to the pigment content C measured by the above spectrophotometric method and the I and F values ​​measured by scanning i , Q i ;

[0017] (3) according to the method...

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PUM

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Abstract

A method for determining the photosynthetic pigment content of oilseed rape pod skin includes the steps of (1), selecting green oilseed rape pods and measuring the superficial area of the pod skin and weighing the pod skin accurately; (2), scanning the superficial area of the pod skin by a Yaxin-1241 leaf area meter and converting the superficial area into a value I and a value F; then, determining the total content Cabx of photosynthetic pigments of the pod skin, the total content Cab of chlorophylls, the content Ca of chlorophylls a and the content Cb of chlorophylls b; setting C=Ki I or C=Qi F, wherein C refers to pigment content, Ki=C / I and Qi=C / F; determining coefficients Ki and Qi according to the determined value C, value I and value F; (3), scanning the superficial area of the oilseed rape pod skin to be determined by the leaf area meter, converting the superficial area into a value I and a value F, and computing the photosynthetic pigment content Cabx, Cab, Ca and Cb of the pod skin to be determined according to the value I, the value F and the coefficients Ki and Qi. The method has the advantages of accurate and reliable results, more samples, fewer sampling errors, convenience in operation, environmental safety and detection cost saving.

Description

technical field [0001] The invention relates to a method for detecting the content of photosynthetic pigment, in particular to a method for detecting the content of photosynthetic pigment in the silique peel of rapeseed. Background technique [0002] Photosynthetic pigments participate in the absorption and transmission of light energy and cause the original photochemical reaction in plant photosynthesis. There are three main categories of photosynthetic pigments in higher plants: chlorophyll a, b and carotenoids. Chlorophyll has maximum absorption in the red and blue light regions, and carotenoids have maximum absorption in the blue light region. The maximum absorption peaks of chlorophyll a and b are around 663nm and 645nm in the red light region, respectively, and around 429nm and 453nm in the blue-violet light region. [0003] According to the light absorption of photosynthetic pigments, the determination methods of plant photosynthetic pigment content mainly include u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 王春丽刘景玲梁宗锁陈洁杨建利王周礼
Owner NORTHWEST A & F UNIV
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