Method for improving baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate
By treating tobacco leaves with exogenous methyl jasmonate, the activity of their antioxidant enzymes is enhanced, which solves the problems of oxidative damage and browning during the tobacco leaf baking process, achieves efficient antioxidant capacity improvement and quality maintenance of tobacco leaves, and is suitable for large-scale promotion.
Patent Information
- Application Number
- CN202510754299.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-06
- Publication Date
- 2025-10-03
AI Technical Summary
During the tobacco leaf baking process, due to oxidation and enzymatic browning, tobacco leaves are prone to problems such as dull color, accelerated browning, and quality degradation, which affects their commercial value. Existing technologies are difficult to accurately control and the breeding cycle is long.
Exogenous methyl jasmonate was used to treat tobacco leaves. By spraying methyl jasmonate solution, the antioxidant enzyme activity of tobacco leaves was enhanced, the accumulation of reactive oxygen species was reduced, and the oxidative damage of cell membranes was delayed. The tobacco leaves were sprayed immediately from the maturity period to the harvest to ensure that the leaf surface was completely covered. The leaves were moved into a closed dark box to observe the browning state. The antioxidant capacity improvement coefficient was calculated by combining the activities of multiple antioxidant enzymes and the total antioxidant capacity.
It can effectively reduce oxidative damage to tobacco leaves, delay browning, enhance the antioxidant capacity of tobacco leaves, maintain cell membrane integrity, and reduce quality degradation without changing the existing planting and baking processes. It is suitable for large-scale promotion and meets green production requirements.
Smart Images
Figure CN120732182A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of tobacco leaf baking, and specifically relates to a method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate. Background Art
[0002] Tobacco leaf curing is a crucial step in the tobacco production process, directly impacting its chemical composition, aroma quality, and ultimate industrial viability. During the traditional curing process, oxidation and enzymatic browning often cause tobacco leaves to lose color, accelerate browning, and deteriorate in quality, severely impacting their commercial value. Currently, the industry primarily mitigates this browning problem by adjusting curing temperature and humidity or through selective breeding. However, traditional temperature and humidity control methods are difficult to precisely control, require long breeding cycles, and are slow to adopt, making them inadequate for market demand.
[0003] In recent years, the role of plant hormones in the regulation of crop growth has received widespread attention. Among them, jasmonic acid (JA), as an important plant hormone, plays an important role in plant stress resistance, secondary metabolism regulation and growth and development. Studies have shown that jasmonic acid and its derivatives can enhance the tolerance of plants to abiotic stresses (such as drought and high temperature) and affect the formation of cell walls and the stability of cell membranes. However, in the field of tobacco leaf curing, there are few studies on how exogenous jasmonic acid can improve the curing resistance of tobacco leaves, and existing technical means still have certain limitations. Therefore, exploring methods for treating tobacco leaves with exogenous jasmonic acid to improve their curing resistance can not only reduce quality loss during the curing process, but also improve the industrial availability of tobacco leaves, which has important theoretical value and application prospects. The present invention aims to provide a method for enhancing the curing resistance of tobacco leaves using exogenous jasmonic acid, so as to improve the chemical stability and physical structure of tobacco leaves during the curing process, thereby improving the comprehensive quality and adaptability of tobacco leaves.
[0004] Therefore, those skilled in the art have proposed a method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate to solve the problems raised in the background art. Summary of the Invention
[0005] In order to solve the above technical problems, the present invention provides a method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate, so as to solve the problem that in the baking process of the prior art, tobacco leaves often suffer from dull color, accelerated browning, and quality degradation due to oxidation and enzymatic browning, which seriously affects their commercial value.
[0006] A method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate comprises the following steps: S1: diluting the methyl jasmonate to a specified concentration, and after the methyl jasmonate mother solution is prepared, storing it in a sealed and light-proof condition at a low temperature;
[0007] S2: Harvesting the tobacco leaves when their color changes from dark green to yellow-green, their main veins become brighter, and their leaves show obvious mature spots;
[0008] S3: spraying the exogenous methyl jasmonate solution from the tobacco leaf maturity stage to immediately after harvest, ensuring that the tobacco leaf surface is completely covered with mist droplets and does not flow down in streams;
[0009] S4: moving the tobacco leaves into a dark, airtight box, observing the browning state of the tobacco leaves at specified intervals, and calibrating the baking resistance grade of the tobacco leaves with reference to standards;
[0010] S5: The content of membrane lipid peroxides and malondialdehyde was determined by thiobarbituric acid colorimetry; the content of superoxide anions was determined by pyrogallol method; the content of hydrogen peroxide was determined by resorcinol colorimetry; the activity of antioxidant enzymes and total antioxidant capacity were determined by a special kit for microenzyme activity;
[0011] S6: Calculating the tobacco leaf antioxidant capacity improvement coefficient using formula (1) based on the measurement results of the antioxidant enzyme activity and the total antioxidant capacity;
[0012]
[0013] Wherein, K is the antioxidant capacity improvement coefficient of the tobacco leaf, A 处理后 is the antioxidant capacity of the tobacco leaves after spraying the exogenous methyl jasmonate solution, A 处理前 It is the antioxidant capacity of the tobacco leaves before spraying the exogenous methyl jasmonate solution.
[0014] Preferably, in the step of preparing the methyl jasmonate mother liquor, the mass fraction of the methyl jasmonate is 98%, and the concentration of the methyl jasmonate mother liquor is 100 μmol / L.
[0015] Preferably, in the step of spraying the exogenous methyl jasmonate solution, the spraying concentration of the exogenous methyl jasmonate solution is 50-200 μmol / L, and the calculation formula of the spraying concentration is:
[0016] Wherein C is the spraying concentration, C max and C min are the upper and lower limits of the spraying concentration, M is the maturity index of the tobacco leaves, and M max and M min are the upper and lower limits of the maturity index respectively.
[0017] Preferably, in the step of identifying the baking characteristics, the tobacco leaves are moved into a sealed, airtight, dark box, and the front and back of the tobacco leaves are photographed at a fixed position and under fixed light using a digital camera every 12 hours to record the color changes of the tobacco leaves.
[0018] Preferably, in the step of detecting the antioxidant level of the tobacco leaves, the antioxidant enzyme activity includes superoxide dismutase activity, catalase activity and glutathione reductase activity, and the total antioxidant capacity is determined by FRAP method.
[0019] Preferably, in the step of calculating the tobacco leaf antioxidant capacity improvement coefficient, the antioxidant capacity includes the antioxidant enzyme activity and the total antioxidant capacity.
[0020] Preferably, in the step of preparing the methyl jasmonate mother liquor, the low-temperature sealed light-proof condition is sealed and light-proof storage at 4°C.
[0021] Preferably, in the step of spraying the exogenous methyl jasmonate solution, the time interval between spraying the exogenous methyl jasmonate solution from the maturity stage of the tobacco leaves to immediately after harvesting does not exceed 30 minutes.
[0022] Preferably, the photographs taken are analyzed by image analysis software to calculate the percentage of the browning area of the tobacco leaves to the total leaf area. The calculation formula for the browning area is: Among them, P 褐变 is the percentage of the browning area of the tobacco leaf, A 褐变 is the area of the brown part of the tobacco leaf, A 总 is the total leaf area of the tobacco leaves.
[0023] Preferably, in the step of detecting the antioxidant degree of the tobacco leaves, the determination results of the antioxidant enzyme activity and the total antioxidant capacity are used to evaluate the improvement of the antioxidant capacity of the tobacco leaves. The evaluation formula for the improvement of the antioxidant capacity is: Wherein E is the improvement of the antioxidant capacity, I 处理后 -I 处理前 They are the comprehensive index of antioxidant capacity of tobacco leaves before and after spraying exogenous methyl jasmonate solution.
[0024] Through the above technical solution,
[0025] Compared with the prior art, the present invention has the following beneficial effects:
[0026] 1. The methyl jasmonate used in the present invention can enhance the activity of antioxidant enzymes in tobacco leaves (such as superoxide dismutase SOD, catalase CAT, and peroxidase POD), reduce the accumulation of reactive oxygen species (ROS), reduce oxidative damage from the root, maintain cell membrane integrity by reducing membrane lipid peroxidation, slow down water loss, and thus delay the browning of tobacco leaves.
[0027] 2. The present invention emphasizes spraying methyl jasmonate immediately after tobacco leaves are harvested, which can delay cell senescence and reduce quality degradation caused by over-maturity or delayed harvesting.
[0028] 3. The present invention only requires spraying an appropriate amount of methyl jasmonate solution after the tobacco leaves are harvested. This does not change the existing planting, harvesting, and curing processes, and does not require the addition of additional chemical antioxidants. This method is simple and easy to implement, making it suitable for large-scale promotion. Methyl jasmonate, as a natural plant signaling molecule, can be degraded in plants, eliminating the problem of pesticide residues, meeting the requirements of green tobacco production and being environmentally safe. BRIEF DESCRIPTION OF THE DRAWINGS
[0029] Figure 1 It is a schematic diagram of the overall structure of the present invention;
[0030] Figure 2 This is a graph showing changes in membrane lipid oxidation metabolism indicators in the present invention;
[0031] Figure 3 This is a graph showing changes in the activity of antioxidant-related enzymes in the present invention. DETAILED DESCRIPTION
[0032] The following embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples. The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.
[0033] Example 1: As shown in the attached Figure 1-3 The present invention provides a method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate, comprising the following steps: S1: diluting the methyl jasmonate to a specified concentration;
[0034] After the methyl jasmonate mother solution is prepared, it is stored in a sealed and light-proof environment at a low temperature;
[0035] S2: Harvesting the tobacco leaves when their color changes from dark green to yellow-green, their main veins become brighter, and their leaves show obvious mature spots;
[0036] S3: spraying the exogenous methyl jasmonate solution from the tobacco leaf maturity stage to immediately after harvest, ensuring that the tobacco leaf surface is completely covered with mist droplets and does not flow down in streams;
[0037] S4: moving the tobacco leaves into a dark, airtight box, observing the browning state of the tobacco leaves at specified intervals, and calibrating the baking resistance grade of the tobacco leaves with reference to standards;
[0038] S5: The content of membrane lipid peroxides and malondialdehyde was determined by thiobarbituric acid colorimetry; the content of superoxide anions was determined by pyrogallol method; the content of hydrogen peroxide was determined by resorcinol colorimetry; the activity of antioxidant enzymes and total antioxidant capacity were determined by a special kit for microenzyme activity;
[0039] S6: Calculating the tobacco leaf antioxidant capacity improvement coefficient using formula (1) based on the measurement results of the antioxidant enzyme activity and the total antioxidant capacity;
[0040]
[0041] Wherein, K is the antioxidant capacity improvement coefficient of the tobacco leaf, A 处理后 is the antioxidant capacity of the tobacco leaves after spraying the exogenous methyl jasmonate solution, A 处理前 It is the antioxidant capacity of the tobacco leaves before spraying the exogenous methyl jasmonate solution.
[0042] Preferably, in the step of preparing the methyl jasmonate mother liquor, the mass fraction of the methyl jasmonate is 98%, and the concentration of the methyl jasmonate mother liquor is 100 μmol / L. The preparation method and concentration of the mother liquor are clarified, and the purity and stability of the mother liquor are ensured. Through precise preparation of the mother liquor, the uniformity and effectiveness of the exogenous methyl jasmonate are guaranteed, and the influence of impurities and microorganisms on the tobacco leaves is avoided.
[0043] Figure 2 It is the change of membrane lipid oxidation metabolism index, Figure 3 The changes in antioxidant-related enzyme activities, CK is the control group, MeJA is the methyl jasmonate treatment group
[0044] As can be seen from the above, when using, 1.06 mL of 98% by mass methyl jasmonate is diluted to 10 mL. After the methyl jasmonate mother liquor is prepared, it needs to be sealed and stored away from light at 4°C. When the color of the tobacco leaves changes from dark green to yellow-green, the main veins become brighter, and the leaves show obvious mature spots, they are harvested. From the maturity period of the tobacco leaves to the harvest, an exogenous methyl jasmonate solution is sprayed immediately at a concentration of 50-200 μmol / L. The leaves are ensured to be completely covered with mist droplets and not to flow down in streams. The tobacco leaves are moved into a dark, airtight box. The browning state of the tobacco leaves is observed every 12 hours. Referring to the standard "YC / T311-2009", the baking resistance grade of each sample is calibrated by recording the time when the tobacco leaves turn 30% brown. The contents of membrane lipid peroxide (LPO) and malondialdehyde (MDA) are determined by the thiobarbituric acid (TBA) colorimetric method; the superoxide anion (O2-) is determined by the pyrogallol method; and the hydrogen peroxide (H2O2) content is determined by the resorcinol colorimetric method. Antioxidant enzyme activities and total antioxidant capacity were determined using microenzyme activity kits. Superoxide dismutase (SOD) activity was determined using the NBT method; catalase (CAT) and glutathione reductase (GR) activities were determined using UV absorption spectrometry; and total antioxidant capacity was determined using the FRAP method.
[0045] Example 2: As shown in the attached Figure 1 As shown: This embodiment is basically the same as the previous embodiment, except that, in the step of spraying the exogenous methyl jasmonate solution, the spraying concentration of the exogenous methyl jasmonate solution is 50-200 μmol / L, and the calculation formula of the spraying concentration is:
[0046] Wherein C is the spraying concentration, C max and C min are the upper and lower limits of the spraying concentration, M is the maturity index of the tobacco leaves, and M max and M min They are the upper and lower limits of the maturity index respectively. The spraying concentration is dynamically adjusted according to the variety and maturity of the tobacco leaves, which improves the accuracy of the treatment. By dynamically adjusting the spraying concentration, it can better adapt to the needs of tobacco leaves of different maturity, improve the treatment effect, ensure that the spraying concentration of exogenous methyl jasmonate matches the actual needs of the tobacco leaves, and further improve the baking resistance of the tobacco leaves.
[0047] Specifically, in the step of identifying the baking characteristics, the tobacco leaves are moved into a sealed, airtight, dark box, and photographed from the front and back of the tobacco leaves at a fixed position and under fixed light every 12 hours using a digital camera to record the color changes of the tobacco leaves. The photographs are analyzed using image analysis software to calculate the percentage of the browning area of the tobacco leaves to the total leaf area. The calculation formula for the browning area is: Among them, P 褐变 is the percentage of the browning area of the tobacco leaf, A 褐变 is the area of the brown part of the tobacco leaf, A 总 The total leaf area of the tobacco leaves is used to quantify the browning degree of the tobacco leaves through image analysis technology, thereby improving the accuracy of the baking resistance assessment. Compared with manual observation, the baking resistance of the tobacco leaves can be assessed more accurately, avoiding the subjectivity of human judgment.
[0048] Furthermore, in the step of detecting the antioxidant degree of the tobacco leaves, the antioxidant enzyme activity includes superoxide dismutase activity, catalase activity and glutathione reductase activity, and the total antioxidant capacity is determined by the FRAP method. By comprehensively evaluating the antioxidant enzyme activity and the total antioxidant capacity, the antioxidant performance of the tobacco leaves can be more comprehensively reflected. In the step of calculating the antioxidant capacity improvement coefficient of the tobacco leaves, the antioxidant capacity includes the antioxidant enzyme activity and the total antioxidant capacity.
[0049] As can be seen from the above, this method provides detailed regulations for adjusting the spray concentration of the exogenous methyl jasmonate solution, observing methods for identifying curing characteristics, and determining the indicators and methods for antioxidant capacity testing. By dynamically adjusting the spray concentration, the amount of exogenous methyl jasmonate can be precisely controlled according to the maturity of the tobacco leaves, ensuring optimal treatment results for tobacco leaves of varying maturity, thereby improving their curing resistance. Specific observation methods for identifying curing characteristics are clarified, and by regularly photographing tobacco leaves and recording color changes, intuitive and accurate data support is provided for curing resistance assessment. Specific indicators and methods for antioxidant capacity testing are also specified, including the determination of multiple antioxidant enzyme activities and total antioxidant capacity. This allows for a comprehensive assessment of the antioxidant properties of tobacco leaves, providing a scientific basis for improving curing resistance.
[0050] Example 3: As shown in the attached Figure 1 As shown in Example 1, in the step of preparing the methyl jasmonate mother liquor, the low-temperature sealed light-proof condition is 4° C. sealed light-proof storage. This step clarifies the storage conditions of the mother liquor and ensures the stability of the mother liquor. Through strict storage conditions, the stability and effectiveness of the mother liquor are ensured, and degradation of the mother liquor during storage is avoided.
[0051] Specifically, in the step of spraying the exogenous methyl jasmonate solution, the time interval between spraying the exogenous methyl jasmonate solution from the maturity stage of the tobacco leaves to immediately after harvesting does not exceed 30 minutes. By strictly controlling the spraying time interval, the antioxidant effect of the exogenous methyl jasmonate can be better exerted, and the baking resistance of the tobacco leaves can be improved.
[0052] Furthermore, in the step of detecting the antioxidant degree of the tobacco leaves, the determination results of the antioxidant enzyme activity and the total antioxidant capacity are used to evaluate the improvement of the antioxidant capacity of the tobacco leaves. The evaluation formula for the improvement of the antioxidant capacity is: Wherein E is the improvement of the antioxidant capacity, I 处理后 -I 处理前 These are the comprehensive antioxidant capacity indexes of tobacco leaves before and after spraying with exogenous methyl jasmonate solution. By calculating the improvement in antioxidant capacity, the effect of exogenous methyl jasmonate on the antioxidant capacity of tobacco leaves can be more intuitively evaluated.
[0053] As can be seen from the above, this method has made detailed provisions for the storage conditions of methyl jasmonate mother liquor, the time interval for spraying exogenous methyl jasmonate solution, the image analysis method in the identification of baking characteristics, and the evaluation of the improvement of antioxidant capacity. It clarifies that the storage conditions of the mother liquor are sealed and away from light at 4°C to ensure the stability and effectiveness of the mother liquor and avoid degradation during storage. At the same time, it is stipulated that the time interval for spraying exogenous methyl jasmonate solution shall not exceed 30 minutes to ensure that the exogenous methyl jasmonate can take effect in time and improve the baking resistance of tobacco leaves. By calculating the improvement of antioxidant capacity, the treatment effect of exogenous methyl jasmonate is quantified, which provides an intuitive indicator for evaluating the improvement of the antioxidant capacity of tobacco leaves, ensures the scientificity and reliability of exogenous methyl jasmonate treatment of tobacco leaves, and provides comprehensive technical support for improving the quality and baking resistance of tobacco leaves.
[0054] The embodiments of the present invention are given for the purpose of illustration and description. Although the embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Those skilled in the art can change, modify, replace and modify the above embodiments within the scope of the present invention. In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. The meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.
[0055] In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "connect," "fixed," etc. should be understood broadly. For example, they may refer to fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.
[0056] In the present invention, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediary. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.
[0057] In the description of this specification, the reference terms "one embodiment", "some embodiments", "examples", "specific examples" or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.
[0058] In the drawings of the embodiments disclosed in the present invention, only the structures involved in the embodiments disclosed in the present invention are involved. Other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.
[0059] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate, characterized in that: The method comprises the following steps: S1: diluting methyl jasmonate to a specified concentration, and storing the methyl jasmonate mother solution at a low temperature, sealed and light-proof condition after the preparation is completed; S2: Harvesting the tobacco leaves when their color changes from dark green to yellow-green, their main veins become brighter, and their leaves show obvious mature spots; S3: spraying the exogenous methyl jasmonate solution from the tobacco leaf maturity stage to immediately after harvest, ensuring that the tobacco leaf surface is completely covered with mist droplets and does not flow down in streams; S4: moving the tobacco leaves into a dark, airtight box, observing the browning state of the tobacco leaves at specified intervals, and calibrating the baking resistance grade of the tobacco leaves with reference to standards; S5: The content of membrane lipid peroxides and malondialdehyde was determined by thiobarbituric acid colorimetry; the content of superoxide anions was determined by pyrogallol method; the content of hydrogen peroxide was determined by resorcinol colorimetry; the activity of antioxidant enzymes and total antioxidant capacity were determined by a special kit for microenzyme activity; S6: Calculating the tobacco leaf antioxidant capacity improvement coefficient using formula (1) based on the measurement results of the antioxidant enzyme activity and the total antioxidant capacity; Wherein, K is the antioxidant capacity improvement coefficient of the tobacco leaf, A 处理后 is the antioxidant capacity of the tobacco leaves after spraying the exogenous methyl jasmonate solution, A 处理前 It is the antioxidant capacity of the tobacco leaves before spraying the exogenous methyl jasmonate solution.
2. The method of claim 1 , wherein: In the step of preparing the methyl jasmonate mother liquor, the mass fraction of the methyl jasmonate is 98%, and the concentration of the methyl jasmonate mother liquor is 100 μmol / L.
3. The method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate as claimed in claim 1, characterized in that: In the step of spraying the exogenous methyl jasmonate solution, the spraying concentration of the exogenous methyl jasmonate solution is 50-200 μmol / L, and the calculation formula of the spraying concentration is: Wherein C is the spraying concentration, C max and C min are the upper and lower limits of the spraying concentration, M is the maturity index of the tobacco leaves, and M max and M min are the upper and lower limits of the maturity index respectively.
4. The method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate as claimed in claim 1, characterized in that: In the step of identifying the baking characteristics, the tobacco leaves are moved into a sealed, airtight, dark box, and the front and back of the tobacco leaves are photographed at a fixed position and under fixed light using a digital camera every 12 hours to record the color changes of the tobacco leaves.
5. The method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate as claimed in claim 1, characterized in that: In the step of detecting the antioxidant degree of the tobacco leaves, the antioxidant enzyme activity includes superoxide dismutase activity, catalase activity and glutathione reductase activity, and the total antioxidant capacity is determined by FRAP method.
6. A method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate as claimed in claim 5, characterized in that: In the step of calculating the tobacco leaf antioxidant capacity improvement coefficient, the antioxidant capacity includes the antioxidant enzyme activity and the total antioxidant capacity.
7. The method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate as claimed in claim 3, characterized in that: In the step of preparing the methyl jasmonate mother solution, the low-temperature sealed light-proof condition is sealed and light-proof storage at 4°C.
8. The method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate as claimed in claim 1, characterized in that: In the step of spraying the exogenous methyl jasmonate solution, the time interval between spraying the exogenous methyl jasmonate solution from the tobacco leaf maturity stage to immediately after harvesting is no more than 30 minutes.
9. The method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate as claimed in claim 4, characterized in that: The photographs were analyzed using image analysis software to calculate the percentage of the browning area of the tobacco leaves to the total leaf area. The calculation formula for the browning area is: Among them, P 褐变 is the percentage of the browning area of the tobacco leaf, A 褐变 is the area of the brown part of the tobacco leaf, A 总 is the total leaf area of the tobacco leaves.
10. The method for improving the baking resistance of tobacco leaves by utilizing exogenous methyl jasmonate as claimed in claim 6, characterized in that: In the step of detecting the antioxidant degree of the tobacco leaves, the determination results of the antioxidant enzyme activity and the total antioxidant capacity are used to evaluate the improvement of the antioxidant capacity of the tobacco leaves. The evaluation formula for the improvement of the antioxidant capacity is: Wherein E is the improvement of the antioxidant capacity, I 处理后 -I 处理前 They are the comprehensive index of antioxidant capacity of tobacco leaves before and after spraying exogenous methyl jasmonate solution.