Method for creating easily-industrialized breeding Cymbidium sinense resources

It is a factory-like technology of Molan, applied in the field of plant germplasm resource innovation, which can solve the problems of reduced efficiency, incompatibility with the requirements of the promotion of new varieties of Molan and industrialization development, and weakened competitiveness, so as to save manpower and material resources, improve The effect of tissue culture rapid propagation ability and shortening breeding years

Active Publication Date: 2018-02-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is difficult to reproduce the traditional molan seedlings in factories, and it is impossible to realize the factory production of seedlings
Therefore, the traditional molan is still propagated by ramets, which is slow and does not meet the requirements of the promotion of new varieties and industrial development of molan. weakened capacity
In recent years, due to the long-term division propagation, the quality of molan seedlings has declined, the disease resistance has decreased, and stem rot has frequently occurred, which has caused huge economic losses to molan producers. Some molan producers have to give up molan production

Method used

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  • Method for creating easily-industrialized breeding Cymbidium sinense resources
  • Method for creating easily-industrialized breeding Cymbidium sinense resources
  • Method for creating easily-industrialized breeding Cymbidium sinense resources

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: the establishment of easy factory breeding molan resources

[0051] Since 2011, we have used the method described in the present invention to carry out the creation of Molan resources that are easy to reproduce in factories. The specific method is as follows (flow process is as follows: figure 1 shown):

[0052] S1. Parent selection and hybrid combination configuration: the hybrid orchid 'Yu Nilan' obtained by crossing 'Qijianbaimo' Molan and 'Jinjiao' Cymbidium, which contains half of the Molan genome, was selected as the core parent material, in 2011 On February 20, a hybrid combination was prepared with 'Yonuilan' as the female parent and 'Huangyehongmo' Molan as the male parent, and the fruit was harvested on August 24 of the same year.

[0053] S2. Single-seed culture and intermediate propagule morphology and proliferation ability identification:

[0054] S21. Fruit disinfection, inoculation and primary culture: use a scalpel to remove the fruit sta...

Embodiment 2

[0075] Example 2: Tissue culture rapid propagation characteristics of d26 and d521

[0076] In order to further verify whether the obtained new resources are easier to carry out industrial reproduction than the traditional Molan, the traditional Molan 'Qijian Baimo' was used as a control, and the tissue culture experiment was carried out together with d26 and d521.

[0077] 1. Proliferation characteristics of rhizomes

[0078] Proliferation experiments were carried out using uniformly grown rhizomes of 'Qijianbaimo' Molan, d26 and d521, only the concentration of 6-BA in the medium was changed (1.0mg / L, 1.5mg / L and 2.0mg / L) , the rest of the medium components and culture conditions were kept the same, weighed before culturing and after 40 days of culturing, and calculated the absolute proliferation rate (absolute proliferation rate (%)=(gross weight after culturing-initial gross weight) / initial gross weight×100%) . The test results are shown in Table 4. The absolute prolifera...

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Abstract

The invention discloses a method for creating easily-industrialized breeding Cymbidium sinense resources, belonging to the field of innovation of plant germplasm resources. The method comprises the following steps: selecting hybrid Cymbidium containing a Cymbidium sinense genome as core parents so as to prepare a hybrid combination, carrying out single-seed in-vitro culturing and evaluating the type and the tissue culturing and rapid propagation abilities of an intermediate propagule of hybrid generations, producing test-tube seedlings, identifying produced test-tube seedlings, and carrying out transplanting so as to obtain the easily-industrialized breeding Cymbidium sinense resources. Compared with a conventional breeding method which selects Cymbidium sinense resources after transplanting of grown-up seedlings, the method provided by the invention completes selection of the easily-industrialized Cymbidium sinense resources in test tubes, so the period of breeding is significantly shortened, and manpower and material resources are saved; meanwhile, the method can really improve the tissue culturing and rapid propagation abilities of traditional Cymbidium sinense, obtains the easily-industrialized breeding Cymbidium sinense resources, realizes industrialized production of Cymbidium sinense seedlings, and has important practical significance on promoting the industrial development of Cymbidium sinense.

Description

technical field [0001] The invention belongs to the field of innovation of plant germplasm resources, and in particular relates to disclosing a method for creating molantium resources that are easy to reproduce in factories. Background technique [0002] Chinese Cymbidium refers to the terrestrial species of orchids in the genus Cymbidium, including Chunlan, Cymbidium, Jianlan, Molan, Hanlan, etc. It has a long history of cultivation and profound cultural connotation in China. The market prospect broad. In 2015, the domestic annual sales of Guolan was 1.224 billion yuan, of which Molan accounted for the highest proportion. With the development of my country's economy, the continuous improvement of people's quality of life and the popularization and promotion of orchid culture, Guolan will become more and more popular among domestic consumers. At the same time, with the gradual prosperity of China, Guolan, as the carrier of Chinese traditional culture, also has a broad inte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H4/001A01H4/008
Inventor 张志胜刘阳郭和蓉李洁铌谢利曾瑞珍
Owner SOUTH CHINA AGRI UNIV
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