A method for improving severe saline-alkali land

A saline-alkali land and soil improvement technology, applied in the field of soil remediation, can solve the problems of little application value and promotion significance, inability to convert saline-alkali land, and reduce the salt content of saline-alkali land, so as to increase soil permeability, improve economic benefits, and improve soil structure effect

Active Publication Date: 2021-09-28
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when the soil salinity is too high, the growth of salt-tolerant plants and halophytes will also be inhibited; and this method can only reduce the salt content of saline-alkali land to a certain extent, and cannot completely convert saline-alkali land into arable land, so , the practical application value and promotion significance are not great

Method used

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  • A method for improving severe saline-alkali land
  • A method for improving severe saline-alkali land
  • A method for improving severe saline-alkali land

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Screening and identification of desired microorganisms

[0050] 1. The medium and reagents involved in this embodiment are as follows:

[0051] (1) Beef extract-peptone liquid medium: 10g peptone, 3g beef extract, 5g sodium chloride, 1000mL distilled water, adjust the pH to 7.0, and sterilize at 121°C for 20min. Add 18 g of agar to the beef extract peptone liquid medium to obtain the beef extract peptone solid medium.

[0052] (2) Ashby nitrogen-free liquid medium: mannitol 10g, CaCO 3 5g, KH 2 PO 3 0.2g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.2g, CaSO 4 2H 2 O 0.1g, pH=7.4, deionized water 1000mL.

[0053] (3) Phosphorus-dissolving liquid medium: glucose 10g, calcium phosphate 5g, ammonium sulfate 0.1g, potassium chloride 0.2g, magnesium chloride heptahydrate 0.25g, distilled water 1000mL, pH 6.8-7.0, autoclaved at 115°C for 30min.

[0054] (4) DF liquid medium: MnSO 4 ·7H 2 O 0.2g, KH 2 PO 4 4.0g, Na 2 HPO 4 6.0g, citric acid 2.0g, glucose 2.0g, sod...

Embodiment 2

[0088] Example 2: Salt-alkali tolerance display of the strains screened

[0089] 1. Preparation of seed solution: Inoculate the strains YZX4 and YX7 screened in Example 1 into beef extract peptone solid medium for activation: culture in a constant temperature incubator at 30°C for 8 hours. Pick an inoculation loop of activated functional strains YZX4 and YX7 into beef extract peptone liquid medium, and cultivate them at 28°C and 180r / min until the logarithmic phase of the strains (determined by growth curve and OD value) to obtain seed liquid.

[0090] 2. Salt tolerance test: Take an inoculation loop of YZX4 and YX7 bacteria (seed liquid) and inoculate them into beef extract peptone liquid containing 0, 20, 50, 70, 100, 120, 150, 200g / L NaCl In the culture medium (pH=7.2), the beef extract peptone liquid culture medium without inoculation was used as a blank control, and cultured on a shaker at 30° C. at 180 r / min for 2 days. The absorbance value (OD) of the culture medium at...

Embodiment 3

[0093] Example 3: Screening of desalting agent in improver

[0094] 1. Prepare 30g / L NaCl solution, measure its pH value and pNa value, the measurement result shows that the pH value is 5.77, and the pNa value is 0.79. Take 50ml of the above solution, mix it with 1.6g of desalting agent evenly, place it in a constant temperature shaker at 30°C and 160r / min, measure the pH value and pNa value of each bottle of solution after 24h. The desalting agents selected in this experiment were D001 resin, AB500 resin, D113 resin and zeolite powder, and each desalting agent was repeated three times.

[0095] 2. The measurement results are shown in Table 3.

[0096] Table 3 shows the measurement results of different desalting agents

[0097] Type of desalination agent pH after treatment Treated pNa D001 1.34±0.02 2.02±0.07 D113 2.28±0.03 1.31±0.03 AB500 1.87±0.01 1.50±0.02 Zeolite powder 6.06±0.03 0.93±0.01

[0098] The results show that the pH...

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Abstract

The invention belongs to the field of soil remediation, and in particular relates to a method for improving severely saline-alkali land. Specifically, a new Bacillus subtilis is provided, and a modifier is prepared by using it. The specific components of the modifier include: 5-10 parts of microbial inoculum, 70-80 parts of organic fertilizer, 10-20 parts of biochar, desalination 5 to 10 doses. And based on the improver, a series of methods for improving saline-alkali land are provided. Using the method provided by the invention has no secondary pollution and is environmentally friendly, and can completely improve the severely saline-alkali land into land that can be normally cultivated.

Description

technical field [0001] The invention belongs to the field of soil restoration, and in particular relates to a method for improving severe saline-alkali land. Background technique [0002] According to statistics from the Food and Agriculture Organization of the United Nations and UNESCO, the area of ​​saline-alkali land in the world is about 957 million hectares, and the area of ​​saline-alkali land in China is about 100 million hectares, and this value is still rising. The accumulation of salinity in soil is the result of the superposition of a series of natural and human factors acting on different temporal and spatial scales. Soil salinization seriously restricts agricultural development, improving and restoring large areas of saline-alkali wasteland resources is of great significance to the development of agricultural production, land management, and ecological environment protection. [0003] At present, the improvement methods of saline-alkali land mainly include phys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/40C12R1/125C09K101/00C09K109/00
Inventor 李东孙晓莹陈意超刘晓风
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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