Production of plant growth-promoting compound bacterial agent based on ecological relationship among strains and its application

A compound bacterial agent and plant technology, which is applied in the field of agricultural microorganisms, can solve the problems of unstable field application effect and difficult to guarantee the biological function of microbial fertilizers.

Active Publication Date: 2022-05-10
NANJING AGRICULTURAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main bottleneck restricting the development of the bio-fertilizer industry is the instability of field application effects. After the functional microorganisms in the fertilizer are applied to the soil, they face the rejection and competition of indigenous microorganisms, and it is difficult to guarantee the stable performance of the biological functions of microbial fertilizers.

Method used

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  • Production of plant growth-promoting compound bacterial agent based on ecological relationship among strains and its application
  • Production of plant growth-promoting compound bacterial agent based on ecological relationship among strains and its application
  • Production of plant growth-promoting compound bacterial agent based on ecological relationship among strains and its application

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Embodiment Construction

[0031] 1. Strain screening and isolation identification

[0032] (1) Primary strain screening and 16S rDNA identification

[0033] Inoculate the strain SQR9-gfp into cucumber potted plants, the inoculation amount is the final concentration of 10 6 CFU / g, take the cucumber root system two weeks later, wash off the soil on the root surface with deionized pure water, and place it on the semi-solid Swarming medium. Under the microscope, SQR9-gfp will emit green fluorescence ( figure 1 ), pick the fusion strains around them, separate them by streaking on the plate, and carry out 16S rDNA sequencing to identify more than 100 strains of bacteria, which are stored in a glycerol tube in a -80°C refrigerator.

[0034] (2) Strain re-screening and phenotypic identification

[0035] Based on the 16S rDNA sequence, a phylogenetic tree was established, and the strains on the same branch of the phylogenetic tree were used to conduct paired strain confrontation experiments using Swarming s...

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Abstract

The invention provides the production of plant growth-promoting compound microbial agent based on the ecological relationship among bacterial strains and its application. A plant growth-promoting composite bacterial agent, which is mixed with the bacterial liquid of Bacillus amyloliquefaciens SQR9, Bacillus amyloliquefaciens RF‑CU‑1, Bacillus lysinica RF‑CU‑2 and Bacillus brevifaciens RF‑CU‑3 made. The bacillus synthetic flora of the invention can cooperate with each other and significantly promote the growth of cucumber plants, and the growth-promoting effect is also significantly improved with the increase of the diversity of the inoculated flora under the condition of aseptic hydroponic culture in the laboratory.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a design method for producing a plant growth-promoting compound microbial agent based on the ecological relationship among bacterial strains and its application. Background technique [0002] Plant rhizosphere microorganisms are considered to be the second genome of plants, which play a key role in the host's nutrient absorption and assisting plants in coping with various biotic and abiotic stresses. The application of plant rhizosphere growth-promoting bacteria to improve crop yield and quality is a modern One of the important trends in the development of green agriculture. At present, the main bottleneck restricting the development of the bio-fertilizer industry is the instability of field application effects. After the functional microorganisms in the fertilizer are applied to the soil, they face the rejection and competition of indigenous microorganisms, and it is dif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01N63/20A01P21/00C12R1/07C12R1/01
CPCC12N1/20A01N63/22A01N63/20
Inventor 沈其荣徐志辉刘妍张瑞福邵佳慧解继驭
Owner NANJING AGRICULTURAL UNIVERSITY
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