Cellulose degradation complex microbial inoculant as well as preparation method and application thereof

A technology of cellulose degradation and compound bacterial agent, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of limiting bacterial species, and achieve the promotion of degradation, plant growth, safety and harmlessness The effect of enhancing the degree of

Active Publication Date: 2022-05-31
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For a long time, most cellulose-degrading bacterial agents are limited to the combination of common microorganisms that are very common in commercialization, which also limits many applications that have high-efficiency degradation capabilities but are not commonly used.

Method used

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  • Cellulose degradation complex microbial inoculant as well as preparation method and application thereof
  • Cellulose degradation complex microbial inoculant as well as preparation method and application thereof
  • Cellulose degradation complex microbial inoculant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: the construction of composite bacterial agent

[0055] Strain antagonism test: five strains of Bacillus mycoides, Serratia marcescens, Corynebacterium glutamicum, and Trichoderma reesei were subcultured and stabilized. and Aspergillus fumigatus (Aspergillus fumigatus) in the CMC-Na solid medium for two-two cross-streaking, when the strains grow into strips, observe whether there is a zone of inhibition at the intersection of different strains, to detect whether the strains can be used in the construction of complex bacterial strains. see results figure 1 (From left to right in the figure are the cross-hatched results of two strains, three strains, four strains and five strains of bacteria). The intersection of each strain grows well, without antagonistic effect, and can be combined and compounded.

[0056] Determination of enzyme activity of complex bacterial strains: random combination of different bacterial strains (combination I-combination IV) with ...

Embodiment 2

[0064] Example 2: Detection of straw degradation effect of compound bacterial agent

[0065] Soak the straw with 1% NaOH solution for 30 minutes to remove the surface wax and increase the contact degree between the cellulose-degrading bacteria and the cellulose component of the straw. Cut the soaked straw into small pieces of about 5 cm, and soak in deionized water for 24 hours to promote the dissolution of available carbon and nitrogen and adjust the moisture content appropriately. The treated straw was oven-dried at 50° C. and stacked in a fermenter, and urea was added to adjust the C / N to 30 to obtain the pretreated straw raw material.

[0066] The experiment consisted of 4 treatments, namely:

[0067]CK treatment: inoculate the liquid enzyme-producing medium equal to the volume of the bacterial agent on the pretreated straw raw material, mix evenly and then ferment continuously.

[0068] EM treatment: Inoculate the EM bacterial agent (purchased from Tai'an Putian Reagent...

Embodiment 3

[0077] Embodiment 3: Composite bacterial agent Ⅳ carrier screening experiment

[0078] In the present invention, 7 kinds of carriers are selected, which are respectively: inorganic materials (activated carbon, zeolite powder, vermiculite, diatomaceous earth) and organic materials (wheat bran, straw powder, peat soil). All kinds of materials were ground and passed through a 30-mesh sieve, sterilized at 121°C for 30 min, and dried for later use. Before inoculating the carrier, the initial number of viable bacteria in each treatment solution was determined. The specific operation is: under aseptic conditions, mix different carriers and compound bacterial agent IV according to the ratio of 10 g carrier to 40 ml bacterial solution, and then culture at 30°C. The culture carrier was sampled at 5h, 10h, 24h, 2d, 4d, 8d, 14d, and 22d respectively, and 0.2g carrier was taken each time, and distilled water was added to make the volume to 5ml, shaken at 120r / min for 15min, and then centr...

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Abstract

The invention discloses a cellulose degradation complex microbial inoculant and a preparation method thereof. The cellulose degradation complex microbial inoculant is prepared from bacillus mycoides, serratia marcescens, corynebacterium glutamicum, trichoderma reesei and aspergillus fumigatus. The total viable count of the complex microbial inoculant is greater than or equal to 3.75 * 10 < 8 > cfu/mL. The complex microbial inoculant provided by the invention can improve the production efficiency of the organic fertilizer taking the high-cellulose-content organic waste as the fermentation raw material, promote the resource utilization of the high-cellulose-content organic waste such as straw and phytophagous animal manure, and provide a technical basis for the production of the high-quality organic fertilizer and the development of an agricultural waste green treatment mode.

Description

technical field [0001] The invention relates to the technical field of microbial bacterial agents, in particular to a cellulose-degrading composite bacterial agent and its preparation method and application. Background technique [0002] Traditional treatment methods such as burning, returning to the field, and feeding livestock and poultry not only fail to make full use of straw resources, but also bring a series of adverse effects such as air pollution, nutrient loss, and even livestock and poultry diseases. Straw has the advantages of rich nutrients and good structure. Using biological means to process and produce organic fertilizer is a feasible green treatment method for this renewable organic resource. However, the main component of straw is cellulose, which is a polymer polysaccharide composed of multiple glucose molecules connected by β-1,4-glycosidic bonds. It has a complex structure and a high degree of crystallization, which is difficult to degrade. It is current...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F17/20C12R1/07C12R1/15C12R1/43C12R1/885C12R1/68
CPCC12N1/20C12N1/14C05F17/20Y02W30/40
Inventor 董元杰张国言贺明荣代兴龙胡国庆
Owner SHANDONG AGRICULTURAL UNIVERSITY
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