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Method for producing protease for promoting growth of plant roots and fruits

A plant root system and growth-promoting technology, which is applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc., can solve problems such as insufficient plant root growth, low fruit yield, and impact on harvest, so as to improve and promote Effect of growth effect, less residue, and reduction of production cost

Inactive Publication Date: 2019-12-10
秦春国
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a protease production method for plant root system and fruit growth promotion in order to solve the problem that the growth of plant root system and fruit growth is often insufficient, which affects its harvest and greatly reduces the yield of fruit

Method used

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  • Method for producing protease for promoting growth of plant roots and fruits
  • Method for producing protease for promoting growth of plant roots and fruits
  • Method for producing protease for promoting growth of plant roots and fruits

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Embodiment one, with reference to figure 1 , a plant root system and fruit growth promoting protease production method, comprising the steps of:

[0046] S1: Collection of stems and leaves of plants of the same or the same genus, select the stems, leaves, tubers and roots of the same plants or plants of the same genus for collection;

[0047] S2: pretreatment, washing and pulverizing the collected stems and leaves, tubers or roots;

[0048] S3: Add phosphate buffer solution, stir to homogenize, add phosphate buffer solution to the pulverized material, and fully stir to form a slurry;

[0049] S4: Obtain supernatant and residue by filtration and centrifugation, filter the homogenate, and centrifuge the filtration to obtain supernatant and residue;

[0050] S5: salting out the supernatant with ammonium sulfate, centrifuging to collect the insolubles in the upper layer, adding ammonium sulfate to the supernatant for salting out, and centrifuging to collect the insolubles...

Embodiment 2

[0053] Embodiment two, refer to figure 2 , a plant root system and fruit growth promoting protease production method, comprising the steps of:

[0054] S1: Collection of stems and leaves of plants of the same or the same genus, select the stems, leaves, tubers and roots of the same plants or plants of the same genus for collection;

[0055]S2: pretreatment, washing and pulverizing the collected stems and leaves, tubers or roots;

[0056] S3: Add phosphate buffer solution, stir to homogenize, add phosphate buffer solution to the pulverized material, and fully stir to form a slurry;

[0057] S4: Obtain supernatant and residue by filtration and centrifugation, filter the homogenate, and centrifuge the filtration to obtain supernatant and residue;

[0058] S8: preparing a medium for fermentation, preparing a medium for fermentation according to a certain proportion according to the residue obtained in S4;

[0059] S9: inoculate the fluorescent pseudomonas package and lipolytic...

Embodiment 3

[0063] Embodiment three, refer to Figure 1~3 , a plant root system and fruit growth promoting protease production method, comprising the steps of:

[0064] S1: Collection of stems and leaves of plants of the same or the same genus, select the stems, leaves, tubers and roots of the same plants or plants of the same genus for collection;

[0065] S2: pretreatment, washing and pulverizing the collected stems and leaves, tubers or roots;

[0066] S3: Add phosphate buffer solution, stir to homogenize, add phosphate buffer solution to the pulverized material, and fully stir to form a slurry;

[0067] S4: Obtain supernatant and residue by filtration and centrifugation, filter the homogenate, and centrifuge the filtration to obtain supernatant and residue;

[0068] S5: salting out the supernatant with ammonium sulfate, centrifuging to collect the insolubles in the upper layer, adding ammonium sulfate to the supernatant for salting out, and centrifuging to collect the insolubles in ...

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Abstract

The invention discloses a method for producing protease for promoting growth of plant roots and fruits. The method comprises the following steps: S1, collecting stems and leaves of the same plant or plants in the same genus, namely selecting stem leaves, tubers and root-tubers of the plant in the same family or same genus, and performing collection; S2, performing pretreatment, namely washing thecollected stem leaves, tubers or root-tubers, and performing grinding treatment; S3, adding a phosphate buffer into the ground material, namely performing stirring and homogenizing, adding a phosphatebuffer into the ground material, performing sufficient stirring and uniform homogenizing; S4, performing filtering and centrifuging to obtain supernate and residues, namely filtering the homogenate,and centrifuging the filtrate to obtain the supernate and residues; and S5, performing ammonium sulfate precipitation on the supernate, namely centrifugally collecting upper insoluble substances, andadding ammonium sulfate into the supernate to perform salt precipitation. In the method, stem leaves, tubers and root-tubers of the plant in the same family or the same genus are collected to produceprotease, so that the effect of a produced finished product on promoting growth of roots and fruits of the plant can be greatly promoted along with good adaptability and small side effect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a production method of protease for plant root system and fruit growth promotion. Background technique [0002] Proteases are a class of enzymes (enzymes) in organisms that can effectively decompose proteins; the decomposition method is to break the peptide bonds that link amino acids into polypeptide chains; small molecular compounds that inhibit protease activity are called protease inhibitors; many viral proteases Inhibitors are very effective antiviral drugs; according to their way of hydrolyzing polypeptides, they can be divided into two types: endopeptidases and exopeptidases. Endopeptidase cuts off the inside of protein molecules to form peptones and peptones with smaller molecular weights. The exopeptidase hydrolyzes the peptide bonds one by one from the free amino or carboxyl end of the protein molecule to release amino acids. The former is aminopeptidase and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50A01N63/00A01P21/00
CPCC12N9/50A01N63/00
Inventor 秦春国
Owner 秦春国
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