Method for preparing fermentation insecticide by taking radix stemonae as main raw material

A technology for preparing insecticides and herbal medicines, which is applied in the fields of bioengineering and fermentation, can solve problems such as economic difficulties in research and development and production of botanical insecticides, influence on the stability of active substances, unfavorable energy-saving environmental risks, etc., and achieve environmental protection , Conducive to extraction, the effect of less harmful microorganisms

Inactive Publication Date: 2017-06-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, botanical pesticides are mostly extracted with organic solvents or decocted, 1) it is not conducive to energy saving and has a greater environmental risk; 2) it affects the stability of active substances and loses active substances; 3) the increase in cost makes development and application difficult, The effect is slow, so it brings great economic difficulties to the development and production of botanical insecticides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for preparing an insecticide by fermenting a basilica as a raw material, comprising the following steps:

[0019] (1) Select fresh and good-quality basil as the main raw material for fermentation.

[0020] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 20% of the volume, add 5% of the total liquid volume % of household garbage and 1% of starchy substances are used as fermentation stock solution for subsequent use.

[0021] (3) preparing LB culture medium, activating the original preservation bacterial strain, 80r / min vibration culture about 48 hours the fermented bacterial liquid that obtains is standby;

[0022] (4) According to the inoculation ratio of 2%, the commercially available Bacillus pumilus, Bacillus thuringiensis and Bacillus amyloliquefaciens H47 were respectively taken in equal proportions and inserted into the fer...

Embodiment 2

[0025] A method for preparing an insecticide by fermenting a basilica as a raw material, comprising the following steps:

[0026] (1) Select fresh and good-quality basil as the main raw material for fermentation.

[0027] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 30% of the volume, add 10% of the total liquid volume % of household garbage and 1% of starchy substances are used as fermentation stock solution for subsequent use.

[0028] (3) Prepare LB medium, activate the original preservation strain, and vibrate at 100r / min for about 48 hours to obtain the fermented bacterial liquid for subsequent use;

[0029] (4) According to the inoculation ratio of 3%, the commercially available Bacillus pumilus, Bacillus thuringiensis and Bacillus amyloliquefaciens H47 were respectively taken in equal proportions and inserted into the fermentation stoc...

Embodiment 3

[0033] A method for preparing an insecticide by fermenting a basilica as a raw material, comprising the following steps:

[0034] (4) Select fresh and good-quality basil as the main raw material for fermentation.

[0035] (5) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 30% of the volume, add 15% of the total liquid volume % of household garbage and 1% of starchy substances are used as fermentation stock solution for subsequent use.

[0036] (6) Prepare LB medium, activate the original preserved bacterial strain, and vibrate at 100r / min for about 48 hours to obtain the fermented bacterial liquid for subsequent use;

[0037] (4) According to the inoculation ratio of 4%, the commercially available Bacillus pumilus, Bacillus thuringiensis and Bacillus amyloliquefaciens H47 were respectively taken in equal proportions and inserted into the fermentati...

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PUM

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Abstract

The invention provides a method for preparing a fermentation insecticide by taking radix stemonae as a main raw material. The technological characteristics are as follows: radix stemonae is taken as the main raw material, a domestic waste, a starch substance and water are taken as auxiliary materials, the commercially available bacillus pumilus, bacillus thuringiensis and bacillus amyloliquefaciens H47 are fermentation strains, the ratio of radix stemonae to the domestic waste to the starch substance to purified water is (2-5):(1-2):0.01:(3-7), the inoculum size of the strain accounts for 2-5% of the system, the fermentation temperature is 25-35 DEG C, the revolving speed is 100-200 rpm, the pH is kept to 5-8, and the fermentation time is 6-9 days. The raw materials are completely fermented, various enzymes, vitamins, amino acids and microelements are fully released, and the effect and the function of the fermentation insecticide are improved. In addition, the physiological activator of the botanical insecticide is not affected.

Description

technical field [0001] A method for preparing insecticides by microbial fermentation, which involves using commercially available Bacillus pumilus, Bacillus thuringiensis and Bacillus amyloliquefaciens H47 freeze-dried powders, and using baccatum as raw materials to ferment and prepare insecticides, which belongs to bioengineering and fermentation technology field. Background technique [0002] Baibu is a perennial herb of the family Centipede, 60-90cm high. Root tubers spindle-shaped, fleshy, in clusters of several or dozens. The lower part of the stem is erect, and the upper part is vine-like. Single leaves in whorls of 2-4 pieces, ovate or ovate-very needle-shaped, apex acute or acuminate, base round or nearly truncate, entire line, leaf veins 5-9, fine and dense transverse veins. The total pedicel is erect, the base is adnate to the midvein of the leaves, and the top is one. Two light green flowers; perianth 4, rolled outward after opening; stamens 4, with appendages...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/46A01P7/04
CPCA01N65/00A01N65/46
Inventor 廖祥儒吴秋兰岑仕宇蔡宇杰管政兵
Owner JIANGNAN UNIV
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