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Phosphorus-solubilizing growth-promoting bacteria Arthrobacter pascens X-1 for promoting growth of root nodules and improving population abundance of probiotic microorganisms and application of phosphorus-solubilizing growth-promoting bacteria Arthrobacter pascens X-1

A probiotic microorganism and Arthrobacter technology, applied in the field of microorganisms, can solve the problems of low effective phosphorus content, low phosphorus diffusion ability, difficult crop absorption and utilization, low phosphorus content, etc., to promote nitrogen fixation by plants, accelerate rock erosion and soil formation, Improves the overall effect of nodules

Active Publication Date: 2021-01-12
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the phosphorus element in the soil is easily oxidized and fixed by iron, aluminum, etc., resulting in low effective phosphorus content. Secondly, the phosphorus element in the soil has a low diffusion ability and is difficult to be absorbed by crops, and it can only be absorbed in the root system of plants, so that crops can absorb it. The phosphorus content is very low

Method used

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  • Phosphorus-solubilizing growth-promoting bacteria Arthrobacter pascens X-1 for promoting growth of root nodules and improving population abundance of probiotic microorganisms and application of phosphorus-solubilizing growth-promoting bacteria Arthrobacter pascens X-1
  • Phosphorus-solubilizing growth-promoting bacteria Arthrobacter pascens X-1 for promoting growth of root nodules and improving population abundance of probiotic microorganisms and application of phosphorus-solubilizing growth-promoting bacteria Arthrobacter pascens X-1
  • Phosphorus-solubilizing growth-promoting bacteria Arthrobacter pascens X-1 for promoting growth of root nodules and improving population abundance of probiotic microorganisms and application of phosphorus-solubilizing growth-promoting bacteria Arthrobacter pascens X-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1 sample source

[0035] Strains were screened from rock samples from a bare rock slope located on Yueyang Avenue, Yueyang City, Hunan Province, China. Rock samples were collected from the upper, middle and lower parts of the slope, and brought back to the laboratory for processing, for compositional analysis of mineral samples and subsequent tests. According to the results of mineral analysis, the main components of the rock samples include the following: K 2 O 3.71%, Na 2 O 1.39%, CaO 0.21%, MgO 1.28%, P 2 o 5 0.11%, Fe 2 o 3 6.81%, Al 2 o 3 15.21%, MnO 0.04%.

[0036] 2 Separation and screening

[0037] 2.1 Medium

[0038] (1) Strain isolation medium: NaCl 0.3g, KCl 0.3g, (NH 4 ) SO 2 0.5g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.3g, Ca 3 (PO 4 )2 5.0g, sucrose 10g, agar 15-20g, deionized water 1000mL, pH 7.0-7.5.

[0039] (2) Beef extract peptone medium: 3 g beef (extract) extract, 10 g peptone, 5 g NaCl, 20 g agar, 1000 m...

Embodiment 2

[0061] Investigate the effect of bacterial growth-promoting effect. Combined with pot experiments, by planting soybeans and observing their growth conditions, a more comprehensive and practical test of the screened strains is carried out to explore the potential effects that can be exerted in the actual environment and production applications.

[0062] Preparation of bacterial agents containing X-1:

[0063] After activating the strain, inoculate it into a liquid medium and ferment for 3 days, and measure the OD with an ultraviolet spectrophotometer 600 , ensure the OD of the bacterial solution by diluting or continuing to ferment 600 The value is in the range of 0.8 to 1.2, and then sealed and stored in a refrigerator at 4°C for use.

[0064] When applying bacteria to potted plants, dilute the stored bacterial solution 100 times, put 60mL diluted bacterial solution into each pot, set up 3 parallels for each treatment, and use sterile medium as a blank control.

[0065] Pup...

Embodiment 3

[0085] Explore the effect of bacterial strains on microbial community composition.

[0086] The collected soybean rhizosphere soil samples were sent to Shanghai Majorbio Bio-pharm Technology Co., Ltd. for sequencing using the IlluminaMiseq platform.

[0087] Detection of microbial diversity in potting soil by high-throughput sequencing, and analysis of microbial community composition in potting soil. Depend on Figure 7 It can be seen that there was no difference in the microbial community composition at the phylum level between the control group and the potting soil treated with the X-1 strain, mainly Proteobacteria and Bacteroidetes. However, there were some differences in the relative abundance of microorganisms among the groups. After treatment with strain X-1, Bradyrhizobium increased from 0.21% to 52.47%, while the dominant phylum Proteobacteri increased from 35.36% to 69.08%. The species composition of the potting soil at the genus level is as follows: Figure 8 As s...

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Abstract

The invention discloses phosphorus-solubilizing growth-promoting bacteria Arthrobacter pascens X-1 for promoting growth of root nodules and improving the population abundance of probiotic microorganisms, and belongs to the technical field of microorganisms. The Arthrobacter pascens X-1 promotes production of soybean plants, promotes proliferation of the root nodules of the plants, and improves theprobiotic microorganisms and the nutritional environment. After the Arthrobacter pascens X-1 is applied, nutrient elements such as potassium, calcium and magnesium required by the plants in rock powder are effectively released, rock erosion soil formation is accelerated, meanwhile, effective nitrogen fixation of soybeans is promoted, nitrogen required by growth of the soybeans is provided, the symbiotic nitrogen fixation capacity of rhizobium and host plants is obviously exerted, the total weight of the root nodules is increased by 150.00%, the content of hydrolyzed nitrogen is increased by 11.58%, and the symbiotic nitrogen fixation capacity is effectively exerted. Meanwhile, the relative abundance of Bradyrhizobium in soil is increased from 0.21% to 52.47%, and the number of the increased relative abundance of the Bradyrhizobium and the promoting effect on plant bodies are superior to the effect of direct application of the Bradyrhizobium.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a halotolerant Arthrobacter X-1 that promotes root nodule growth and increases the abundance of probiotic microbial populations. Background technique [0002] Soybean is a leguminous plant with comprehensive nutrition and rich content. It is most commonly used to make various soy products, extract soybean oil, brew soy sauce and extract protein. It is one of the most important food crops in China and has a history of 5,000 years of cultivation. It is cultivated all over China and is also widely cultivated all over the world. However, China is the world's largest importer of soybeans. It is understood that a large area of ​​my country's land is in a state of obvious phosphorus deficiency, which has seriously restricted the growth of crops, and soil is the only natural channel for crops to obtain phosphorus. Phosphorus plays an important role in plant nutriti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/80C05G3/00C12R1/06
CPCC05G3/80C05G3/00C12R2001/06C12N1/205C05F11/08C12R2001/01C05G3/60C05F11/00Y02P60/21
Inventor 庄家尧刘超徐童心
Owner NANJING FORESTRY UNIV
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