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Application of growth-promoting bacteria fermented by using pinellia ternata as raw material in insect resistance

A technology of pinellia and raw materials, applied in the field of bioengineering and fermentation, can solve problems such as economic difficulties in the research and development and production of botanical insecticides, affect the stability of active substances, unfavorable energy-saving and environmental risks, and achieve good insecticide and disease prevention and Effect of promoting growth, improving the effect of promoting growth, and benefiting the effect of environmental protection

Inactive Publication Date: 2017-07-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, botanical pesticides are mostly extracted with organic solvents or decocted, 1) it is not conducive to energy saving and has a greater environmental risk; 2) it affects the stability of active substances and loses active substances; 3) the increase in cost makes development and application difficult, The effect is slow, so it brings great economic difficulties to the development and production of botanical insecticides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for preparing an insecticide by fermenting pinellia as a raw material, comprising the following steps:

[0019] (1) Select fresh and good-quality pinellia as the main raw material for fermentation.

[0020] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 20% of the volume, add 5% of the total liquid volume % of household garbage and 1% of starchy substances are used as fermentation stock solution for subsequent use.

[0021] (3) Prepare LB medium, activate the original preservation strain, and vibrate at 100r / min for about 48 hours to obtain the fermented bacterial liquid for subsequent use;

[0022] (4) According to the inoculation ratio of 3%, the commercially available Bacillus pumilus, Bacillus thuringiensis and Bacillus amyloliquefaciens H47 were respectively taken and inserted into the fermentation stock solution in equal p...

Embodiment 2

[0025] A method for preparing an insecticide by fermenting pinellia as a raw material, comprising the following steps:

[0026] (1) Select fresh and good-quality pinellia as the main raw material for fermentation.

[0027] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 30% of the volume, add 10% of the total liquid volume % of household garbage and 1% of starchy substances are used as fermentation stock solution for subsequent use.

[0028] (3) Prepare LB medium, activate the original preservation strain, and vibrate at 100r / min for about 48 hours to obtain the fermented bacterial liquid for subsequent use;

[0029] (4) According to different inoculation ratios (commercially available Bacillus pumilus: Bacillus thuringiensis: Bacillus amyloliquefaciens = 2:3:1), the inoculation amount is 2% Commercially available Bacillus pumilus: 3% Bacillus t...

Embodiment 3

[0032] A method for preparing an insecticide by fermenting pinellia as a raw material, comprising the following steps:

[0033] (1) Select fresh and good-quality pinellia as the main raw material for fermentation.

[0034] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 40% of the volume, add 15% of the total liquid volume % of household garbage and 1% of starchy substances are used as fermentation stock solution for subsequent use.

[0035] (3) Prepare LB medium, activate the original preservation strain, and vibrate at 100r / min for about 48 hours to obtain the fermented bacterial liquid for subsequent use;

[0036] (4) According to different inoculation ratios (commercially available Bacillus pumilus: Bacillus thuringiensis: Bacillus amyloliquefaciens = 1:2:3), the inoculation amount was 1% commercially available Bacillus pumilus, 2% Bacillus ...

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Abstract

The invention provides a method for preparing a pesticide by fermenting pinellia ternata. The method is technologically characterized in that the pinellia ternata is used as a raw material and is supplemented by household garbage, starchy materials and water, commercially available bacillus pumilus, bacillus thuringiensis and bacillus amyloliquefaciens H47 are used as fermenting strains, the method is based on the proportion of the pinellia ternata, the household garbage, the starchy materials and the purified water 1-3 to 1-2 to 0.01 to 7-8 and the strain inoculation quantity of 1-3%, the fermentation temperature is 25-35 DEG C, the rotating speed is 100-120 rpm, pH is kept to be 5-8, and fermentation time is 6-9 hours. The fermentation is complete, so that various enzymes, vitamins, amino acids and trace elements are fully released to improve the efficacy and function of the pesticide. In addition, the physiologically active substances of plant pesticides are not affected.

Description

technical field [0001] A method for preparing insecticides by microbial fermentation, which involves using commercially available Bacillus pumilus, Bacillus thuringiensis and Bacillus amyloliquefaciens H47 freeze-dried powder, and using pinellia as raw materials to ferment and prepare insecticides, which belongs to bioengineering and fermentation technology field. Background technique [0002] Pinellia is a perennial herb of the family Araceae. 15-35cm high, tuber nearly spherical, 0.5-3.0cm in diameter, 1-4 basal leaves, leaves from the top of the tuber, petiole 5-25cm long, a white or brown bulbil in the lower part of the petiole, 3-8cm in diameter, occasionally leaves The base also has a white or brown bulbil, 2-4mm in diameter. Flowers unisexual, female flowers below inflorescence rachis, no perianth, 20-70 pistils, short style, monoecious; male flowers located in upper part of inflorescence rachis, white, without peripalum, stamens densely cylindrical, spaced 3 from f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04A01N65/40A01N63/02A01P7/04C12R1/07
CPCA01N65/00A01N65/40A01N63/10C12P1/04C12N1/205C12R2001/07A01N2300/00
Inventor 廖祥儒吴秋兰郑杰蔡宇杰管政兵
Owner JIANGNAN UNIV
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