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Pseudomonas fluorescens pf27 and application thereof in promoting plant growth

A Pseudomonas fluorescens, plant technology, applied in the direction of plant growth regulators, plant growth regulators, applications, etc., can solve problems such as hindering crop transformation and absorption, soil nutrient imbalance, crop nutritional imbalance, etc., to promote sustainable agriculture Development, efficient plant growth, effect of promoting plant growth

Active Publication Date: 2017-10-10
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Excessive application of several chemical fertilizers of a single nature will easily lead to nutritional imbalance of crops. Most of the chemical fertilizers contain various salts, among which chemical substances such as nitrogen, phosphorus, and potassium are easily consolidated by the soil and accumulate in the soil. The salinization of the soil causes the imbalance of soil nutrients, affects and hinders the transformation and absorption of the absorbed nutrients by crops, makes the growth characteristics of fruits and vegetables inferior, the taste and quality of crops deteriorate, and the quality declines. For example, vegetables taste unsavory and fruits taste bad Not sweet, poor taste, and perishable, not suitable for storage

Method used

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  • Pseudomonas fluorescens pf27 and application thereof in promoting plant growth
  • Pseudomonas fluorescens pf27 and application thereof in promoting plant growth
  • Pseudomonas fluorescens pf27 and application thereof in promoting plant growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1. Isolation and identification of pf27 strain

[0054] 1. Isolation of pf27 strain

[0055] pf27 was isolated from the rhizosphere soil of the wild rice area in Pu'er City, Yunnan Province (latitude 22 north 。 33’56", 100 。 35'12”, 737 meters above sea level). The specific separation method is as follows: pick the root system, scrape the rhizosphere soil from the rhizosphere soil collected from the wild rice field, weigh 5g, and put it into the ready under sterile conditions In the sterile Erlenmeyer flask, diluted with sterile water to 10 -6 Times, set 3 repetitions. Take 200μl of the soil samples of gradient dilution and apply them to MEA medium, beef extract peptone medium, LB medium and KB medium plates respectively, and cultivate them in an incubator at 28°C and 37°C. Observe the growth status of colonies every day. The result shows that the dilution is 10 -5 , 10 -6 There are basically no colonies growing on the plate when doubling, and the dilution is 10 -4 ...

Embodiment 2

[0066] Example 2. Preparation of microbial agent pf27

[0067] Pseudomonas fluorescens pf27 obtained in Example 1 was cultured with shaking in KB medium, shaking culture at 28°C and 200rpm for 12-16h, then centrifuged at 4000rpm for 20min, discarding the supernatant, and collecting the bacterial pellet , Then use sterile water to dilute the bacterial pellet to make microbial inoculum pf27 (infectious agent pf27). The total concentration of pf27 viable bacteria in the microbial inoculum pf27 is 1×10 9 -1×10 10 CFU / mL.

Embodiment 3

[0068] Example 3. Growth-promoting effect of microbial agent pf27 on tomato seedlings of Solanaceae crops

[0069] 1. Experimental method

[0070] The tomato seeds of Zhongza No. 9 (produced by China Vegetable Seed Technology (Beijing) Co., Ltd.) are disinfected with 3% NaClO solution for 10 minutes before use, ddH 2 O wash 6 times to get the sterilized seeds. Then spread the sterilized seeds evenly on the sterilized vermiculite peat soil matrix (mix the vermiculite and peat soil in a ratio of 1:1, autoclave at 121°C, 20min) to grow seedlings, and illuminate at 26°C for 12h:12h: Cultivate the seeds to germinate under dark conditions. When the seeds have grown to two true leaves, they are transplanted to the uniform addition of the microbial agent pf27 (the concentration is per kg of vermiculite peat soil matrix mixture is to add 50ml of bacteria liquid with an OD value of 1, The final concentration of the bacterial solution is 1×10 5 CFU / mL) and the control nutrient hole, the toma...

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Abstract

The invention discloses pseudomonas fluorescens pf27, an application thereof in promoting plant growth and a microbial agent pf27 prepared from the pseudomonas fluorescens pf27. Experiments prove that the microbial agent pf27 can remarkably increase the plant height, the root length and the like of plants after being treated and has a certain growth promoting function on plants; comparison between crops treated with the microbial agent pf27 with a control group indicates that the growth promoting effect of the microbial agent pf27 is higher than 21.55% and can be up to 829.14% to the maximum extent, the microbial agent pf27 has a broad-spectrum and efficient plant growth promoting function and especially can promote growth of solanaceous vegetable type crops and improve the quality of the solanaceous vegetable type crops. The problems that chemical fertilizers and other methods are poor in effect or have residues and the like are solved, agricultural sustainable development is promoted, and pseudomonas fluorescens pf27 and the microbial agent pf27 have better application prospects.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a strain of Pseudomonas fluorescens pf27 and its application in plant growth promotion. Background technique [0002] The continuous cropping obstacles caused by intensive production have severely affected the yield and quality of crops, plant physiological characteristics, soil microbial community structure, and rampant plant diseases and insect pests, and seriously affected the efficiency and healthy development of agriculture. Excessive application of chemical fertilizers has caused great harm to the environment and human health, and has become an important factor affecting the environment, human health and social development. In recent years, due to the large-scale production and unrestricted use of chemical pesticides and chemical fertilizers, it has caused pollution to human living environment and agricultural, forestry and animal husbandry products, endangered human and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/39
CPCA01N63/00C12N1/20C12N1/205C12R2001/39
Inventor 叶健姚香梅王端
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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